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Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma.

de Souza MV, Silva MB, Pinto Jde O, Lima MB, Crepaldi J, Lopes GF, dos Santos HB, Ribeiro RI, Thomé RG - Biomed Res Int (2015)

Bottom Line: The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively.The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III.However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.

ABSTRACT
This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

No MeSH data available.


Related in: MedlinePlus

Photomicrography of skin before (a) and during the healing process (b–d), where (b) is after two weeks, and (c) and (d) after complete wound closure. Stratified squamous epithelial tissue (Ep), papillary dermis (PD), reticular dermis (RD), dilated capillaries (black arrowhead), fibroblasts (white arrowhead), and immune cells (arrows). HE stain. Bars = 50 μm.
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fig2: Photomicrography of skin before (a) and during the healing process (b–d), where (b) is after two weeks, and (c) and (d) after complete wound closure. Stratified squamous epithelial tissue (Ep), papillary dermis (PD), reticular dermis (RD), dilated capillaries (black arrowhead), fibroblasts (white arrowhead), and immune cells (arrows). HE stain. Bars = 50 μm.

Mentions: The images obtained in the histological analysis of the HE-stained skin are shown in Figure 2. At time zero (making of the wound) it was revealed that the skin had a normal character, that is, an epidermis with poorly keratinized, stratified squamous epithelial tissue, and dermis with two well-defined layers: papillary (superficial) and reticular (deep). Annexes hair follicle, hair erector muscle, sebaceous gland, and sweat glands were observed in these layers (Figure 2(a)).


Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma.

de Souza MV, Silva MB, Pinto Jde O, Lima MB, Crepaldi J, Lopes GF, dos Santos HB, Ribeiro RI, Thomé RG - Biomed Res Int (2015)

Photomicrography of skin before (a) and during the healing process (b–d), where (b) is after two weeks, and (c) and (d) after complete wound closure. Stratified squamous epithelial tissue (Ep), papillary dermis (PD), reticular dermis (RD), dilated capillaries (black arrowhead), fibroblasts (white arrowhead), and immune cells (arrows). HE stain. Bars = 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4508476&req=5

fig2: Photomicrography of skin before (a) and during the healing process (b–d), where (b) is after two weeks, and (c) and (d) after complete wound closure. Stratified squamous epithelial tissue (Ep), papillary dermis (PD), reticular dermis (RD), dilated capillaries (black arrowhead), fibroblasts (white arrowhead), and immune cells (arrows). HE stain. Bars = 50 μm.
Mentions: The images obtained in the histological analysis of the HE-stained skin are shown in Figure 2. At time zero (making of the wound) it was revealed that the skin had a normal character, that is, an epidermis with poorly keratinized, stratified squamous epithelial tissue, and dermis with two well-defined layers: papillary (superficial) and reticular (deep). Annexes hair follicle, hair erector muscle, sebaceous gland, and sweat glands were observed in these layers (Figure 2(a)).

Bottom Line: The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively.The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III.However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.

ABSTRACT
This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

No MeSH data available.


Related in: MedlinePlus