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The effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on intracellular Ca2+ handling in rat ventricular myocytes.

Miller L, Greensmith DJ, Sankaranarayanan R, O'Neill SC, Eisner DA - Cell Calcium (2015)

Bottom Line: SR Ca content was also reduced by 62±4%.However, at concentrations that decrease SERCA activity, TBQ also decreases the L-type Ca current and (at higher concentrations) activates an outward current which appears to be an ATP dependent potassium current.We conclude that TBQ cannot be used as a specific inhibitor of SERCA in rat ventricular myocytes.

View Article: PubMed Central - PubMed

Affiliation: Unit of Cardiac Physiology, Institute of Cardiovascular Sciences, 3.06 Core Technology Facility, 46 Grafton Street, Manchester M13 9NT, United Kingdom.

No MeSH data available.


Related in: MedlinePlus

The effect of TBQ on SR Ca content. (A) Specimen INCX records (top panel) and corresponding integrals (lower panel) in control (left) and following 10 μM TBQ (right) evoked by rapid application of 10 mM caffeine (white bar). (B) Average SR Ca (n = 5 cells from 3 animals, *p < 0.05 vs. control).
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fig0010: The effect of TBQ on SR Ca content. (A) Specimen INCX records (top panel) and corresponding integrals (lower panel) in control (left) and following 10 μM TBQ (right) evoked by rapid application of 10 mM caffeine (white bar). (B) Average SR Ca (n = 5 cells from 3 animals, *p < 0.05 vs. control).

Mentions: 10 μM TBQ was used to study the effect on SR Ca content. This concentration was used as it decreased SERCA activity without markedly reducing the systolic Ca transient. Fig. 2A (representative of 5 cells) shows specimen NCX currents (top) produced by rapid application of 10 mM caffeine in control and following TBQ and the integrals of those currents (below). TBQ consistently decreased this integral. On average (Fig. 2B) TBQ decreased SR Ca content by 62 ± 4%.


The effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on intracellular Ca2+ handling in rat ventricular myocytes.

Miller L, Greensmith DJ, Sankaranarayanan R, O'Neill SC, Eisner DA - Cell Calcium (2015)

The effect of TBQ on SR Ca content. (A) Specimen INCX records (top panel) and corresponding integrals (lower panel) in control (left) and following 10 μM TBQ (right) evoked by rapid application of 10 mM caffeine (white bar). (B) Average SR Ca (n = 5 cells from 3 animals, *p < 0.05 vs. control).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4509554&req=5

fig0010: The effect of TBQ on SR Ca content. (A) Specimen INCX records (top panel) and corresponding integrals (lower panel) in control (left) and following 10 μM TBQ (right) evoked by rapid application of 10 mM caffeine (white bar). (B) Average SR Ca (n = 5 cells from 3 animals, *p < 0.05 vs. control).
Mentions: 10 μM TBQ was used to study the effect on SR Ca content. This concentration was used as it decreased SERCA activity without markedly reducing the systolic Ca transient. Fig. 2A (representative of 5 cells) shows specimen NCX currents (top) produced by rapid application of 10 mM caffeine in control and following TBQ and the integrals of those currents (below). TBQ consistently decreased this integral. On average (Fig. 2B) TBQ decreased SR Ca content by 62 ± 4%.

Bottom Line: SR Ca content was also reduced by 62±4%.However, at concentrations that decrease SERCA activity, TBQ also decreases the L-type Ca current and (at higher concentrations) activates an outward current which appears to be an ATP dependent potassium current.We conclude that TBQ cannot be used as a specific inhibitor of SERCA in rat ventricular myocytes.

View Article: PubMed Central - PubMed

Affiliation: Unit of Cardiac Physiology, Institute of Cardiovascular Sciences, 3.06 Core Technology Facility, 46 Grafton Street, Manchester M13 9NT, United Kingdom.

No MeSH data available.


Related in: MedlinePlus