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Polymerase Chain Reaction as a Diagnostic Tool for Six Sexually Transmitted Infections - Preliminary Results.

Grad AI, Vica ML, Matei HV, Grad DL, Coman I, Tataru DA - Clujul Med (2015)

Bottom Line: We used "Epicenter MasterPure™ Complete DNA and RNA Purification Kit" for the DNA purification and "Seeplex® STD6 ACE Detection" for the DNA amplification.The results were examined in UV light.A number of 5 patients had positive results for Chlamydia trachomatis or Neisseria gonorrhoeae.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, University of Medicine and Pharmacy Iuliu Hatieganu, Cluj Napoca, Romania.

ABSTRACT

Background and aim: Sexually transmitted infections are a very frequent and under-diagnosed cause of illness worldwide. A high number of detection methods and a large range of specimens in which sexually transmitted infections can be determined are available at the moment. Polymerase chain reaction performed on first void urine offers the advantage of being non-invasive, self-collectable and has high sensitivity and specificity. We looked to determine the frequency of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma genitalium and Ureaplasma urealyticum in symptomatic and asymptomatic patients.

Methods: Six sexually transmitted infections were determined in the first void urine of 15 symptomatic and asymptomatic patients by polymerase chain reaction. We used "Epicenter MasterPure™ Complete DNA and RNA Purification Kit" for the DNA purification and "Seeplex® STD6 ACE Detection" for the DNA amplification. The results were examined in UV light.

Results: A number of 5 patients had positive results for Chlamydia trachomatis or Neisseria gonorrhoeae. Sexually transmitted infections are more frequent in men between 27 and 40 years old.

Conclusions: Polymerase chain reaction is a good diagnostic tool for sexually transmitted infections because it has a high sensitivity and specificity. Chlamydia trachomatis is the most frequent sexually transmitted infection, followed by Neisseria gonorrhoeae.

No MeSH data available.


Related in: MedlinePlus

Example of PCR result. NC - negative control. 6, 7, 8, 9 - samples. PC - positive control. MK - marker of molecular weight. Sample 6 - positive for CT. Sample 9 - positive for CT and NG.
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f1-cm8801p33: Example of PCR result. NC - negative control. 6, 7, 8, 9 - samples. PC - positive control. MK - marker of molecular weight. Sample 6 - positive for CT. Sample 9 - positive for CT and NG.

Mentions: Therefore, CT was found in 4 patients and NG in 2 patients. One patient had positive result for CT and also for NG (Fig. 1). MH, MG, TV and UU were not found in this 15 patients.


Polymerase Chain Reaction as a Diagnostic Tool for Six Sexually Transmitted Infections - Preliminary Results.

Grad AI, Vica ML, Matei HV, Grad DL, Coman I, Tataru DA - Clujul Med (2015)

Example of PCR result. NC - negative control. 6, 7, 8, 9 - samples. PC - positive control. MK - marker of molecular weight. Sample 6 - positive for CT. Sample 9 - positive for CT and NG.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508611&req=5

f1-cm8801p33: Example of PCR result. NC - negative control. 6, 7, 8, 9 - samples. PC - positive control. MK - marker of molecular weight. Sample 6 - positive for CT. Sample 9 - positive for CT and NG.
Mentions: Therefore, CT was found in 4 patients and NG in 2 patients. One patient had positive result for CT and also for NG (Fig. 1). MH, MG, TV and UU were not found in this 15 patients.

Bottom Line: We used "Epicenter MasterPure™ Complete DNA and RNA Purification Kit" for the DNA purification and "Seeplex® STD6 ACE Detection" for the DNA amplification.The results were examined in UV light.A number of 5 patients had positive results for Chlamydia trachomatis or Neisseria gonorrhoeae.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, University of Medicine and Pharmacy Iuliu Hatieganu, Cluj Napoca, Romania.

ABSTRACT

Background and aim: Sexually transmitted infections are a very frequent and under-diagnosed cause of illness worldwide. A high number of detection methods and a large range of specimens in which sexually transmitted infections can be determined are available at the moment. Polymerase chain reaction performed on first void urine offers the advantage of being non-invasive, self-collectable and has high sensitivity and specificity. We looked to determine the frequency of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma genitalium and Ureaplasma urealyticum in symptomatic and asymptomatic patients.

Methods: Six sexually transmitted infections were determined in the first void urine of 15 symptomatic and asymptomatic patients by polymerase chain reaction. We used "Epicenter MasterPure™ Complete DNA and RNA Purification Kit" for the DNA purification and "Seeplex® STD6 ACE Detection" for the DNA amplification. The results were examined in UV light.

Results: A number of 5 patients had positive results for Chlamydia trachomatis or Neisseria gonorrhoeae. Sexually transmitted infections are more frequent in men between 27 and 40 years old.

Conclusions: Polymerase chain reaction is a good diagnostic tool for sexually transmitted infections because it has a high sensitivity and specificity. Chlamydia trachomatis is the most frequent sexually transmitted infection, followed by Neisseria gonorrhoeae.

No MeSH data available.


Related in: MedlinePlus