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Identification of PblB mediating galactose-specific adhesion in a successful Streptococcus pneumoniae clone.

Hsieh YC, Lin TL, Lin CM, Wang JT - Sci Rep (2015)

Bottom Line: Preincubation of NTUH-P15 with D-galactose resulted in decreases of adherence to A549 cell in a dose-dependent manner.Challenge of mice with NTUH-P15, isogenic pblB mutant and pblB complementation strains determined that PblB was required for bacterial persistence in the nasopharynx and lung.PblB, as an adhesin mediating the galactose-specific adhesion activity of pneumococci, promote pneumococcal clonal success.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Chang Gung Children's Hospital, Chang Gung Memorial Hospital, Chang Gung University, College of Medicine, Taoyuan, Taiwan.

ABSTRACT
The pneumococcal genome is variable and there are minimal data on the influence of the accessory genome on phenotype. Pneumococcal serotype 14 sequence type (ST) 46 had been the most prevalent clone causing pneumonia in children in Taiwan. A microarray was constructed using the genomic DNA of a clinical strain (NTUH-P15) of serotype 14 ST46. Using DNA hybridization, genomic variations in NTUH-P15 were compared to those of 3 control strains. Microarray analysis identified 7 genomic regions that had significant increases in hybridization signals in the NTUH-P15 strain compared to control strains. One of these regions encoded PblB, a phage-encoded virulence factor implicated (in Streptococcus mitis) in infective endocarditis. The isogenic pblB mutant decreased adherence to A549 human lung epithelial cell compared to wild-type NTUH-P15 strain (P = 0.01). Complementation with pblB restored the adherence. PblB is predicted to contain a galactose-binding domain-like region. Preincubation of NTUH-P15 with D-galactose resulted in decreases of adherence to A549 cell in a dose-dependent manner. Challenge of mice with NTUH-P15, isogenic pblB mutant and pblB complementation strains determined that PblB was required for bacterial persistence in the nasopharynx and lung. PblB, as an adhesin mediating the galactose-specific adhesion activity of pneumococci, promote pneumococcal clonal success.

No MeSH data available.


Related in: MedlinePlus

DNA hybridization and colorimetric detection of NTUH-P15 and 3 non-clonal expansion strains on microarray analysis.Each spot represents one clone. The spot showing significant increases in hybridization signals in the NTUH-P15 strain compared with the other three strains are circled. Spots showing no significant increases in hybridization signals in the NTUH-P15 strain compared with the other three strains are not circled.
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f1: DNA hybridization and colorimetric detection of NTUH-P15 and 3 non-clonal expansion strains on microarray analysis.Each spot represents one clone. The spot showing significant increases in hybridization signals in the NTUH-P15 strain compared with the other three strains are circled. Spots showing no significant increases in hybridization signals in the NTUH-P15 strain compared with the other three strains are not circled.

Mentions: Comparison of microarray results for NTUH-P15 and 3 control strains (NTUH-P3, CGCH1 and CGCH2) revealed 7 “spots” (plasmid clones) that had significantly higher hybridization signals (defined as >10-fold differences) in the NTUH-P15 strain. The inserts of these 7 plasmid clones were sequenced and subjected to sequence similarity (BLAST) searches. Genes contained in each clone are shown in Table 1. Blast searches of clone 1 identified that clone 1 contained genes with similarity to the adjacent loci SPP_0074 and SPP_0075 of S. pneumoniae P1031 (Table 1); the products of these genes exhibit similarity to a host specificity protein and PblB, respectively (Fig. 1). From literature review, PblB of Streptococcus mitis, a phage-encoded virulence factor, was implicated in infective endocarditis10. Therefore, we chose plasmid clone 1 for further study.


Identification of PblB mediating galactose-specific adhesion in a successful Streptococcus pneumoniae clone.

Hsieh YC, Lin TL, Lin CM, Wang JT - Sci Rep (2015)

DNA hybridization and colorimetric detection of NTUH-P15 and 3 non-clonal expansion strains on microarray analysis.Each spot represents one clone. The spot showing significant increases in hybridization signals in the NTUH-P15 strain compared with the other three strains are circled. Spots showing no significant increases in hybridization signals in the NTUH-P15 strain compared with the other three strains are not circled.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508584&req=5

f1: DNA hybridization and colorimetric detection of NTUH-P15 and 3 non-clonal expansion strains on microarray analysis.Each spot represents one clone. The spot showing significant increases in hybridization signals in the NTUH-P15 strain compared with the other three strains are circled. Spots showing no significant increases in hybridization signals in the NTUH-P15 strain compared with the other three strains are not circled.
Mentions: Comparison of microarray results for NTUH-P15 and 3 control strains (NTUH-P3, CGCH1 and CGCH2) revealed 7 “spots” (plasmid clones) that had significantly higher hybridization signals (defined as >10-fold differences) in the NTUH-P15 strain. The inserts of these 7 plasmid clones were sequenced and subjected to sequence similarity (BLAST) searches. Genes contained in each clone are shown in Table 1. Blast searches of clone 1 identified that clone 1 contained genes with similarity to the adjacent loci SPP_0074 and SPP_0075 of S. pneumoniae P1031 (Table 1); the products of these genes exhibit similarity to a host specificity protein and PblB, respectively (Fig. 1). From literature review, PblB of Streptococcus mitis, a phage-encoded virulence factor, was implicated in infective endocarditis10. Therefore, we chose plasmid clone 1 for further study.

Bottom Line: Preincubation of NTUH-P15 with D-galactose resulted in decreases of adherence to A549 cell in a dose-dependent manner.Challenge of mice with NTUH-P15, isogenic pblB mutant and pblB complementation strains determined that PblB was required for bacterial persistence in the nasopharynx and lung.PblB, as an adhesin mediating the galactose-specific adhesion activity of pneumococci, promote pneumococcal clonal success.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Chang Gung Children's Hospital, Chang Gung Memorial Hospital, Chang Gung University, College of Medicine, Taoyuan, Taiwan.

ABSTRACT
The pneumococcal genome is variable and there are minimal data on the influence of the accessory genome on phenotype. Pneumococcal serotype 14 sequence type (ST) 46 had been the most prevalent clone causing pneumonia in children in Taiwan. A microarray was constructed using the genomic DNA of a clinical strain (NTUH-P15) of serotype 14 ST46. Using DNA hybridization, genomic variations in NTUH-P15 were compared to those of 3 control strains. Microarray analysis identified 7 genomic regions that had significant increases in hybridization signals in the NTUH-P15 strain compared to control strains. One of these regions encoded PblB, a phage-encoded virulence factor implicated (in Streptococcus mitis) in infective endocarditis. The isogenic pblB mutant decreased adherence to A549 human lung epithelial cell compared to wild-type NTUH-P15 strain (P = 0.01). Complementation with pblB restored the adherence. PblB is predicted to contain a galactose-binding domain-like region. Preincubation of NTUH-P15 with D-galactose resulted in decreases of adherence to A549 cell in a dose-dependent manner. Challenge of mice with NTUH-P15, isogenic pblB mutant and pblB complementation strains determined that PblB was required for bacterial persistence in the nasopharynx and lung. PblB, as an adhesin mediating the galactose-specific adhesion activity of pneumococci, promote pneumococcal clonal success.

No MeSH data available.


Related in: MedlinePlus