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Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions.

Mokhtari K, Rufino-Palomares EE, Pérez-Jiménez A, Reyes-Zurita FJ, Figuera C, García-Salguero L, Medina PP, Peragón J, Lupiáñez JA - Evid Based Complement Alternat Med (2015)

Bottom Line: FBS absence reduced cell viability decreasing IC50 values of MA.Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain ; Department of Microbiology, Faculty of Sciences, Mohammed I University of Oujda, 60000 Oujda, Morocco.

ABSTRACT
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

No MeSH data available.


Related in: MedlinePlus

Western blots of CAT (a), G6PDH (b), GST (c), and SOD (d) on B16F10 cells cultivated without FBS at different MA dosages. The levels of specific protein expression are shown as arbitrary intensity units of each band compared to arbitrary intensity units of actin. Values are expressed as means ± SD. Different letters indicate significant differences (P < 0.05).
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fig5: Western blots of CAT (a), G6PDH (b), GST (c), and SOD (d) on B16F10 cells cultivated without FBS at different MA dosages. The levels of specific protein expression are shown as arbitrary intensity units of each band compared to arbitrary intensity units of actin. Values are expressed as means ± SD. Different letters indicate significant differences (P < 0.05).

Mentions: To corroborate the effect of different MA levels in cells without FBS in the antioxidant enzymes measured, an immune-blotting analysis was carried out to confirm the differential expression of same specific enzymes: CAT, G6PDH, GST, and SOD (Figure 5), under the same experimental condition (MA levels without FBS). Results obtained of immune-blotting analysis agree with those in the enzyme analysis, tending to decrease their activity and expression as MA concentration increases without FBS, although this pattern is not statistically significant in all enzymes.


Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions.

Mokhtari K, Rufino-Palomares EE, Pérez-Jiménez A, Reyes-Zurita FJ, Figuera C, García-Salguero L, Medina PP, Peragón J, Lupiáñez JA - Evid Based Complement Alternat Med (2015)

Western blots of CAT (a), G6PDH (b), GST (c), and SOD (d) on B16F10 cells cultivated without FBS at different MA dosages. The levels of specific protein expression are shown as arbitrary intensity units of each band compared to arbitrary intensity units of actin. Values are expressed as means ± SD. Different letters indicate significant differences (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508474&req=5

fig5: Western blots of CAT (a), G6PDH (b), GST (c), and SOD (d) on B16F10 cells cultivated without FBS at different MA dosages. The levels of specific protein expression are shown as arbitrary intensity units of each band compared to arbitrary intensity units of actin. Values are expressed as means ± SD. Different letters indicate significant differences (P < 0.05).
Mentions: To corroborate the effect of different MA levels in cells without FBS in the antioxidant enzymes measured, an immune-blotting analysis was carried out to confirm the differential expression of same specific enzymes: CAT, G6PDH, GST, and SOD (Figure 5), under the same experimental condition (MA levels without FBS). Results obtained of immune-blotting analysis agree with those in the enzyme analysis, tending to decrease their activity and expression as MA concentration increases without FBS, although this pattern is not statistically significant in all enzymes.

Bottom Line: FBS absence reduced cell viability decreasing IC50 values of MA.Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain ; Department of Microbiology, Faculty of Sciences, Mohammed I University of Oujda, 60000 Oujda, Morocco.

ABSTRACT
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

No MeSH data available.


Related in: MedlinePlus