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Effects of a micro/nano rough strontium-loaded surface on osseointegration.

Li Y, Qi Y, Gao Q, Niu Q, Shen M, Fu Q, Hu K, Kong L - Int J Nanomedicine (2015)

Bottom Line: The results showed that MNT-Sr surfaces were prepared successfully and with high interface bonding strength.Compared with the MT surface, the NT-Sr surface significantly improved the differentiation of osteoblasts in vitro.In the in vivo animal experiment, the MT surface significantly enhanced the bone-implant contact and maximal pullout force than the NT-Sr surface.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi'an, People's Republic of China.

ABSTRACT
We developed a hierarchical hybrid micro/nanorough strontium-loaded Ti (MNT-Sr) surface fabricated through hydrofluoric acid etching followed by magnetron sputtering and evaluated the effects of this surface on osseointegration. Samples with a smooth Ti (ST) surface, micro Ti (MT) surface treated with hydrofluoric acid etching, and strontium-loaded nano Ti (NT-Sr) surface treated with SrTiO3 target deposited via magnetron sputtering technique were investigated in parallel for comparison. The results showed that MNT-Sr surfaces were prepared successfully and with high interface bonding strength. Moreover, slow Sr release could be detected when the MNT-Sr and NT-Sr samples were immersed in phosphate-buffered saline. In in vitro experiments, the MNT-Sr surface significantly improved the proliferation and differentiation of osteoblasts compared with the other three groups. Twelve weeks after the four different surface implants were inserted into the distal femurs of 40 rats, the bone-implant contact in the ST, MT, NT-Sr, and MNT-Sr groups were 39.70%±6.00%, 57.60%±7.79%, 46.10%±5.51%, and 70.38%±8.61%, respectively. In terms of the mineral apposition ratio, the MNT-Sr group increased by 129%, 58%, and 25% compared with the values of the ST, MT, and NT-Sr groups, respectively. Moreover, the maximal pullout force in the MNT-Sr group was 1.12-, 0.31-, and 0.69-fold higher than the values of the ST, MT, and NT-Sr groups, respectively. These results suggested that the MNT-Sr surface has a synergistic effect of hierarchical micro/nano-topography and strontium for enhanced osseointegration, and it may be a promising option for clinical use. Compared with the MT surface, the NT-Sr surface significantly improved the differentiation of osteoblasts in vitro. In the in vivo animal experiment, the MT surface significantly enhanced the bone-implant contact and maximal pullout force than the NT-Sr surface.

No MeSH data available.


Related in: MedlinePlus

Representative CLSM images of cells stained with DAPI to show the nuclei (blue) and FITC to show the actin filaments (green).Notes: (A) ST; (B) MT; (C) NT-Sr; and (D) MNT-Sr.Abbreviations: CLSM, confocal laser scanning microscope; FITC, fluorescein isothiocyanate; MNT-Sr, micro/nanorough strontium-loaded Ti; MT, micro titanium surface; NT-Sr, nano strontium-containing titanium surface; ST, smooth titanium surface.
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f9-ijn-10-4549: Representative CLSM images of cells stained with DAPI to show the nuclei (blue) and FITC to show the actin filaments (green).Notes: (A) ST; (B) MT; (C) NT-Sr; and (D) MNT-Sr.Abbreviations: CLSM, confocal laser scanning microscope; FITC, fluorescein isothiocyanate; MNT-Sr, micro/nanorough strontium-loaded Ti; MT, micro titanium surface; NT-Sr, nano strontium-containing titanium surface; ST, smooth titanium surface.

Mentions: The CLSM images in Figure 9 show osteoblasts stained with FITC–phalloidin to label the actin cytoskeleton. More microfilaments and stress fibers (thicker bundles of filaments) were observed in the MNT-Sr sample. Strong labeling was frequently observed along the cellular edge and within the filopodia. In contrast, although the cells on the MT surface were extended with many filopodia, few cellular junctions were observed, and most of the cells did not exhibit an aligned cell array or a well-developed cytoskeleton. The actin bundles exhibited frequent haphazard orientation, and few long stress fibers were visible. The cells on the MT and NT-Sr surfaces exhibited more stress fibers and actin microfilaments than those on the ST surface did. At the same time, obvious cytoskeletal networks were observed on the MNT-Sr surface.


Effects of a micro/nano rough strontium-loaded surface on osseointegration.

Li Y, Qi Y, Gao Q, Niu Q, Shen M, Fu Q, Hu K, Kong L - Int J Nanomedicine (2015)

Representative CLSM images of cells stained with DAPI to show the nuclei (blue) and FITC to show the actin filaments (green).Notes: (A) ST; (B) MT; (C) NT-Sr; and (D) MNT-Sr.Abbreviations: CLSM, confocal laser scanning microscope; FITC, fluorescein isothiocyanate; MNT-Sr, micro/nanorough strontium-loaded Ti; MT, micro titanium surface; NT-Sr, nano strontium-containing titanium surface; ST, smooth titanium surface.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4509532&req=5

f9-ijn-10-4549: Representative CLSM images of cells stained with DAPI to show the nuclei (blue) and FITC to show the actin filaments (green).Notes: (A) ST; (B) MT; (C) NT-Sr; and (D) MNT-Sr.Abbreviations: CLSM, confocal laser scanning microscope; FITC, fluorescein isothiocyanate; MNT-Sr, micro/nanorough strontium-loaded Ti; MT, micro titanium surface; NT-Sr, nano strontium-containing titanium surface; ST, smooth titanium surface.
Mentions: The CLSM images in Figure 9 show osteoblasts stained with FITC–phalloidin to label the actin cytoskeleton. More microfilaments and stress fibers (thicker bundles of filaments) were observed in the MNT-Sr sample. Strong labeling was frequently observed along the cellular edge and within the filopodia. In contrast, although the cells on the MT surface were extended with many filopodia, few cellular junctions were observed, and most of the cells did not exhibit an aligned cell array or a well-developed cytoskeleton. The actin bundles exhibited frequent haphazard orientation, and few long stress fibers were visible. The cells on the MT and NT-Sr surfaces exhibited more stress fibers and actin microfilaments than those on the ST surface did. At the same time, obvious cytoskeletal networks were observed on the MNT-Sr surface.

Bottom Line: The results showed that MNT-Sr surfaces were prepared successfully and with high interface bonding strength.Compared with the MT surface, the NT-Sr surface significantly improved the differentiation of osteoblasts in vitro.In the in vivo animal experiment, the MT surface significantly enhanced the bone-implant contact and maximal pullout force than the NT-Sr surface.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi'an, People's Republic of China.

ABSTRACT
We developed a hierarchical hybrid micro/nanorough strontium-loaded Ti (MNT-Sr) surface fabricated through hydrofluoric acid etching followed by magnetron sputtering and evaluated the effects of this surface on osseointegration. Samples with a smooth Ti (ST) surface, micro Ti (MT) surface treated with hydrofluoric acid etching, and strontium-loaded nano Ti (NT-Sr) surface treated with SrTiO3 target deposited via magnetron sputtering technique were investigated in parallel for comparison. The results showed that MNT-Sr surfaces were prepared successfully and with high interface bonding strength. Moreover, slow Sr release could be detected when the MNT-Sr and NT-Sr samples were immersed in phosphate-buffered saline. In in vitro experiments, the MNT-Sr surface significantly improved the proliferation and differentiation of osteoblasts compared with the other three groups. Twelve weeks after the four different surface implants were inserted into the distal femurs of 40 rats, the bone-implant contact in the ST, MT, NT-Sr, and MNT-Sr groups were 39.70%±6.00%, 57.60%±7.79%, 46.10%±5.51%, and 70.38%±8.61%, respectively. In terms of the mineral apposition ratio, the MNT-Sr group increased by 129%, 58%, and 25% compared with the values of the ST, MT, and NT-Sr groups, respectively. Moreover, the maximal pullout force in the MNT-Sr group was 1.12-, 0.31-, and 0.69-fold higher than the values of the ST, MT, and NT-Sr groups, respectively. These results suggested that the MNT-Sr surface has a synergistic effect of hierarchical micro/nano-topography and strontium for enhanced osseointegration, and it may be a promising option for clinical use. Compared with the MT surface, the NT-Sr surface significantly improved the differentiation of osteoblasts in vitro. In the in vivo animal experiment, the MT surface significantly enhanced the bone-implant contact and maximal pullout force than the NT-Sr surface.

No MeSH data available.


Related in: MedlinePlus