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Salvianolic acid A as a multifunctional agent ameliorates doxorubicin-induced nephropathy in rats.

Fan HY, Yang MY, Qi D, Zhang ZK, Zhu L, Shang-Guan XX, Liu K, Xu H, Che X - Sci Rep (2015)

Bottom Line: Evidence suggest that multidrug therapy has more effect than monotherapy in amelioration of renal injury.Results revealed that treatment with SAA alleviated histological damages, relieved proteinuria, hypoalbuminemia and hyperlipidemia, reduced oxidative stress, as well as improving hemorheology.The anti-inflammation, antioxidant, amelioration of podocyte injury, improvement of hemorheology and hypolipidemic properties may constituent an important part of its therapeutic effects.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Yantai University, 264005 Yantai, Shandong Province, China.

ABSTRACT
Nephrotic syndrome (NS) is still a therapeutic challenge. To date there is no ideal treatment. Evidence suggest that multidrug therapy has more effect than monotherapy in amelioration of renal injury. Salvianolic acid A (SAA) is the major active component of Salviae Miltiorrhizae Bunge. Previous studies have demonstrated that SAA is a multi-target agent and has various pharmacological activities. The pleiotropic properties of SAA predict its potential in the treatment of NS. The study investigated the effect of SAA on doxorubicin-induced nephropathy. The kidney function related-biochemical changes, hemorheological parameters and oxidative stress status were determined, and histological examination using light and transmission electron microcopies and western blot analysis were also performed. Results revealed that treatment with SAA alleviated histological damages, relieved proteinuria, hypoalbuminemia and hyperlipidemia, reduced oxidative stress, as well as improving hemorheology. Furthermore, SAA restored podocin expression, down-regulated the expression of NF-κB p65 and p-IκBα while up-regulating IκBα protein expression. Overall, as a multifunctional agent, SAA has a favorable renoprotection in doxorubicin-induced nephropathy. The anti-inflammation, antioxidant, amelioration of podocyte injury, improvement of hemorheology and hypolipidemic properties may constituent an important part of its therapeutic effects. All these indicate that SAA is likely to be a promising agent for NS.

No MeSH data available.


Related in: MedlinePlus

The effects of SAA on superoxide dismutase (SOD) activity and level of malondialdehyde (MDA) in doxorubicin-induced nephropathy rats.a: SOD activity, b: MDA level. Rats were treated as described in the legend of Fig. 1. At the end of the experiment, rats were sacrificed and renal cortical tissue were collected for determination of SOD activity and MDA level. Data are expressed as mean ± SD (n = 5). ##P < 0.01 vs. Control; *P < 0.05, **P < 0.01 vs. DOX alone. Abbreviations: SAA, Salvianolic acid A; DOX, Doxorubicin.
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f2: The effects of SAA on superoxide dismutase (SOD) activity and level of malondialdehyde (MDA) in doxorubicin-induced nephropathy rats.a: SOD activity, b: MDA level. Rats were treated as described in the legend of Fig. 1. At the end of the experiment, rats were sacrificed and renal cortical tissue were collected for determination of SOD activity and MDA level. Data are expressed as mean ± SD (n = 5). ##P < 0.01 vs. Control; *P < 0.05, **P < 0.01 vs. DOX alone. Abbreviations: SAA, Salvianolic acid A; DOX, Doxorubicin.

Mentions: It was demonstrated that SOD activity was lower in DOX-only rats than that in control; meanwhile, increased MDA level was found in nephropathy rats. SAA (2.5, 5, 10 mg/kg) increased SOD activity while decreasing MDA level. The effect was significant at doses of 5 and 10 mg/kg for SOD activity (P = 0.026 and P = 0.002, respectively), and 10 mg/kg for MDA level (P = 0.009). Prednisone acetate had no notable effect on SOD activity and MDA level (P = 0.796 and P = 0.117, respectively) (Fig. 2a,b).


Salvianolic acid A as a multifunctional agent ameliorates doxorubicin-induced nephropathy in rats.

Fan HY, Yang MY, Qi D, Zhang ZK, Zhu L, Shang-Guan XX, Liu K, Xu H, Che X - Sci Rep (2015)

The effects of SAA on superoxide dismutase (SOD) activity and level of malondialdehyde (MDA) in doxorubicin-induced nephropathy rats.a: SOD activity, b: MDA level. Rats were treated as described in the legend of Fig. 1. At the end of the experiment, rats were sacrificed and renal cortical tissue were collected for determination of SOD activity and MDA level. Data are expressed as mean ± SD (n = 5). ##P < 0.01 vs. Control; *P < 0.05, **P < 0.01 vs. DOX alone. Abbreviations: SAA, Salvianolic acid A; DOX, Doxorubicin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508635&req=5

f2: The effects of SAA on superoxide dismutase (SOD) activity and level of malondialdehyde (MDA) in doxorubicin-induced nephropathy rats.a: SOD activity, b: MDA level. Rats were treated as described in the legend of Fig. 1. At the end of the experiment, rats were sacrificed and renal cortical tissue were collected for determination of SOD activity and MDA level. Data are expressed as mean ± SD (n = 5). ##P < 0.01 vs. Control; *P < 0.05, **P < 0.01 vs. DOX alone. Abbreviations: SAA, Salvianolic acid A; DOX, Doxorubicin.
Mentions: It was demonstrated that SOD activity was lower in DOX-only rats than that in control; meanwhile, increased MDA level was found in nephropathy rats. SAA (2.5, 5, 10 mg/kg) increased SOD activity while decreasing MDA level. The effect was significant at doses of 5 and 10 mg/kg for SOD activity (P = 0.026 and P = 0.002, respectively), and 10 mg/kg for MDA level (P = 0.009). Prednisone acetate had no notable effect on SOD activity and MDA level (P = 0.796 and P = 0.117, respectively) (Fig. 2a,b).

Bottom Line: Evidence suggest that multidrug therapy has more effect than monotherapy in amelioration of renal injury.Results revealed that treatment with SAA alleviated histological damages, relieved proteinuria, hypoalbuminemia and hyperlipidemia, reduced oxidative stress, as well as improving hemorheology.The anti-inflammation, antioxidant, amelioration of podocyte injury, improvement of hemorheology and hypolipidemic properties may constituent an important part of its therapeutic effects.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Yantai University, 264005 Yantai, Shandong Province, China.

ABSTRACT
Nephrotic syndrome (NS) is still a therapeutic challenge. To date there is no ideal treatment. Evidence suggest that multidrug therapy has more effect than monotherapy in amelioration of renal injury. Salvianolic acid A (SAA) is the major active component of Salviae Miltiorrhizae Bunge. Previous studies have demonstrated that SAA is a multi-target agent and has various pharmacological activities. The pleiotropic properties of SAA predict its potential in the treatment of NS. The study investigated the effect of SAA on doxorubicin-induced nephropathy. The kidney function related-biochemical changes, hemorheological parameters and oxidative stress status were determined, and histological examination using light and transmission electron microcopies and western blot analysis were also performed. Results revealed that treatment with SAA alleviated histological damages, relieved proteinuria, hypoalbuminemia and hyperlipidemia, reduced oxidative stress, as well as improving hemorheology. Furthermore, SAA restored podocin expression, down-regulated the expression of NF-κB p65 and p-IκBα while up-regulating IκBα protein expression. Overall, as a multifunctional agent, SAA has a favorable renoprotection in doxorubicin-induced nephropathy. The anti-inflammation, antioxidant, amelioration of podocyte injury, improvement of hemorheology and hypolipidemic properties may constituent an important part of its therapeutic effects. All these indicate that SAA is likely to be a promising agent for NS.

No MeSH data available.


Related in: MedlinePlus