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Escherichia coli of sequence type 3835 carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12.

Feng Y, Yang P, Xie Y, Wang X, McNally A, Zong Z - Sci Rep (2015)

Bottom Line: It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. bla NDM-1 was the only carbapenemase gene detected.In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12 was identified.IncX3 plasmids may be making a significant contribution to the dissemination of bla NDM among Enterobacteriaceae in China.

View Article: PubMed Central - PubMed

Affiliation: 1] Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China [2] Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China.

ABSTRACT
New Delhi metallo-β-lactamase (NDM) represents a serious challenge for treatment and public health. A carbapenem-resistant Escherichia coli clinical strain WCHEC13-8 was subjected to antimicrobial susceptibility tests, whole genome sequencing and conjugation experiments. It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. bla NDM-1 was the only carbapenemase gene detected. Strain WCHEC13-8 also had a plasmid-borne AmpC gene (bla CMY-42) and two extended-spectrum β-lactamase genes (bla CTX-M-15 and bla SHV-12). bla NDM-1 and bla SHV-12 were carried by a 54-kb IncX3 self-transmissible plasmid, which is identical to plasmid pNDM-HF727 from Enterobacter cloacae. bla CMY-42 was carried by a 64-kb IncI1 plasmid and bla CTX-M-15 was located on a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1). bla CMY-42 was in a complicated context and the mobilisation of bla CMY-42 was due to the transposition of ISEcp1 by misidentifying its right-end boundary. Genetic context of bla NDM-1 in strain WCHEC13-8 was closely related to those on IncX3 plasmids in various Enterobacteriaceae species in China. In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12 was identified. IncX3 plasmids may be making a significant contribution to the dissemination of bla NDM among Enterobacteriaceae in China.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic trees of IncX3 plasmids.The trees are generated using MEGA based on the complete sequence of IncX3 plasmids. Panel A, a tree of IncX3 plasmids including pKP13d, which carries blaKPC-2 in a K. penumoniae strain from Brazil (GenBank accession number CP003997). pKP13d is distinct from other IncX3 plasmids in sequence. Panel B, a higher resolution tree of IncX3 plasmids excluding pKP13d. The carbapenemase and ESBL genes, the host species, the locations of recovery and the GenBank accession number of each plasmid are shown. For host species, CF refers to Citrobacter freundii, EC to E. coli, ECL to E. cloacae, KP to K. pneumoniae and RP to Raoultella planticola. The plasmid carrying blaNDM-1 identified in this study is identical to pNDM-HF727, which is underlined. The GenBank accession number of pNDM5_021517 is not available.
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f2: Phylogenetic trees of IncX3 plasmids.The trees are generated using MEGA based on the complete sequence of IncX3 plasmids. Panel A, a tree of IncX3 plasmids including pKP13d, which carries blaKPC-2 in a K. penumoniae strain from Brazil (GenBank accession number CP003997). pKP13d is distinct from other IncX3 plasmids in sequence. Panel B, a higher resolution tree of IncX3 plasmids excluding pKP13d. The carbapenemase and ESBL genes, the host species, the locations of recovery and the GenBank accession number of each plasmid are shown. For host species, CF refers to Citrobacter freundii, EC to E. coli, ECL to E. cloacae, KP to K. pneumoniae and RP to Raoultella planticola. The plasmid carrying blaNDM-1 identified in this study is identical to pNDM-HF727, which is underlined. The GenBank accession number of pNDM5_021517 is not available.

Mentions: IncX3 plasmids are narrow-host-range plasmids of Enterobacteriaceae16. A few IncX3 plasmids carrying ESBL genes (blaSHV-11 or blaSHV-12) and/or carbapenemase genes (blaKPC-2, blaNDM-1 or blaNDM-5) from various species of Enterobacteriaceae in several countries have been completely sequenced91718192021. In particular, IncX3 plasmids carrying blaNDM-1 and blaSHV-12 have been identified in various species of the Enterobacteriaceae at multiple locations in China. The completely-sequenced IncX3 plasmids except pKP13d are almost identical in their backbone sequences with less than 9 single nucleotide variations between any two plasmids (Fig. 2). The remarkable similarity between IncX3 plasmids suggests that a common IncX3 plasmid has spread to different continents, acquired the carbapenemase gene that is prevalent locally, e.g. blaKPC-2 in USA and blaNDM-1 in China, and then were transformed as a vehicle mediating the interspecies spread of carbapenemase genes in local settings.


Escherichia coli of sequence type 3835 carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12.

Feng Y, Yang P, Xie Y, Wang X, McNally A, Zong Z - Sci Rep (2015)

Phylogenetic trees of IncX3 plasmids.The trees are generated using MEGA based on the complete sequence of IncX3 plasmids. Panel A, a tree of IncX3 plasmids including pKP13d, which carries blaKPC-2 in a K. penumoniae strain from Brazil (GenBank accession number CP003997). pKP13d is distinct from other IncX3 plasmids in sequence. Panel B, a higher resolution tree of IncX3 plasmids excluding pKP13d. The carbapenemase and ESBL genes, the host species, the locations of recovery and the GenBank accession number of each plasmid are shown. For host species, CF refers to Citrobacter freundii, EC to E. coli, ECL to E. cloacae, KP to K. pneumoniae and RP to Raoultella planticola. The plasmid carrying blaNDM-1 identified in this study is identical to pNDM-HF727, which is underlined. The GenBank accession number of pNDM5_021517 is not available.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508618&req=5

f2: Phylogenetic trees of IncX3 plasmids.The trees are generated using MEGA based on the complete sequence of IncX3 plasmids. Panel A, a tree of IncX3 plasmids including pKP13d, which carries blaKPC-2 in a K. penumoniae strain from Brazil (GenBank accession number CP003997). pKP13d is distinct from other IncX3 plasmids in sequence. Panel B, a higher resolution tree of IncX3 plasmids excluding pKP13d. The carbapenemase and ESBL genes, the host species, the locations of recovery and the GenBank accession number of each plasmid are shown. For host species, CF refers to Citrobacter freundii, EC to E. coli, ECL to E. cloacae, KP to K. pneumoniae and RP to Raoultella planticola. The plasmid carrying blaNDM-1 identified in this study is identical to pNDM-HF727, which is underlined. The GenBank accession number of pNDM5_021517 is not available.
Mentions: IncX3 plasmids are narrow-host-range plasmids of Enterobacteriaceae16. A few IncX3 plasmids carrying ESBL genes (blaSHV-11 or blaSHV-12) and/or carbapenemase genes (blaKPC-2, blaNDM-1 or blaNDM-5) from various species of Enterobacteriaceae in several countries have been completely sequenced91718192021. In particular, IncX3 plasmids carrying blaNDM-1 and blaSHV-12 have been identified in various species of the Enterobacteriaceae at multiple locations in China. The completely-sequenced IncX3 plasmids except pKP13d are almost identical in their backbone sequences with less than 9 single nucleotide variations between any two plasmids (Fig. 2). The remarkable similarity between IncX3 plasmids suggests that a common IncX3 plasmid has spread to different continents, acquired the carbapenemase gene that is prevalent locally, e.g. blaKPC-2 in USA and blaNDM-1 in China, and then were transformed as a vehicle mediating the interspecies spread of carbapenemase genes in local settings.

Bottom Line: It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. bla NDM-1 was the only carbapenemase gene detected.In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12 was identified.IncX3 plasmids may be making a significant contribution to the dissemination of bla NDM among Enterobacteriaceae in China.

View Article: PubMed Central - PubMed

Affiliation: 1] Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China [2] Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China.

ABSTRACT
New Delhi metallo-β-lactamase (NDM) represents a serious challenge for treatment and public health. A carbapenem-resistant Escherichia coli clinical strain WCHEC13-8 was subjected to antimicrobial susceptibility tests, whole genome sequencing and conjugation experiments. It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. bla NDM-1 was the only carbapenemase gene detected. Strain WCHEC13-8 also had a plasmid-borne AmpC gene (bla CMY-42) and two extended-spectrum β-lactamase genes (bla CTX-M-15 and bla SHV-12). bla NDM-1 and bla SHV-12 were carried by a 54-kb IncX3 self-transmissible plasmid, which is identical to plasmid pNDM-HF727 from Enterobacter cloacae. bla CMY-42 was carried by a 64-kb IncI1 plasmid and bla CTX-M-15 was located on a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1). bla CMY-42 was in a complicated context and the mobilisation of bla CMY-42 was due to the transposition of ISEcp1 by misidentifying its right-end boundary. Genetic context of bla NDM-1 in strain WCHEC13-8 was closely related to those on IncX3 plasmids in various Enterobacteriaceae species in China. In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying bla NDM-1, bla CTX-M-15, bla CMY-42 and bla SHV-12 was identified. IncX3 plasmids may be making a significant contribution to the dissemination of bla NDM among Enterobacteriaceae in China.

No MeSH data available.


Related in: MedlinePlus