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Gardenia jasminoides extracts and gallic acid inhibit lipopolysaccharide-induced inflammation by suppression of JNK2/1 signaling pathways in BV-2 cells.

Lin WH, Kuo HH, Ho LH, Tseng ML, Siao AC, Hung CT, Jeng KC, Hou CW - Iran J Basic Med Sci (2015)

Bottom Line: Our results showed that the GJ extract and GA reduced LPS-induced nitric oxide (NO), interleukin (IL)-1, IL-6, reactive oxygen species (ROS), and prostaglandin (PGE2) production in BV-2 cells.Furthermore, the water extract, but not the ethanol extract, of the GJ dose-dependently inhibited LPS-induced JNK2/1 and slightly p38 mitogen-activated protein kinases (MAPK), and cyclooxygenase-2 (COX-2) expression in BV-2 cells.Taken together, these results indicate that the protective mechanism of the GJ extract involves an antioxidant effect and inhibition of JNK2/1 MAP kinase and COX-2 expressions in LPS-induced inflammation of BV-2 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Technology Management, Chung Hua University, Hsinchu, Taiwan.

ABSTRACT

Objectives: Gardenia jasminoides Ellis (GJ, Cape Jasmine Fruit, Zhi Zi) has been traditionally used for the treatment of infectious hepatitis, aphthous ulcer, and trauma; however, the direct evidence is lacking.

Materials and methods: We investigated the effect of the GJ extract (GJ) and gallic acid (GA) on lipopolysaccharide (LPS) induced inflammation of BV-2 microglial cells and acute liver injury in Sprague-Dawley (SD) rats.

Results: Our results showed that the GJ extract and GA reduced LPS-induced nitric oxide (NO), interleukin (IL)-1, IL-6, reactive oxygen species (ROS), and prostaglandin (PGE2) production in BV-2 cells. The GJ extract and GA significantly decreased serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in LPS-treated rats. Furthermore, the water extract, but not the ethanol extract, of the GJ dose-dependently inhibited LPS-induced JNK2/1 and slightly p38 mitogen-activated protein kinases (MAPK), and cyclooxygenase-2 (COX-2) expression in BV-2 cells.

Conclusion: Taken together, these results indicate that the protective mechanism of the GJ extract involves an antioxidant effect and inhibition of JNK2/1 MAP kinase and COX-2 expressions in LPS-induced inflammation of BV-2 cells.

No MeSH data available.


Related in: MedlinePlus

The effect of the Gardenia jasminoides (GJ) extract and gallic acid (GA) on cell signaling was investigated in BV-2 cells. The GJ extract dose-dependently inhibited LPS-induced JNK2/1, partially p38 MAPK, and inhibited COX-2 in BV-2 cells. GA (1 μM) only inhibited LPS-induced JNK2/1 MAPK partially
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Figure 6: The effect of the Gardenia jasminoides (GJ) extract and gallic acid (GA) on cell signaling was investigated in BV-2 cells. The GJ extract dose-dependently inhibited LPS-induced JNK2/1, partially p38 MAPK, and inhibited COX-2 in BV-2 cells. GA (1 μM) only inhibited LPS-induced JNK2/1 MAPK partially

Mentions: The effect of GJ extract and GA on cell signaling was investigated both in vitro and in vivo. The GJ extract dose-dependently inhibited LPS-induced JNK, and p38 MAPK, and partially inhibited COX-2 in BV-2 cells. Similarly, the GJ extract inhibited LPS-induced p38 MAPK, and partially inhibited COX-2 in the livers of SD rats (Figure 6).


Gardenia jasminoides extracts and gallic acid inhibit lipopolysaccharide-induced inflammation by suppression of JNK2/1 signaling pathways in BV-2 cells.

Lin WH, Kuo HH, Ho LH, Tseng ML, Siao AC, Hung CT, Jeng KC, Hou CW - Iran J Basic Med Sci (2015)

The effect of the Gardenia jasminoides (GJ) extract and gallic acid (GA) on cell signaling was investigated in BV-2 cells. The GJ extract dose-dependently inhibited LPS-induced JNK2/1, partially p38 MAPK, and inhibited COX-2 in BV-2 cells. GA (1 μM) only inhibited LPS-induced JNK2/1 MAPK partially
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4509950&req=5

Figure 6: The effect of the Gardenia jasminoides (GJ) extract and gallic acid (GA) on cell signaling was investigated in BV-2 cells. The GJ extract dose-dependently inhibited LPS-induced JNK2/1, partially p38 MAPK, and inhibited COX-2 in BV-2 cells. GA (1 μM) only inhibited LPS-induced JNK2/1 MAPK partially
Mentions: The effect of GJ extract and GA on cell signaling was investigated both in vitro and in vivo. The GJ extract dose-dependently inhibited LPS-induced JNK, and p38 MAPK, and partially inhibited COX-2 in BV-2 cells. Similarly, the GJ extract inhibited LPS-induced p38 MAPK, and partially inhibited COX-2 in the livers of SD rats (Figure 6).

Bottom Line: Our results showed that the GJ extract and GA reduced LPS-induced nitric oxide (NO), interleukin (IL)-1, IL-6, reactive oxygen species (ROS), and prostaglandin (PGE2) production in BV-2 cells.Furthermore, the water extract, but not the ethanol extract, of the GJ dose-dependently inhibited LPS-induced JNK2/1 and slightly p38 mitogen-activated protein kinases (MAPK), and cyclooxygenase-2 (COX-2) expression in BV-2 cells.Taken together, these results indicate that the protective mechanism of the GJ extract involves an antioxidant effect and inhibition of JNK2/1 MAP kinase and COX-2 expressions in LPS-induced inflammation of BV-2 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Technology Management, Chung Hua University, Hsinchu, Taiwan.

ABSTRACT

Objectives: Gardenia jasminoides Ellis (GJ, Cape Jasmine Fruit, Zhi Zi) has been traditionally used for the treatment of infectious hepatitis, aphthous ulcer, and trauma; however, the direct evidence is lacking.

Materials and methods: We investigated the effect of the GJ extract (GJ) and gallic acid (GA) on lipopolysaccharide (LPS) induced inflammation of BV-2 microglial cells and acute liver injury in Sprague-Dawley (SD) rats.

Results: Our results showed that the GJ extract and GA reduced LPS-induced nitric oxide (NO), interleukin (IL)-1, IL-6, reactive oxygen species (ROS), and prostaglandin (PGE2) production in BV-2 cells. The GJ extract and GA significantly decreased serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in LPS-treated rats. Furthermore, the water extract, but not the ethanol extract, of the GJ dose-dependently inhibited LPS-induced JNK2/1 and slightly p38 mitogen-activated protein kinases (MAPK), and cyclooxygenase-2 (COX-2) expression in BV-2 cells.

Conclusion: Taken together, these results indicate that the protective mechanism of the GJ extract involves an antioxidant effect and inhibition of JNK2/1 MAP kinase and COX-2 expressions in LPS-induced inflammation of BV-2 cells.

No MeSH data available.


Related in: MedlinePlus