Limits...
Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma.

de Souza MV, Silva MB, Pinto Jde O, Lima MB, Crepaldi J, Lopes GF, dos Santos HB, Ribeiro RI, Thomé RG - Biomed Res Int (2015)

Bottom Line: The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively.The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III.However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.

ABSTRACT
This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

No MeSH data available.


Related in: MedlinePlus

Administration of 0.5 mL leukocyte-poor platelet-rich plasma on the wound edges, using a 24-gauge needle (a, b). Skin sample collection using a scalpel and a needle, after cutting with a 6 mm diameter Punch (c). The obtained fragments were immediately placed on histology cassettes (d) and fixed in 10% formalin.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4508476&req=5

fig1: Administration of 0.5 mL leukocyte-poor platelet-rich plasma on the wound edges, using a 24-gauge needle (a, b). Skin sample collection using a scalpel and a needle, after cutting with a 6 mm diameter Punch (c). The obtained fragments were immediately placed on histology cassettes (d) and fixed in 10% formalin.

Mentions: The platelet-rich plasma was prepared immediately before its administration, which was performed using a 24-gauge needle. Each edge of the two wounds (A and B) received 0.5 mL of the LP-PRP (Figures 1(a)-1(b)), totaling 2 mL per wound and 4 mL per side (left or right) of the gluteal region. After the treatment, the animals were kept in stalls and let out for a daily period of 2 h and monitored throughout the experiment.


Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma.

de Souza MV, Silva MB, Pinto Jde O, Lima MB, Crepaldi J, Lopes GF, dos Santos HB, Ribeiro RI, Thomé RG - Biomed Res Int (2015)

Administration of 0.5 mL leukocyte-poor platelet-rich plasma on the wound edges, using a 24-gauge needle (a, b). Skin sample collection using a scalpel and a needle, after cutting with a 6 mm diameter Punch (c). The obtained fragments were immediately placed on histology cassettes (d) and fixed in 10% formalin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508476&req=5

fig1: Administration of 0.5 mL leukocyte-poor platelet-rich plasma on the wound edges, using a 24-gauge needle (a, b). Skin sample collection using a scalpel and a needle, after cutting with a 6 mm diameter Punch (c). The obtained fragments were immediately placed on histology cassettes (d) and fixed in 10% formalin.
Mentions: The platelet-rich plasma was prepared immediately before its administration, which was performed using a 24-gauge needle. Each edge of the two wounds (A and B) received 0.5 mL of the LP-PRP (Figures 1(a)-1(b)), totaling 2 mL per wound and 4 mL per side (left or right) of the gluteal region. After the treatment, the animals were kept in stalls and let out for a daily period of 2 h and monitored throughout the experiment.

Bottom Line: The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively.The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III.However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.

ABSTRACT
This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

No MeSH data available.


Related in: MedlinePlus