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Hyaluronic acid-functionalized single-walled carbon nanotubes as tumor-targeting MRI contrast agent.

Hou L, Zhang H, Wang Y, Wang L, Yang X, Zhang Z - Int J Nanomedicine (2015)

Bottom Line: The safety evaluation of this MRI CAs was investigated in vitro and in vivo.It revealed that HA-SWCNTs could stand as a biocompatible nanocarrier and gadolinium (Gd)/HA-SWCNTs demonstrated almost no toxicity compared with free GdCl3.Moreover, GdCl3 bearing HA-SWCNTs could significantly increase the circulation time for MRI.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.

ABSTRACT
A tumor-targeting carrier, hyaluronic acid (HA)-functionalized single-walled carbon nanotubes (SWCNTs), was explored to deliver magnetic resonance imaging (MRI) contrast agents (CAs) targeting to the tumor cells specifically. In this system, HA surface modification for SWCNTs was simply accomplished by amidation process and could make this nanomaterial highly hydrophilic. Cellular uptake was performed to evaluate the intracellular transport capabilities of HA-SWCNTs for tumor cells and the uptake rank was HA-SWCNTs> SWCNTs owing to the presence of HA, which was also evidenced by flow cytometry. The safety evaluation of this MRI CAs was investigated in vitro and in vivo. It revealed that HA-SWCNTs could stand as a biocompatible nanocarrier and gadolinium (Gd)/HA-SWCNTs demonstrated almost no toxicity compared with free GdCl3. Moreover, GdCl3 bearing HA-SWCNTs could significantly increase the circulation time for MRI. Finally, to investigate the MRI contrast enhancing capabilities of Gd/HA-SWCNTs, T1-weighted MR images of tumor-bearing mice were acquired. The results suggested Gd/HA-SWCNTs had the highest tumor-targeting efficiency and T1-relaxivity enhancement, indicating HA-SWCNTs could be developed as a tumor-targeting carrier to deliver the CAs, GdCl3, for the identifiable diagnosis of tumor.

No MeSH data available.


Related in: MedlinePlus

Histologic assessments of liver (A) and kidney (B) with H&E staining in BALB/c mice.Abbreviations: Gd-DTPA, gadolinium (III)-diethylenediaminepentaacetic acid; Gd/HA-SWCNTs, gadolinium/hyaluronic acid-functionalized single-walled carbon nanotubes; Gd/SWCNTs, gadolinium/single-walled carbon nanotubes; H&E, hematoxylin–eosin.
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f8-ijn-10-4507: Histologic assessments of liver (A) and kidney (B) with H&E staining in BALB/c mice.Abbreviations: Gd-DTPA, gadolinium (III)-diethylenediaminepentaacetic acid; Gd/HA-SWCNTs, gadolinium/hyaluronic acid-functionalized single-walled carbon nanotubes; Gd/SWCNTs, gadolinium/single-walled carbon nanotubes; H&E, hematoxylin–eosin.

Mentions: Moreover, it demonstrated negligible systematic toxicity through the histopathological analysis, especially in liver and kidney (Figure 8). As shown in Figure 8, at the 1st day after administration, the hepatocytes were intact and cell nucleus staining was obvious in Gd/SWCNTs and Gd/HA-SWCNTs groups, showing almost no toxicity. While Gd-DTPA group exhibited slight toxicity with balloon-like cells emerging and GdCl3 group presented acute toxicity with increased balloon-like cells and necrotic liver cell debris. The renal toxicity results showed that no significant pathological changes were observed in Gd/SWCNTs, Gd/HA-SWCNTs, and Gd-DTPA groups, while GdCl3 group presented slight toxicity, suggesting Gd/HA-SWCNTs was a relatively safe MRI CA in vivo. The physiological samples of the 5th day and 10th day showed that there were no obvious pathological changes in liver and kidney of Gd/SWCNTs, Gd/HA-SWCNTs, and Gd-DTPA groups, while GdCl3 group also exhibited certain degree of liver toxicity. The aforementioned situation may be related with the metabolism of CAs and hepatic tissue regeneration.


Hyaluronic acid-functionalized single-walled carbon nanotubes as tumor-targeting MRI contrast agent.

Hou L, Zhang H, Wang Y, Wang L, Yang X, Zhang Z - Int J Nanomedicine (2015)

Histologic assessments of liver (A) and kidney (B) with H&E staining in BALB/c mice.Abbreviations: Gd-DTPA, gadolinium (III)-diethylenediaminepentaacetic acid; Gd/HA-SWCNTs, gadolinium/hyaluronic acid-functionalized single-walled carbon nanotubes; Gd/SWCNTs, gadolinium/single-walled carbon nanotubes; H&E, hematoxylin–eosin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4509541&req=5

f8-ijn-10-4507: Histologic assessments of liver (A) and kidney (B) with H&E staining in BALB/c mice.Abbreviations: Gd-DTPA, gadolinium (III)-diethylenediaminepentaacetic acid; Gd/HA-SWCNTs, gadolinium/hyaluronic acid-functionalized single-walled carbon nanotubes; Gd/SWCNTs, gadolinium/single-walled carbon nanotubes; H&E, hematoxylin–eosin.
Mentions: Moreover, it demonstrated negligible systematic toxicity through the histopathological analysis, especially in liver and kidney (Figure 8). As shown in Figure 8, at the 1st day after administration, the hepatocytes were intact and cell nucleus staining was obvious in Gd/SWCNTs and Gd/HA-SWCNTs groups, showing almost no toxicity. While Gd-DTPA group exhibited slight toxicity with balloon-like cells emerging and GdCl3 group presented acute toxicity with increased balloon-like cells and necrotic liver cell debris. The renal toxicity results showed that no significant pathological changes were observed in Gd/SWCNTs, Gd/HA-SWCNTs, and Gd-DTPA groups, while GdCl3 group presented slight toxicity, suggesting Gd/HA-SWCNTs was a relatively safe MRI CA in vivo. The physiological samples of the 5th day and 10th day showed that there were no obvious pathological changes in liver and kidney of Gd/SWCNTs, Gd/HA-SWCNTs, and Gd-DTPA groups, while GdCl3 group also exhibited certain degree of liver toxicity. The aforementioned situation may be related with the metabolism of CAs and hepatic tissue regeneration.

Bottom Line: The safety evaluation of this MRI CAs was investigated in vitro and in vivo.It revealed that HA-SWCNTs could stand as a biocompatible nanocarrier and gadolinium (Gd)/HA-SWCNTs demonstrated almost no toxicity compared with free GdCl3.Moreover, GdCl3 bearing HA-SWCNTs could significantly increase the circulation time for MRI.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.

ABSTRACT
A tumor-targeting carrier, hyaluronic acid (HA)-functionalized single-walled carbon nanotubes (SWCNTs), was explored to deliver magnetic resonance imaging (MRI) contrast agents (CAs) targeting to the tumor cells specifically. In this system, HA surface modification for SWCNTs was simply accomplished by amidation process and could make this nanomaterial highly hydrophilic. Cellular uptake was performed to evaluate the intracellular transport capabilities of HA-SWCNTs for tumor cells and the uptake rank was HA-SWCNTs> SWCNTs owing to the presence of HA, which was also evidenced by flow cytometry. The safety evaluation of this MRI CAs was investigated in vitro and in vivo. It revealed that HA-SWCNTs could stand as a biocompatible nanocarrier and gadolinium (Gd)/HA-SWCNTs demonstrated almost no toxicity compared with free GdCl3. Moreover, GdCl3 bearing HA-SWCNTs could significantly increase the circulation time for MRI. Finally, to investigate the MRI contrast enhancing capabilities of Gd/HA-SWCNTs, T1-weighted MR images of tumor-bearing mice were acquired. The results suggested Gd/HA-SWCNTs had the highest tumor-targeting efficiency and T1-relaxivity enhancement, indicating HA-SWCNTs could be developed as a tumor-targeting carrier to deliver the CAs, GdCl3, for the identifiable diagnosis of tumor.

No MeSH data available.


Related in: MedlinePlus