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Different Effects of Orbital Shear Stress on Vascular Endothelial Cells: Comparison with the Results of In Vivo Study with Rats.

Kim H, Yang KH, Cho H, Gwak G, Park SC, Kim JI, Yun SS, Moon IS - Vasc Specialist Int (2015)

Bottom Line: An attempt was made to characterize the orbital shear stress by comparing the effects of orbital shear stress on vascular endothelial cells (ECs) with the results of animal experiments.Orbital shear stress did not show the characteristics of a pure turbulent shear force.By comparing the observation with the morphological changes of vascular ECs and site-specific protein expression on the results of animal experiments, uniform directional lamina shear stress forces were expressed at the periphery.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: An attempt was made to characterize the orbital shear stress by comparing the effects of orbital shear stress on vascular endothelial cells (ECs) with the results of animal experiments.

Materials and methods: In the laboratory study, cultured ECs of well were distinguished by center and periphery then exposed to orbital shear stress using an orbital shaker. In the animal study, arteriovenous (AV) fistulas were made at the right femoral arteries of Sprague-Dawley rats to increase the effect of the laminar flow. The condition of the stenosis was given on the left femoral arteries. The protein expression of inducible nitric oxide synthase (iNOS) and Akt phosphorylation were observed and compared.

Results: Under orbital shear stress, ECs showed an increase in iNOS protein expression and phosphorylation of Akt but most of the protein expressions derived from the periphery. When compared to the animal study, the increased expression of iNOS protein and phosphorylation of Akt were observed in the sample of AV fistula conditions and the iNOS protein expression was decreased in the stenosis conditions.

Conclusion: Orbital shear stress did not show the characteristics of a pure turbulent shear force. By comparing the observation with the morphological changes of vascular ECs and site-specific protein expression on the results of animal experiments, uniform directional lamina shear stress forces were expressed at the periphery.

No MeSH data available.


Related in: MedlinePlus

Morphology of cultured endothelial cells (ECs) in vitro after 5 days. (A) Control ECs (static condition). (B) ECs exposed to orbital shear stress (center of the well). (C) ECs exposed to orbital shear stress (periphery of the well). The edge of the culture well is to the right of the panel. (A–C) Stained with crystal violet (×400).
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f2-vsi-31-33: Morphology of cultured endothelial cells (ECs) in vitro after 5 days. (A) Control ECs (static condition). (B) ECs exposed to orbital shear stress (center of the well). (C) ECs exposed to orbital shear stress (periphery of the well). The edge of the culture well is to the right of the panel. (A–C) Stained with crystal violet (×400).

Mentions: The endothelial cells of the peripheral portion were elongated and aligned with the direction of shear stress, as shown in a normal laminar shear force. The endothelial cells of the central portion, similar with the endothelial cells in static condition, were round and randomly aligned (Fig. 2).


Different Effects of Orbital Shear Stress on Vascular Endothelial Cells: Comparison with the Results of In Vivo Study with Rats.

Kim H, Yang KH, Cho H, Gwak G, Park SC, Kim JI, Yun SS, Moon IS - Vasc Specialist Int (2015)

Morphology of cultured endothelial cells (ECs) in vitro after 5 days. (A) Control ECs (static condition). (B) ECs exposed to orbital shear stress (center of the well). (C) ECs exposed to orbital shear stress (periphery of the well). The edge of the culture well is to the right of the panel. (A–C) Stained with crystal violet (×400).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508651&req=5

f2-vsi-31-33: Morphology of cultured endothelial cells (ECs) in vitro after 5 days. (A) Control ECs (static condition). (B) ECs exposed to orbital shear stress (center of the well). (C) ECs exposed to orbital shear stress (periphery of the well). The edge of the culture well is to the right of the panel. (A–C) Stained with crystal violet (×400).
Mentions: The endothelial cells of the peripheral portion were elongated and aligned with the direction of shear stress, as shown in a normal laminar shear force. The endothelial cells of the central portion, similar with the endothelial cells in static condition, were round and randomly aligned (Fig. 2).

Bottom Line: An attempt was made to characterize the orbital shear stress by comparing the effects of orbital shear stress on vascular endothelial cells (ECs) with the results of animal experiments.Orbital shear stress did not show the characteristics of a pure turbulent shear force.By comparing the observation with the morphological changes of vascular ECs and site-specific protein expression on the results of animal experiments, uniform directional lamina shear stress forces were expressed at the periphery.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: An attempt was made to characterize the orbital shear stress by comparing the effects of orbital shear stress on vascular endothelial cells (ECs) with the results of animal experiments.

Materials and methods: In the laboratory study, cultured ECs of well were distinguished by center and periphery then exposed to orbital shear stress using an orbital shaker. In the animal study, arteriovenous (AV) fistulas were made at the right femoral arteries of Sprague-Dawley rats to increase the effect of the laminar flow. The condition of the stenosis was given on the left femoral arteries. The protein expression of inducible nitric oxide synthase (iNOS) and Akt phosphorylation were observed and compared.

Results: Under orbital shear stress, ECs showed an increase in iNOS protein expression and phosphorylation of Akt but most of the protein expressions derived from the periphery. When compared to the animal study, the increased expression of iNOS protein and phosphorylation of Akt were observed in the sample of AV fistula conditions and the iNOS protein expression was decreased in the stenosis conditions.

Conclusion: Orbital shear stress did not show the characteristics of a pure turbulent shear force. By comparing the observation with the morphological changes of vascular ECs and site-specific protein expression on the results of animal experiments, uniform directional lamina shear stress forces were expressed at the periphery.

No MeSH data available.


Related in: MedlinePlus