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Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma.

de Souza MV, Silva MB, Pinto Jde O, Lima MB, Crepaldi J, Lopes GF, dos Santos HB, Ribeiro RI, Thomé RG - Biomed Res Int (2015)

Bottom Line: The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively.The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III.However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.

ABSTRACT
This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

No MeSH data available.


Related in: MedlinePlus

Percentage of immunostained area for collagen types III (a) and I (b) in normal skin, as well as in the treated and control groups as a function of time: on the day of wound making (T0), at 14 days (T1) of the healing process, and after complete wound closure (T2). Means followed by different lowercase and uppercase letters denote by t-test, respectively, differences in each group evaluated at T1 and T2 relative to T0, and between groups within each time (T1 and T2).
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fig5: Percentage of immunostained area for collagen types III (a) and I (b) in normal skin, as well as in the treated and control groups as a function of time: on the day of wound making (T0), at 14 days (T1) of the healing process, and after complete wound closure (T2). Means followed by different lowercase and uppercase letters denote by t-test, respectively, differences in each group evaluated at T1 and T2 relative to T0, and between groups within each time (T1 and T2).

Mentions: The immunostained area of COL III in the intact skin (T0) was 3.92 ± 0.61%. At 14 days of wound creation the COL III of the treated group showed a smaller immunostained area (1.96 ± 0.32%) as compared with T0 (p < 0.05). However, at 37 days, immunostaining of COL III (3.00 ± 0.58%) was not statistically different in wounds that received LP-PRP relative to T0. Wounds not treated with LP-PRP at 14 days (2.97 ± 0.27%) and 37 days (2.64 ± 0.61%) did not differ from T0. In T1, the area immunostained for COL III in the treated group was smaller (p < 0.05) than control. In T2, the values achieved in the immunostained area of the treated animals were higher than in control (Figure 5(a)).


Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma.

de Souza MV, Silva MB, Pinto Jde O, Lima MB, Crepaldi J, Lopes GF, dos Santos HB, Ribeiro RI, Thomé RG - Biomed Res Int (2015)

Percentage of immunostained area for collagen types III (a) and I (b) in normal skin, as well as in the treated and control groups as a function of time: on the day of wound making (T0), at 14 days (T1) of the healing process, and after complete wound closure (T2). Means followed by different lowercase and uppercase letters denote by t-test, respectively, differences in each group evaluated at T1 and T2 relative to T0, and between groups within each time (T1 and T2).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4508476&req=5

fig5: Percentage of immunostained area for collagen types III (a) and I (b) in normal skin, as well as in the treated and control groups as a function of time: on the day of wound making (T0), at 14 days (T1) of the healing process, and after complete wound closure (T2). Means followed by different lowercase and uppercase letters denote by t-test, respectively, differences in each group evaluated at T1 and T2 relative to T0, and between groups within each time (T1 and T2).
Mentions: The immunostained area of COL III in the intact skin (T0) was 3.92 ± 0.61%. At 14 days of wound creation the COL III of the treated group showed a smaller immunostained area (1.96 ± 0.32%) as compared with T0 (p < 0.05). However, at 37 days, immunostaining of COL III (3.00 ± 0.58%) was not statistically different in wounds that received LP-PRP relative to T0. Wounds not treated with LP-PRP at 14 days (2.97 ± 0.27%) and 37 days (2.64 ± 0.61%) did not differ from T0. In T1, the area immunostained for COL III in the treated group was smaller (p < 0.05) than control. In T2, the values achieved in the immunostained area of the treated animals were higher than in control (Figure 5(a)).

Bottom Line: The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively.The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III.However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.

ABSTRACT
This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

No MeSH data available.


Related in: MedlinePlus