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Escherichia coli of sequence type 3835 carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12.

Feng Y, Yang P, Xie Y, Wang X, McNally A, Zong Z - Sci Rep (2015)

Bottom Line: It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. bla NDM-1 was the only carbapenemase gene detected.In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12 was identified.IncX3 plasmids may be making a significant contribution to the dissemination of bla NDM among Enterobacteriaceae in China.

View Article: PubMed Central - PubMed

Affiliation: 1] Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China [2] Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China.

ABSTRACT
New Delhi metallo-β-lactamase (NDM) represents a serious challenge for treatment and public health. A carbapenem-resistant Escherichia coli clinical strain WCHEC13-8 was subjected to antimicrobial susceptibility tests, whole genome sequencing and conjugation experiments. It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. bla NDM-1 was the only carbapenemase gene detected. Strain WCHEC13-8 also had a plasmid-borne AmpC gene (bla CMY-42) and two extended-spectrum β-lactamase genes (bla CTX-M-15 and bla SHV-12). bla NDM-1 and bla SHV-12 were carried by a 54-kb IncX3 self-transmissible plasmid, which is identical to plasmid pNDM-HF727 from Enterobacter cloacae. bla CMY-42 was carried by a 64-kb IncI1 plasmid and bla CTX-M-15 was located on a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1). bla CMY-42 was in a complicated context and the mobilisation of bla CMY-42 was due to the transposition of ISEcp1 by misidentifying its right-end boundary. Genetic context of bla NDM-1 in strain WCHEC13-8 was closely related to those on IncX3 plasmids in various Enterobacteriaceae species in China. In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12 was identified. IncX3 plasmids may be making a significant contribution to the dissemination of bla NDM among Enterobacteriaceae in China.

No MeSH data available.


Related in: MedlinePlus

Genetic context of blaNDM-1 in E. coli strain WCHEC13-8.ISAba125 was interrupted by the insertion of IS5. Genes shown from the left in order are mpr (encoding a zinc metalloproteinase), blaNDM-1, ble (mediating bleomycin resistance), trpF (encoding a phosphoribosylanthranilate isomerase), dsbC (encoding an oxidoreductase), ctuA1 (encoding an ion tolerant protein), groL (encoding a chaperonin subunit), ygbI (a putative dehydrogenase gene), a truncated ygbJ (a putative DEOR-type transcriptional regulator gene), blaSHV-12 and a truncated umuD (encoding a mutagenesis protein).
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f3: Genetic context of blaNDM-1 in E. coli strain WCHEC13-8.ISAba125 was interrupted by the insertion of IS5. Genes shown from the left in order are mpr (encoding a zinc metalloproteinase), blaNDM-1, ble (mediating bleomycin resistance), trpF (encoding a phosphoribosylanthranilate isomerase), dsbC (encoding an oxidoreductase), ctuA1 (encoding an ion tolerant protein), groL (encoding a chaperonin subunit), ygbI (a putative dehydrogenase gene), a truncated ygbJ (a putative DEOR-type transcriptional regulator gene), blaSHV-12 and a truncated umuD (encoding a mutagenesis protein).

Mentions: The genetic context of blaNDM-1 on pNDM1_EC8 is identical to those on pNDM-HF727 and several other IncX3 plasmids from China. In such a context, blaNDM-1 is adjacent to an IS5-interrupted IS3000-truncated ISAba125 at upstream and is linked with blaSHV-12 at downstream (Fig. 3). blaSHV-12 is flanked by IS26, which could form a composite transposon with the potential to mobilise blaSHV-12 into different locations.


Escherichia coli of sequence type 3835 carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12.

Feng Y, Yang P, Xie Y, Wang X, McNally A, Zong Z - Sci Rep (2015)

Genetic context of blaNDM-1 in E. coli strain WCHEC13-8.ISAba125 was interrupted by the insertion of IS5. Genes shown from the left in order are mpr (encoding a zinc metalloproteinase), blaNDM-1, ble (mediating bleomycin resistance), trpF (encoding a phosphoribosylanthranilate isomerase), dsbC (encoding an oxidoreductase), ctuA1 (encoding an ion tolerant protein), groL (encoding a chaperonin subunit), ygbI (a putative dehydrogenase gene), a truncated ygbJ (a putative DEOR-type transcriptional regulator gene), blaSHV-12 and a truncated umuD (encoding a mutagenesis protein).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508618&req=5

f3: Genetic context of blaNDM-1 in E. coli strain WCHEC13-8.ISAba125 was interrupted by the insertion of IS5. Genes shown from the left in order are mpr (encoding a zinc metalloproteinase), blaNDM-1, ble (mediating bleomycin resistance), trpF (encoding a phosphoribosylanthranilate isomerase), dsbC (encoding an oxidoreductase), ctuA1 (encoding an ion tolerant protein), groL (encoding a chaperonin subunit), ygbI (a putative dehydrogenase gene), a truncated ygbJ (a putative DEOR-type transcriptional regulator gene), blaSHV-12 and a truncated umuD (encoding a mutagenesis protein).
Mentions: The genetic context of blaNDM-1 on pNDM1_EC8 is identical to those on pNDM-HF727 and several other IncX3 plasmids from China. In such a context, blaNDM-1 is adjacent to an IS5-interrupted IS3000-truncated ISAba125 at upstream and is linked with blaSHV-12 at downstream (Fig. 3). blaSHV-12 is flanked by IS26, which could form a composite transposon with the potential to mobilise blaSHV-12 into different locations.

Bottom Line: It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. bla NDM-1 was the only carbapenemase gene detected.In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12 was identified.IncX3 plasmids may be making a significant contribution to the dissemination of bla NDM among Enterobacteriaceae in China.

View Article: PubMed Central - PubMed

Affiliation: 1] Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China [2] Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China.

ABSTRACT
New Delhi metallo-β-lactamase (NDM) represents a serious challenge for treatment and public health. A carbapenem-resistant Escherichia coli clinical strain WCHEC13-8 was subjected to antimicrobial susceptibility tests, whole genome sequencing and conjugation experiments. It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. bla NDM-1 was the only carbapenemase gene detected. Strain WCHEC13-8 also had a plasmid-borne AmpC gene (bla CMY-42) and two extended-spectrum β-lactamase genes (bla CTX-M-15 and bla SHV-12). bla NDM-1 and bla SHV-12 were carried by a 54-kb IncX3 self-transmissible plasmid, which is identical to plasmid pNDM-HF727 from Enterobacter cloacae. bla CMY-42 was carried by a 64-kb IncI1 plasmid and bla CTX-M-15 was located on a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1). bla CMY-42 was in a complicated context and the mobilisation of bla CMY-42 was due to the transposition of ISEcp1 by misidentifying its right-end boundary. Genetic context of bla NDM-1 in strain WCHEC13-8 was closely related to those on IncX3 plasmids in various Enterobacteriaceae species in China. In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12 was identified. IncX3 plasmids may be making a significant contribution to the dissemination of bla NDM among Enterobacteriaceae in China.

No MeSH data available.


Related in: MedlinePlus