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Hyaluronic acid-functionalized single-walled carbon nanotubes as tumor-targeting MRI contrast agent.

Hou L, Zhang H, Wang Y, Wang L, Yang X, Zhang Z - Int J Nanomedicine (2015)

Bottom Line: The safety evaluation of this MRI CAs was investigated in vitro and in vivo.It revealed that HA-SWCNTs could stand as a biocompatible nanocarrier and gadolinium (Gd)/HA-SWCNTs demonstrated almost no toxicity compared with free GdCl3.Moreover, GdCl3 bearing HA-SWCNTs could significantly increase the circulation time for MRI.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.

ABSTRACT
A tumor-targeting carrier, hyaluronic acid (HA)-functionalized single-walled carbon nanotubes (SWCNTs), was explored to deliver magnetic resonance imaging (MRI) contrast agents (CAs) targeting to the tumor cells specifically. In this system, HA surface modification for SWCNTs was simply accomplished by amidation process and could make this nanomaterial highly hydrophilic. Cellular uptake was performed to evaluate the intracellular transport capabilities of HA-SWCNTs for tumor cells and the uptake rank was HA-SWCNTs> SWCNTs owing to the presence of HA, which was also evidenced by flow cytometry. The safety evaluation of this MRI CAs was investigated in vitro and in vivo. It revealed that HA-SWCNTs could stand as a biocompatible nanocarrier and gadolinium (Gd)/HA-SWCNTs demonstrated almost no toxicity compared with free GdCl3. Moreover, GdCl3 bearing HA-SWCNTs could significantly increase the circulation time for MRI. Finally, to investigate the MRI contrast enhancing capabilities of Gd/HA-SWCNTs, T1-weighted MR images of tumor-bearing mice were acquired. The results suggested Gd/HA-SWCNTs had the highest tumor-targeting efficiency and T1-relaxivity enhancement, indicating HA-SWCNTs could be developed as a tumor-targeting carrier to deliver the CAs, GdCl3, for the identifiable diagnosis of tumor.

No MeSH data available.


Related in: MedlinePlus

Cell viability of MCF-7 cells with different concentrations of Gd3+.Abbreviations: Gd/HA-SWCNTs, gadolinium/hyaluronic acid-functionalized single-walled carbon nanotubes; Gd-DTPA, gadolinium (III)-diethylenediaminepentaacetic acid; Gd/SWCNTs, gadolinium/single-walled carbon nanotubes.
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f7-ijn-10-4507: Cell viability of MCF-7 cells with different concentrations of Gd3+.Abbreviations: Gd/HA-SWCNTs, gadolinium/hyaluronic acid-functionalized single-walled carbon nanotubes; Gd-DTPA, gadolinium (III)-diethylenediaminepentaacetic acid; Gd/SWCNTs, gadolinium/single-walled carbon nanotubes.

Mentions: The safety evaluation of this MRI CAs was investigated in vitro and in vivo. Cytotoxicity of the Gd/HA-SWCNTs was an important consideration for materials intended for in vivo application. Toward this goal, assessment of cell viability of MCF-7 cancer cells was examined. As shown in Figure 7, in vitro cytotoxicity study revealed that the cytotoxicity of Gd/HA-SWCNTs was lower than Gd/SWCNTs at all concentrations, indicating that the modification of HA could improve biocompatibility of SWCNTs. Meanwhile, Gd-DTPA, a MRI CA commonly used in clinical practice, was chosen as contrast reagent to evaluate the effect of Gd/HA-SWCNTs. Results demonstrated that at the same concentration of Gd, the cytotoxicity of Gd/HA-SWCNTs was much lower than Gd-DTPA, suggesting HA-SWCNTs could stand as a biocompatible nanocarrier and Gd/HA-SWCNTs would be a relatively safe MRI CA in future.


Hyaluronic acid-functionalized single-walled carbon nanotubes as tumor-targeting MRI contrast agent.

Hou L, Zhang H, Wang Y, Wang L, Yang X, Zhang Z - Int J Nanomedicine (2015)

Cell viability of MCF-7 cells with different concentrations of Gd3+.Abbreviations: Gd/HA-SWCNTs, gadolinium/hyaluronic acid-functionalized single-walled carbon nanotubes; Gd-DTPA, gadolinium (III)-diethylenediaminepentaacetic acid; Gd/SWCNTs, gadolinium/single-walled carbon nanotubes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4509541&req=5

f7-ijn-10-4507: Cell viability of MCF-7 cells with different concentrations of Gd3+.Abbreviations: Gd/HA-SWCNTs, gadolinium/hyaluronic acid-functionalized single-walled carbon nanotubes; Gd-DTPA, gadolinium (III)-diethylenediaminepentaacetic acid; Gd/SWCNTs, gadolinium/single-walled carbon nanotubes.
Mentions: The safety evaluation of this MRI CAs was investigated in vitro and in vivo. Cytotoxicity of the Gd/HA-SWCNTs was an important consideration for materials intended for in vivo application. Toward this goal, assessment of cell viability of MCF-7 cancer cells was examined. As shown in Figure 7, in vitro cytotoxicity study revealed that the cytotoxicity of Gd/HA-SWCNTs was lower than Gd/SWCNTs at all concentrations, indicating that the modification of HA could improve biocompatibility of SWCNTs. Meanwhile, Gd-DTPA, a MRI CA commonly used in clinical practice, was chosen as contrast reagent to evaluate the effect of Gd/HA-SWCNTs. Results demonstrated that at the same concentration of Gd, the cytotoxicity of Gd/HA-SWCNTs was much lower than Gd-DTPA, suggesting HA-SWCNTs could stand as a biocompatible nanocarrier and Gd/HA-SWCNTs would be a relatively safe MRI CA in future.

Bottom Line: The safety evaluation of this MRI CAs was investigated in vitro and in vivo.It revealed that HA-SWCNTs could stand as a biocompatible nanocarrier and gadolinium (Gd)/HA-SWCNTs demonstrated almost no toxicity compared with free GdCl3.Moreover, GdCl3 bearing HA-SWCNTs could significantly increase the circulation time for MRI.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.

ABSTRACT
A tumor-targeting carrier, hyaluronic acid (HA)-functionalized single-walled carbon nanotubes (SWCNTs), was explored to deliver magnetic resonance imaging (MRI) contrast agents (CAs) targeting to the tumor cells specifically. In this system, HA surface modification for SWCNTs was simply accomplished by amidation process and could make this nanomaterial highly hydrophilic. Cellular uptake was performed to evaluate the intracellular transport capabilities of HA-SWCNTs for tumor cells and the uptake rank was HA-SWCNTs> SWCNTs owing to the presence of HA, which was also evidenced by flow cytometry. The safety evaluation of this MRI CAs was investigated in vitro and in vivo. It revealed that HA-SWCNTs could stand as a biocompatible nanocarrier and gadolinium (Gd)/HA-SWCNTs demonstrated almost no toxicity compared with free GdCl3. Moreover, GdCl3 bearing HA-SWCNTs could significantly increase the circulation time for MRI. Finally, to investigate the MRI contrast enhancing capabilities of Gd/HA-SWCNTs, T1-weighted MR images of tumor-bearing mice were acquired. The results suggested Gd/HA-SWCNTs had the highest tumor-targeting efficiency and T1-relaxivity enhancement, indicating HA-SWCNTs could be developed as a tumor-targeting carrier to deliver the CAs, GdCl3, for the identifiable diagnosis of tumor.

No MeSH data available.


Related in: MedlinePlus