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Psychrophilic fungi from the world's roof.

Wang M, Jiang X, Wu W, Hao Y, Su Y, Cai L, Xiang M, Liu X - Persoonia (2014)

Bottom Line: Psychrophila gen. nov. was therefore established to accommodate these strains which are characterised by globose or subglobose conidia formed from phialides on short or reduced conidiophores.Together with the recognisable morphological distinctions, six new species (i.e. P. antarctica, P. lutea, P. olivacea, T. ellipsoideum, T. globosum and T. psychrophilum) were described.Our preliminary investigation indicates a high diversity of cold-adapted species in nature, and many of them may represent unknown species.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 1st Beichen West Road, Chaoyang District, Beijing 100101, China; ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
During a survey of cold-adapted fungi in alpine glaciers on the Qinghai-Tibet Plateau, 1 428 fungal isolates were obtained of which 150 species were preliminary identified. Phoma sclerotioides and Pseudogymnoascus pannorum were the most dominant species. Psychrotolerant species in Helotiales (Leotiomycetes, Ascomycota) were studied in more detail as they represented the most commonly encountered group during this investigation. Two phylogenetic trees were constructed based on the partial large subunit nrDNA (LSU) to infer the taxonomic placements of these strains. Our strains nested in two well-supported major clades, which represented Tetracladium and a previously unknown lineage. The unknown lineage is distant to any other currently known genera in Helotiales. Psychrophila gen. nov. was therefore established to accommodate these strains which are characterised by globose or subglobose conidia formed from phialides on short or reduced conidiophores. Our analysis also showed that an LSU-based phylogeny is insufficient in differentiating strains at species level. Additional analyses using combined sequences of ITS+TEF1+TUB regions were employed to further investigate the phylogenetic relationships of these strains. Together with the recognisable morphological distinctions, six new species (i.e. P. antarctica, P. lutea, P. olivacea, T. ellipsoideum, T. globosum and T. psychrophilum) were described. Our preliminary investigation indicates a high diversity of cold-adapted species in nature, and many of them may represent unknown species.

No MeSH data available.


Phylogenetic tree derived from maximum parsimony analysis based on LSU rDNA sequences (TL = 610, CI = 0.5607, RI = 0.7609, HI = 0.4393 and RC = 0.4266). Trichoglossum hirsutum OSC61726 was used as outgroup. The LSU alignment consists of 851 characters, with 184 phylogenetically informative positions. Bootstrap values of more than 50 % are shown on the respective branches and significant Bayesian posterior probability (≥ 95 %) are indicated as bold branches. Ex-type cultures are marked with asterisks (*).
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Figure 1: Phylogenetic tree derived from maximum parsimony analysis based on LSU rDNA sequences (TL = 610, CI = 0.5607, RI = 0.7609, HI = 0.4393 and RC = 0.4266). Trichoglossum hirsutum OSC61726 was used as outgroup. The LSU alignment consists of 851 characters, with 184 phylogenetically informative positions. Bootstrap values of more than 50 % are shown on the respective branches and significant Bayesian posterior probability (≥ 95 %) are indicated as bold branches. Ex-type cultures are marked with asterisks (*).

Mentions: The phylogenetic relationships were determined for 16 isolates. According to the phylogenetic trees based on the partial large subunit nrDNA (LSU), 16 isolates clustered into two independent clades, one was strongly supported and well separated from other known genera in the Helotiales (Fig. 1) and should represent a new genus and the other eight isolates clustered within the Tetracladium clade (Fig. 2). In the phylogenetic trees (Fig. 3, 4) generated from combined sequences of ITS+TEF1+TUB, the isolates in Fig. 1 clustered into one clade comprising three subclades that were well supported and separated from each other. Based on phylogenetic relationships and morphological characteristics, a new genus, Psychrophila, is proposed to accommodate these three new species (P. antarctica, P. lutea and P. olivacea).


Psychrophilic fungi from the world's roof.

Wang M, Jiang X, Wu W, Hao Y, Su Y, Cai L, Xiang M, Liu X - Persoonia (2014)

Phylogenetic tree derived from maximum parsimony analysis based on LSU rDNA sequences (TL = 610, CI = 0.5607, RI = 0.7609, HI = 0.4393 and RC = 0.4266). Trichoglossum hirsutum OSC61726 was used as outgroup. The LSU alignment consists of 851 characters, with 184 phylogenetically informative positions. Bootstrap values of more than 50 % are shown on the respective branches and significant Bayesian posterior probability (≥ 95 %) are indicated as bold branches. Ex-type cultures are marked with asterisks (*).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4510274&req=5

Figure 1: Phylogenetic tree derived from maximum parsimony analysis based on LSU rDNA sequences (TL = 610, CI = 0.5607, RI = 0.7609, HI = 0.4393 and RC = 0.4266). Trichoglossum hirsutum OSC61726 was used as outgroup. The LSU alignment consists of 851 characters, with 184 phylogenetically informative positions. Bootstrap values of more than 50 % are shown on the respective branches and significant Bayesian posterior probability (≥ 95 %) are indicated as bold branches. Ex-type cultures are marked with asterisks (*).
Mentions: The phylogenetic relationships were determined for 16 isolates. According to the phylogenetic trees based on the partial large subunit nrDNA (LSU), 16 isolates clustered into two independent clades, one was strongly supported and well separated from other known genera in the Helotiales (Fig. 1) and should represent a new genus and the other eight isolates clustered within the Tetracladium clade (Fig. 2). In the phylogenetic trees (Fig. 3, 4) generated from combined sequences of ITS+TEF1+TUB, the isolates in Fig. 1 clustered into one clade comprising three subclades that were well supported and separated from each other. Based on phylogenetic relationships and morphological characteristics, a new genus, Psychrophila, is proposed to accommodate these three new species (P. antarctica, P. lutea and P. olivacea).

Bottom Line: Psychrophila gen. nov. was therefore established to accommodate these strains which are characterised by globose or subglobose conidia formed from phialides on short or reduced conidiophores.Together with the recognisable morphological distinctions, six new species (i.e. P. antarctica, P. lutea, P. olivacea, T. ellipsoideum, T. globosum and T. psychrophilum) were described.Our preliminary investigation indicates a high diversity of cold-adapted species in nature, and many of them may represent unknown species.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 1st Beichen West Road, Chaoyang District, Beijing 100101, China; ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
During a survey of cold-adapted fungi in alpine glaciers on the Qinghai-Tibet Plateau, 1 428 fungal isolates were obtained of which 150 species were preliminary identified. Phoma sclerotioides and Pseudogymnoascus pannorum were the most dominant species. Psychrotolerant species in Helotiales (Leotiomycetes, Ascomycota) were studied in more detail as they represented the most commonly encountered group during this investigation. Two phylogenetic trees were constructed based on the partial large subunit nrDNA (LSU) to infer the taxonomic placements of these strains. Our strains nested in two well-supported major clades, which represented Tetracladium and a previously unknown lineage. The unknown lineage is distant to any other currently known genera in Helotiales. Psychrophila gen. nov. was therefore established to accommodate these strains which are characterised by globose or subglobose conidia formed from phialides on short or reduced conidiophores. Our analysis also showed that an LSU-based phylogeny is insufficient in differentiating strains at species level. Additional analyses using combined sequences of ITS+TEF1+TUB regions were employed to further investigate the phylogenetic relationships of these strains. Together with the recognisable morphological distinctions, six new species (i.e. P. antarctica, P. lutea, P. olivacea, T. ellipsoideum, T. globosum and T. psychrophilum) were described. Our preliminary investigation indicates a high diversity of cold-adapted species in nature, and many of them may represent unknown species.

No MeSH data available.