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The in vitro Effect of Irrigants with Low Surface Tension on Enterococcus faecalis.

Giardino L, Estrela C, Generali L, Mohammadi Z, Asgary S - Iran Endod J (2015)

Bottom Line: The level of significance was set at 0.05.The results indicated the presence of E. faecalis in all post-irrigation samples irrespective of the RCI.However, the optical densities in both post-irrigation periods showed bacterial reduction and significant differences between groups.

View Article: PubMed Central - PubMed

Affiliation: Researcher, Crotone, Italy;

ABSTRACT

Introduction: Due to the complex anatomy of the root canal system and high surface tension of common root canal irrigants (RCI), conducting an investigation on RCIs containing surfactants is a priority. The aim of this in vitro study was to verify the antibacterial potential of RCI with low surface tension in root canals infected with Enterococcus faecalis (E. faecalis).

Methods and materials: Thirty-five extracted human maxillary anterior teeth were prepared and inoculated with E. faecalis for 60 days. After root canal preparation, the teeth were randomly divided to one positive and one negative control groups and 5 experimental groups: Hypoclean/Tetraclean NA, Hypoclean, Tetraclean, NaOCl/Tetraclean and NaOCl. Bacterial growth was observed by turbidity of culture medium and then measured using a UV spectrophotometer. Data were analyzed in three time intervals (pre-instrumentation and, 20 min and 72 h after canal preparation) using the ANOVA and post hoc Tukey's tests. The level of significance was set at 0.05.

Results: The results indicated the presence of E. faecalis in all post-irrigation samples irrespective of the RCI. However, the optical densities in both post-irrigation periods showed bacterial reduction and significant differences between groups.

Conclusion: RCI with low surface tension showed antibacterial potential in E. faecalis infected roots.

No MeSH data available.


Related in: MedlinePlus

A scanning electron microscopy image of 60-day positive control samples with Enterococcus Faecalis (5000× magnification
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Figure 1: A scanning electron microscopy image of 60-day positive control samples with Enterococcus Faecalis (5000× magnification

Mentions: A split platform was used during inoculation with the bacteria [24]. Briefly, the coronal portion of the root canal was connected to the cut end of a 1.5 mL polypropylene Eppendorf tube using a cyanoacrylate adhesive. The entire surface of tooth-tube interface were coated with nail polish. The specimens were sterilized in 5% NaOCl for 30 min and then were placed in the BHI medium. To ensure disinfection, the test apparatus was incubated at 37°C for 24 h. Using sterilized syringes, the specimens were inoculated with E. faecalis every three days during a 60-day period (Figure 1). The teeth were kept in a humid environment at 37°C.


The in vitro Effect of Irrigants with Low Surface Tension on Enterococcus faecalis.

Giardino L, Estrela C, Generali L, Mohammadi Z, Asgary S - Iran Endod J (2015)

A scanning electron microscopy image of 60-day positive control samples with Enterococcus Faecalis (5000× magnification
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4509125&req=5

Figure 1: A scanning electron microscopy image of 60-day positive control samples with Enterococcus Faecalis (5000× magnification
Mentions: A split platform was used during inoculation with the bacteria [24]. Briefly, the coronal portion of the root canal was connected to the cut end of a 1.5 mL polypropylene Eppendorf tube using a cyanoacrylate adhesive. The entire surface of tooth-tube interface were coated with nail polish. The specimens were sterilized in 5% NaOCl for 30 min and then were placed in the BHI medium. To ensure disinfection, the test apparatus was incubated at 37°C for 24 h. Using sterilized syringes, the specimens were inoculated with E. faecalis every three days during a 60-day period (Figure 1). The teeth were kept in a humid environment at 37°C.

Bottom Line: The level of significance was set at 0.05.The results indicated the presence of E. faecalis in all post-irrigation samples irrespective of the RCI.However, the optical densities in both post-irrigation periods showed bacterial reduction and significant differences between groups.

View Article: PubMed Central - PubMed

Affiliation: Researcher, Crotone, Italy;

ABSTRACT

Introduction: Due to the complex anatomy of the root canal system and high surface tension of common root canal irrigants (RCI), conducting an investigation on RCIs containing surfactants is a priority. The aim of this in vitro study was to verify the antibacterial potential of RCI with low surface tension in root canals infected with Enterococcus faecalis (E. faecalis).

Methods and materials: Thirty-five extracted human maxillary anterior teeth were prepared and inoculated with E. faecalis for 60 days. After root canal preparation, the teeth were randomly divided to one positive and one negative control groups and 5 experimental groups: Hypoclean/Tetraclean NA, Hypoclean, Tetraclean, NaOCl/Tetraclean and NaOCl. Bacterial growth was observed by turbidity of culture medium and then measured using a UV spectrophotometer. Data were analyzed in three time intervals (pre-instrumentation and, 20 min and 72 h after canal preparation) using the ANOVA and post hoc Tukey's tests. The level of significance was set at 0.05.

Results: The results indicated the presence of E. faecalis in all post-irrigation samples irrespective of the RCI. However, the optical densities in both post-irrigation periods showed bacterial reduction and significant differences between groups.

Conclusion: RCI with low surface tension showed antibacterial potential in E. faecalis infected roots.

No MeSH data available.


Related in: MedlinePlus