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Genetic analysis and molecular mapping of QTLs for resistance to rice black-streaked dwarf disease in rice.

Zhou T, Du L, Wang L, Wang Y, Gao C, Lan Y, Sun F, Fan Y, Wang G, Zhou Y - Sci Rep (2015)

Bottom Line: All the resistance loci identified in this study were associated with virus resistance genes.The alleles for enhancing resistance on chromosomes 3 and 11 originated from Tetep, whereas the other allele on chromosome 10 originated from a susceptible parent.The identified new resistance QTLs in this study are useful resources for efficiently breeding resistant rice cultivars to RBSDV.

View Article: PubMed Central - PubMed

Affiliation: 1] Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China [2] Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China.

ABSTRACT
Rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (RBSDV), is transmitted by small brown planthoppers (Laodelphax striatellus Fallén, SBPH) and causes severe yield loss in epidemic years in China and other East Asian countries. Breeding for resistance to RBSDV is a promising strategy to control the disease. We identified Tetep that showed resistance to RBSDV using a field test and artificial inoculation test. An evaluation of the resistance mechanism revealed that Tetep was resistant to RBSDV but not to SBPH. Genetic analysis showed that the resistance of Tetep to RBSDV was controlled by quantitative trait loci (QTLs). Three new QTLs for RBSDV resistance were identified in this study, i.e., qRBSDV-3, qRBSDV-10 and qRBSDV-11. The LOD scores of qRBSDV-3, qRBSDV-10 and qRBSDV-11 were 4.07, 2.24 and 2.21, accounting for 17.5%, 0.3% and 12.4% of the total phenotypic variation, respectively. All the resistance loci identified in this study were associated with virus resistance genes. The alleles for enhancing resistance on chromosomes 3 and 11 originated from Tetep, whereas the other allele on chromosome 10 originated from a susceptible parent. The identified new resistance QTLs in this study are useful resources for efficiently breeding resistant rice cultivars to RBSDV.

No MeSH data available.


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Comparison of the resistant loci around qRBSDV-11.
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f6: Comparison of the resistant loci around qRBSDV-11.

Mentions: qRBSDV-10 mapped to the short arm of chromosome 10 in our study. By comparative mapping using common DNA markers, portions of qRBSDV-10 were found to overlap with the region of qSTV10 that confers RSV resistance20. qRBSDV-11 is located on the long arm of chromosome 11, however, qRBSDV1113 and another two loci reported to confer RBSDV resistance11 mapped to the short arm of chromosome 11. Thus, qRBSDV-11 is a new QTL found in this study. To date, some major QTLs for rice stripe disease resistance have also been identified on chromosome 11, such as qSTV11-f, stv-bi and qStv11202122. According to the comparative mapping analysis, qSTV11-f 20 and qStv1122 were localized to the same region as qRBSDV-11 in this study (Fig. 6). The loci responsible for resistance to different types of pathogens or pests are thought to be clustered in specific chromosomal regions in many organisms20212223242526. Indeed, many reports have demonstrated that the region described above is a hot spot for genes that confer resistance to diseases, including rice blast, rice bacterial leaf blight and rice stripe disease, or pests, including Nephotettix cincticeps, Nephotettix virescens and SBPH. These data indicate that this region might play an important role in the co-evolution between plants and parasites, thus, more attention should be devoted to this region in resistance breeding programs.


Genetic analysis and molecular mapping of QTLs for resistance to rice black-streaked dwarf disease in rice.

Zhou T, Du L, Wang L, Wang Y, Gao C, Lan Y, Sun F, Fan Y, Wang G, Zhou Y - Sci Rep (2015)

Comparison of the resistant loci around qRBSDV-11.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4510485&req=5

f6: Comparison of the resistant loci around qRBSDV-11.
Mentions: qRBSDV-10 mapped to the short arm of chromosome 10 in our study. By comparative mapping using common DNA markers, portions of qRBSDV-10 were found to overlap with the region of qSTV10 that confers RSV resistance20. qRBSDV-11 is located on the long arm of chromosome 11, however, qRBSDV1113 and another two loci reported to confer RBSDV resistance11 mapped to the short arm of chromosome 11. Thus, qRBSDV-11 is a new QTL found in this study. To date, some major QTLs for rice stripe disease resistance have also been identified on chromosome 11, such as qSTV11-f, stv-bi and qStv11202122. According to the comparative mapping analysis, qSTV11-f 20 and qStv1122 were localized to the same region as qRBSDV-11 in this study (Fig. 6). The loci responsible for resistance to different types of pathogens or pests are thought to be clustered in specific chromosomal regions in many organisms20212223242526. Indeed, many reports have demonstrated that the region described above is a hot spot for genes that confer resistance to diseases, including rice blast, rice bacterial leaf blight and rice stripe disease, or pests, including Nephotettix cincticeps, Nephotettix virescens and SBPH. These data indicate that this region might play an important role in the co-evolution between plants and parasites, thus, more attention should be devoted to this region in resistance breeding programs.

Bottom Line: All the resistance loci identified in this study were associated with virus resistance genes.The alleles for enhancing resistance on chromosomes 3 and 11 originated from Tetep, whereas the other allele on chromosome 10 originated from a susceptible parent.The identified new resistance QTLs in this study are useful resources for efficiently breeding resistant rice cultivars to RBSDV.

View Article: PubMed Central - PubMed

Affiliation: 1] Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China [2] Scientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing 210014, China.

ABSTRACT
Rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (RBSDV), is transmitted by small brown planthoppers (Laodelphax striatellus Fallén, SBPH) and causes severe yield loss in epidemic years in China and other East Asian countries. Breeding for resistance to RBSDV is a promising strategy to control the disease. We identified Tetep that showed resistance to RBSDV using a field test and artificial inoculation test. An evaluation of the resistance mechanism revealed that Tetep was resistant to RBSDV but not to SBPH. Genetic analysis showed that the resistance of Tetep to RBSDV was controlled by quantitative trait loci (QTLs). Three new QTLs for RBSDV resistance were identified in this study, i.e., qRBSDV-3, qRBSDV-10 and qRBSDV-11. The LOD scores of qRBSDV-3, qRBSDV-10 and qRBSDV-11 were 4.07, 2.24 and 2.21, accounting for 17.5%, 0.3% and 12.4% of the total phenotypic variation, respectively. All the resistance loci identified in this study were associated with virus resistance genes. The alleles for enhancing resistance on chromosomes 3 and 11 originated from Tetep, whereas the other allele on chromosome 10 originated from a susceptible parent. The identified new resistance QTLs in this study are useful resources for efficiently breeding resistant rice cultivars to RBSDV.

No MeSH data available.


Related in: MedlinePlus