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Salvianolic acid A as a multifunctional agent ameliorates doxorubicin-induced nephropathy in rats.

Fan HY, Yang MY, Qi D, Zhang ZK, Zhu L, Shang-Guan XX, Liu K, Xu H, Che X - Sci Rep (2015)

Bottom Line: Evidence suggest that multidrug therapy has more effect than monotherapy in amelioration of renal injury.Results revealed that treatment with SAA alleviated histological damages, relieved proteinuria, hypoalbuminemia and hyperlipidemia, reduced oxidative stress, as well as improving hemorheology.The anti-inflammation, antioxidant, amelioration of podocyte injury, improvement of hemorheology and hypolipidemic properties may constituent an important part of its therapeutic effects.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Yantai University, 264005 Yantai, Shandong Province, China.

ABSTRACT
Nephrotic syndrome (NS) is still a therapeutic challenge. To date there is no ideal treatment. Evidence suggest that multidrug therapy has more effect than monotherapy in amelioration of renal injury. Salvianolic acid A (SAA) is the major active component of Salviae Miltiorrhizae Bunge. Previous studies have demonstrated that SAA is a multi-target agent and has various pharmacological activities. The pleiotropic properties of SAA predict its potential in the treatment of NS. The study investigated the effect of SAA on doxorubicin-induced nephropathy. The kidney function related-biochemical changes, hemorheological parameters and oxidative stress status were determined, and histological examination using light and transmission electron microcopies and western blot analysis were also performed. Results revealed that treatment with SAA alleviated histological damages, relieved proteinuria, hypoalbuminemia and hyperlipidemia, reduced oxidative stress, as well as improving hemorheology. Furthermore, SAA restored podocin expression, down-regulated the expression of NF-κB p65 and p-IκBα while up-regulating IκBα protein expression. Overall, as a multifunctional agent, SAA has a favorable renoprotection in doxorubicin-induced nephropathy. The anti-inflammation, antioxidant, amelioration of podocyte injury, improvement of hemorheology and hypolipidemic properties may constituent an important part of its therapeutic effects. All these indicate that SAA is likely to be a promising agent for NS.

No MeSH data available.


Related in: MedlinePlus

The effect of SAA on renal pathology in doxorubicin-induced nephropathy rats.a: Renal histopathologic features; b: Pathological scores of renal tissues of each group. Rats were treated as described in the legend of Fig. 1. At the end of the experiment, rats were sacrificed and renal tissue was fixed in 10% formalin. Histopathological analysis was performed in HE-stained sections of renal. Data are expressed as mean ± SD (n = 3). ##P < 0.01 vs. Control; **P < 0.01 vs. DOX alone. Original magnification, ×100. Abbreviations: SAA, Salvianolic acid A; DOX, Doxorubicin.
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f3: The effect of SAA on renal pathology in doxorubicin-induced nephropathy rats.a: Renal histopathologic features; b: Pathological scores of renal tissues of each group. Rats were treated as described in the legend of Fig. 1. At the end of the experiment, rats were sacrificed and renal tissue was fixed in 10% formalin. Histopathological analysis was performed in HE-stained sections of renal. Data are expressed as mean ± SD (n = 3). ##P < 0.01 vs. Control; **P < 0.01 vs. DOX alone. Original magnification, ×100. Abbreviations: SAA, Salvianolic acid A; DOX, Doxorubicin.

Mentions: Figure 3 shows representative light microscopy images of renal cortex of each group. There were no histopathological changes in the renal tissue of control rats. Histologic evaluation of the kidney of DOX-treated rats showed marked pathological lesions characterized by glomerular epithelial hyperplasia, tubular dilatation and abundant protein exudation in renal tubular lumen and numerous inflammatory cells infiltrations in renal interstitial. Histopathologic injury scores were significantly elevated (P = 0.000). Fortunately, prednisone acetate and SAA with different doses could attenuate the severity of pathological damages in a certain degree, in the form of reduction of inflammatory cells infiltrations and protein cast formation. The pathological scores were correspondingly decreased, and the effect was significant in the groups of SAA 5 and 10 mg/kg and prednisone acetate (P = 0.003, P = 0.000 and P = 0.000, respectively).


Salvianolic acid A as a multifunctional agent ameliorates doxorubicin-induced nephropathy in rats.

Fan HY, Yang MY, Qi D, Zhang ZK, Zhu L, Shang-Guan XX, Liu K, Xu H, Che X - Sci Rep (2015)

The effect of SAA on renal pathology in doxorubicin-induced nephropathy rats.a: Renal histopathologic features; b: Pathological scores of renal tissues of each group. Rats were treated as described in the legend of Fig. 1. At the end of the experiment, rats were sacrificed and renal tissue was fixed in 10% formalin. Histopathological analysis was performed in HE-stained sections of renal. Data are expressed as mean ± SD (n = 3). ##P < 0.01 vs. Control; **P < 0.01 vs. DOX alone. Original magnification, ×100. Abbreviations: SAA, Salvianolic acid A; DOX, Doxorubicin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508635&req=5

f3: The effect of SAA on renal pathology in doxorubicin-induced nephropathy rats.a: Renal histopathologic features; b: Pathological scores of renal tissues of each group. Rats were treated as described in the legend of Fig. 1. At the end of the experiment, rats were sacrificed and renal tissue was fixed in 10% formalin. Histopathological analysis was performed in HE-stained sections of renal. Data are expressed as mean ± SD (n = 3). ##P < 0.01 vs. Control; **P < 0.01 vs. DOX alone. Original magnification, ×100. Abbreviations: SAA, Salvianolic acid A; DOX, Doxorubicin.
Mentions: Figure 3 shows representative light microscopy images of renal cortex of each group. There were no histopathological changes in the renal tissue of control rats. Histologic evaluation of the kidney of DOX-treated rats showed marked pathological lesions characterized by glomerular epithelial hyperplasia, tubular dilatation and abundant protein exudation in renal tubular lumen and numerous inflammatory cells infiltrations in renal interstitial. Histopathologic injury scores were significantly elevated (P = 0.000). Fortunately, prednisone acetate and SAA with different doses could attenuate the severity of pathological damages in a certain degree, in the form of reduction of inflammatory cells infiltrations and protein cast formation. The pathological scores were correspondingly decreased, and the effect was significant in the groups of SAA 5 and 10 mg/kg and prednisone acetate (P = 0.003, P = 0.000 and P = 0.000, respectively).

Bottom Line: Evidence suggest that multidrug therapy has more effect than monotherapy in amelioration of renal injury.Results revealed that treatment with SAA alleviated histological damages, relieved proteinuria, hypoalbuminemia and hyperlipidemia, reduced oxidative stress, as well as improving hemorheology.The anti-inflammation, antioxidant, amelioration of podocyte injury, improvement of hemorheology and hypolipidemic properties may constituent an important part of its therapeutic effects.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Yantai University, 264005 Yantai, Shandong Province, China.

ABSTRACT
Nephrotic syndrome (NS) is still a therapeutic challenge. To date there is no ideal treatment. Evidence suggest that multidrug therapy has more effect than monotherapy in amelioration of renal injury. Salvianolic acid A (SAA) is the major active component of Salviae Miltiorrhizae Bunge. Previous studies have demonstrated that SAA is a multi-target agent and has various pharmacological activities. The pleiotropic properties of SAA predict its potential in the treatment of NS. The study investigated the effect of SAA on doxorubicin-induced nephropathy. The kidney function related-biochemical changes, hemorheological parameters and oxidative stress status were determined, and histological examination using light and transmission electron microcopies and western blot analysis were also performed. Results revealed that treatment with SAA alleviated histological damages, relieved proteinuria, hypoalbuminemia and hyperlipidemia, reduced oxidative stress, as well as improving hemorheology. Furthermore, SAA restored podocin expression, down-regulated the expression of NF-κB p65 and p-IκBα while up-regulating IκBα protein expression. Overall, as a multifunctional agent, SAA has a favorable renoprotection in doxorubicin-induced nephropathy. The anti-inflammation, antioxidant, amelioration of podocyte injury, improvement of hemorheology and hypolipidemic properties may constituent an important part of its therapeutic effects. All these indicate that SAA is likely to be a promising agent for NS.

No MeSH data available.


Related in: MedlinePlus