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Different Effects of Orbital Shear Stress on Vascular Endothelial Cells: Comparison with the Results of In Vivo Study with Rats.

Kim H, Yang KH, Cho H, Gwak G, Park SC, Kim JI, Yun SS, Moon IS - Vasc Specialist Int (2015)

Bottom Line: An attempt was made to characterize the orbital shear stress by comparing the effects of orbital shear stress on vascular endothelial cells (ECs) with the results of animal experiments.Orbital shear stress did not show the characteristics of a pure turbulent shear force.By comparing the observation with the morphological changes of vascular ECs and site-specific protein expression on the results of animal experiments, uniform directional lamina shear stress forces were expressed at the periphery.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: An attempt was made to characterize the orbital shear stress by comparing the effects of orbital shear stress on vascular endothelial cells (ECs) with the results of animal experiments.

Materials and methods: In the laboratory study, cultured ECs of well were distinguished by center and periphery then exposed to orbital shear stress using an orbital shaker. In the animal study, arteriovenous (AV) fistulas were made at the right femoral arteries of Sprague-Dawley rats to increase the effect of the laminar flow. The condition of the stenosis was given on the left femoral arteries. The protein expression of inducible nitric oxide synthase (iNOS) and Akt phosphorylation were observed and compared.

Results: Under orbital shear stress, ECs showed an increase in iNOS protein expression and phosphorylation of Akt but most of the protein expressions derived from the periphery. When compared to the animal study, the increased expression of iNOS protein and phosphorylation of Akt were observed in the sample of AV fistula conditions and the iNOS protein expression was decreased in the stenosis conditions.

Conclusion: Orbital shear stress did not show the characteristics of a pure turbulent shear force. By comparing the observation with the morphological changes of vascular ECs and site-specific protein expression on the results of animal experiments, uniform directional lamina shear stress forces were expressed at the periphery.

No MeSH data available.


Related in: MedlinePlus

Akt phosphorylation with orbital shear stress and in rat model. Bar graph represents the mean relative ratio of phosphorylated to total Akt. A representative Western blot is shown. The relative difference in Akt phosphorylation between periphery and center cells exposed to orbital shear stress is significant (P=0.0002, post hoc). In rat model, arteriovenous (AV) fistula side specimen demonstrate significantly increased Akt phosphorylation compared with stenosis side specimen (P<0.0001, post hoc).
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f4-vsi-31-33: Akt phosphorylation with orbital shear stress and in rat model. Bar graph represents the mean relative ratio of phosphorylated to total Akt. A representative Western blot is shown. The relative difference in Akt phosphorylation between periphery and center cells exposed to orbital shear stress is significant (P=0.0002, post hoc). In rat model, arteriovenous (AV) fistula side specimen demonstrate significantly increased Akt phosphorylation compared with stenosis side specimen (P<0.0001, post hoc).

Mentions: Endothelial cells under an orbital shear stress showed an increased phosphorylation of Akt when compared to a static condition (100±4.9 vs. 248.6±23.7, P<0.05). The Akt phosphorylation of the peripheral endothelial cells increased significantly (377.2±33.8, P<0.05) when compared to the control group. In contrast, there was no significant change in the endothelial cells of the central portion (127.3±22.7) (Fig. 4).


Different Effects of Orbital Shear Stress on Vascular Endothelial Cells: Comparison with the Results of In Vivo Study with Rats.

Kim H, Yang KH, Cho H, Gwak G, Park SC, Kim JI, Yun SS, Moon IS - Vasc Specialist Int (2015)

Akt phosphorylation with orbital shear stress and in rat model. Bar graph represents the mean relative ratio of phosphorylated to total Akt. A representative Western blot is shown. The relative difference in Akt phosphorylation between periphery and center cells exposed to orbital shear stress is significant (P=0.0002, post hoc). In rat model, arteriovenous (AV) fistula side specimen demonstrate significantly increased Akt phosphorylation compared with stenosis side specimen (P<0.0001, post hoc).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4508651&req=5

f4-vsi-31-33: Akt phosphorylation with orbital shear stress and in rat model. Bar graph represents the mean relative ratio of phosphorylated to total Akt. A representative Western blot is shown. The relative difference in Akt phosphorylation between periphery and center cells exposed to orbital shear stress is significant (P=0.0002, post hoc). In rat model, arteriovenous (AV) fistula side specimen demonstrate significantly increased Akt phosphorylation compared with stenosis side specimen (P<0.0001, post hoc).
Mentions: Endothelial cells under an orbital shear stress showed an increased phosphorylation of Akt when compared to a static condition (100±4.9 vs. 248.6±23.7, P<0.05). The Akt phosphorylation of the peripheral endothelial cells increased significantly (377.2±33.8, P<0.05) when compared to the control group. In contrast, there was no significant change in the endothelial cells of the central portion (127.3±22.7) (Fig. 4).

Bottom Line: An attempt was made to characterize the orbital shear stress by comparing the effects of orbital shear stress on vascular endothelial cells (ECs) with the results of animal experiments.Orbital shear stress did not show the characteristics of a pure turbulent shear force.By comparing the observation with the morphological changes of vascular ECs and site-specific protein expression on the results of animal experiments, uniform directional lamina shear stress forces were expressed at the periphery.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: An attempt was made to characterize the orbital shear stress by comparing the effects of orbital shear stress on vascular endothelial cells (ECs) with the results of animal experiments.

Materials and methods: In the laboratory study, cultured ECs of well were distinguished by center and periphery then exposed to orbital shear stress using an orbital shaker. In the animal study, arteriovenous (AV) fistulas were made at the right femoral arteries of Sprague-Dawley rats to increase the effect of the laminar flow. The condition of the stenosis was given on the left femoral arteries. The protein expression of inducible nitric oxide synthase (iNOS) and Akt phosphorylation were observed and compared.

Results: Under orbital shear stress, ECs showed an increase in iNOS protein expression and phosphorylation of Akt but most of the protein expressions derived from the periphery. When compared to the animal study, the increased expression of iNOS protein and phosphorylation of Akt were observed in the sample of AV fistula conditions and the iNOS protein expression was decreased in the stenosis conditions.

Conclusion: Orbital shear stress did not show the characteristics of a pure turbulent shear force. By comparing the observation with the morphological changes of vascular ECs and site-specific protein expression on the results of animal experiments, uniform directional lamina shear stress forces were expressed at the periphery.

No MeSH data available.


Related in: MedlinePlus