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Apoptosis-Inducing Activity of Marine Sponge Haliclona sp. Extracts Collected from Kosrae in Nonsmall Cell Lung Cancer A549 Cells.

Bae W, Lim HK, Kim KM, Cho H, Lee SY, Jeong CS, Lee HS, Jung J - Evid Based Complement Alternat Med (2015)

Bottom Line: Further, methanol extracts of Haliclona sp. significantly inhibited cell proliferation and cell viability.A549 cells treated with Haliclona sp. demonstrated induced expression of c-Jun N-terminal kinase (JNK), p53, p21, caspase-8, and caspase-3.The percentage of apoptotic cells significantly increased in A549 cultures treated with Haliclona sp.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Duksung Women's University, Seoul 132-714, Republic of Korea.

ABSTRACT
Although various anticancer drugs have been developed for the treatment of nonsmall cell lung cancer, chemotherapeutic efficacy is still limited. Natural products such as phytochemicals have been screened as novel alternative materials, but alternative funds such as marine bioresources remain largely untapped. Of these resources, marine sponges have undergone the most scrutiny for their biological activities, including antiinflammatory, antiviral, and anticancer properties. However, the biological mechanisms of the activities of these marine sponges are still unclear. We investigated the anticancer activity of marine sponges collected from Kosrae in Micronesia and examined their mechanisms of action using nonsmall cell lung cancer A549 cells as a model system. Of 20 specimens, the Haliclona sp. (KO1304-328) showed both dose- and time-dependent cytotoxicity. Further, methanol extracts of Haliclona sp. significantly inhibited cell proliferation and cell viability. A549 cells treated with Haliclona sp. demonstrated induced expression of c-Jun N-terminal kinase (JNK), p53, p21, caspase-8, and caspase-3. The percentage of apoptotic cells significantly increased in A549 cultures treated with Haliclona sp. These results indicate that Haliclona sp. induces apoptosis via the JNK-p53 pathway and caspase-8, suggesting that this marine sponge is a good resource for the development of drugs for treatment of nonsmall cell lung cancer.

No MeSH data available.


Related in: MedlinePlus

Morphology of Haliclona sp.
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Related In: Results  -  Collection


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fig1: Morphology of Haliclona sp.

Mentions: The cytotoxicity of 20 sponge specimens (KO1304 series) was determined in order to determine their use as an anticancer drug candidate resource. Each specimen was serially diluted and applied to A549 cells for 48 h. As shown in Table 1, despite the inactivity of the other specimens, KO1304-328 inhibited cell viability by more than 30%. KO1304-328 was identified as Haliclona sp. (Figure 1), which has been reported to have cytotoxic [9], antibacterial [10, 11], antifungal [11], and anticancer effects in breast, prostate, and colon cancer cells [9, 12]. Papuamine and haliclonadiamine isolated from Haliclona sp. were reported as active components [12]. However, the anticancer mechanism of Haliclona sp. is unclear, particularly that against human nonsmall cell lung cancer. Haliclona sp. extract is widely described in complementary medicine, although the bioactive constituents of Haliclona sp. had not been yet isolated and evaluated. Therefore, the mechanism of the anticancer activity of Haliclona sp. extract was investigated in human nonsmall cell lung cancer A549 cells.


Apoptosis-Inducing Activity of Marine Sponge Haliclona sp. Extracts Collected from Kosrae in Nonsmall Cell Lung Cancer A549 Cells.

Bae W, Lim HK, Kim KM, Cho H, Lee SY, Jeong CS, Lee HS, Jung J - Evid Based Complement Alternat Med (2015)

Morphology of Haliclona sp.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508479&req=5

fig1: Morphology of Haliclona sp.
Mentions: The cytotoxicity of 20 sponge specimens (KO1304 series) was determined in order to determine their use as an anticancer drug candidate resource. Each specimen was serially diluted and applied to A549 cells for 48 h. As shown in Table 1, despite the inactivity of the other specimens, KO1304-328 inhibited cell viability by more than 30%. KO1304-328 was identified as Haliclona sp. (Figure 1), which has been reported to have cytotoxic [9], antibacterial [10, 11], antifungal [11], and anticancer effects in breast, prostate, and colon cancer cells [9, 12]. Papuamine and haliclonadiamine isolated from Haliclona sp. were reported as active components [12]. However, the anticancer mechanism of Haliclona sp. is unclear, particularly that against human nonsmall cell lung cancer. Haliclona sp. extract is widely described in complementary medicine, although the bioactive constituents of Haliclona sp. had not been yet isolated and evaluated. Therefore, the mechanism of the anticancer activity of Haliclona sp. extract was investigated in human nonsmall cell lung cancer A549 cells.

Bottom Line: Further, methanol extracts of Haliclona sp. significantly inhibited cell proliferation and cell viability.A549 cells treated with Haliclona sp. demonstrated induced expression of c-Jun N-terminal kinase (JNK), p53, p21, caspase-8, and caspase-3.The percentage of apoptotic cells significantly increased in A549 cultures treated with Haliclona sp.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Duksung Women's University, Seoul 132-714, Republic of Korea.

ABSTRACT
Although various anticancer drugs have been developed for the treatment of nonsmall cell lung cancer, chemotherapeutic efficacy is still limited. Natural products such as phytochemicals have been screened as novel alternative materials, but alternative funds such as marine bioresources remain largely untapped. Of these resources, marine sponges have undergone the most scrutiny for their biological activities, including antiinflammatory, antiviral, and anticancer properties. However, the biological mechanisms of the activities of these marine sponges are still unclear. We investigated the anticancer activity of marine sponges collected from Kosrae in Micronesia and examined their mechanisms of action using nonsmall cell lung cancer A549 cells as a model system. Of 20 specimens, the Haliclona sp. (KO1304-328) showed both dose- and time-dependent cytotoxicity. Further, methanol extracts of Haliclona sp. significantly inhibited cell proliferation and cell viability. A549 cells treated with Haliclona sp. demonstrated induced expression of c-Jun N-terminal kinase (JNK), p53, p21, caspase-8, and caspase-3. The percentage of apoptotic cells significantly increased in A549 cultures treated with Haliclona sp. These results indicate that Haliclona sp. induces apoptosis via the JNK-p53 pathway and caspase-8, suggesting that this marine sponge is a good resource for the development of drugs for treatment of nonsmall cell lung cancer.

No MeSH data available.


Related in: MedlinePlus