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Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions.

Mokhtari K, Rufino-Palomares EE, Pérez-Jiménez A, Reyes-Zurita FJ, Figuera C, García-Salguero L, Medina PP, Peragón J, Lupiáñez JA - Evid Based Complement Alternat Med (2015)

Bottom Line: FBS absence reduced cell viability decreasing IC50 values of MA.Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain ; Department of Microbiology, Faculty of Sciences, Mohammed I University of Oujda, 60000 Oujda, Morocco.

ABSTRACT
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

No MeSH data available.


Related in: MedlinePlus

CAT (a), G6PDH (b), GST (c), and SOD (d) specific activities on B16F10 cells. On the left side, cells cultivated with and without FBS, and on the right side, cell cultivated without FBS and different MA dosages. Values are expressed as means ± SD. Different letters indicate significant differences (P < 0.05).
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fig4: CAT (a), G6PDH (b), GST (c), and SOD (d) specific activities on B16F10 cells. On the left side, cells cultivated with and without FBS, and on the right side, cell cultivated without FBS and different MA dosages. Values are expressed as means ± SD. Different letters indicate significant differences (P < 0.05).

Mentions: An evaluation of the activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glucose 6-phosphate dehydrogenase (G6PDH), and glutathione-S-transferase (GST) was performed under our experimental conditions (Figure 4). The results indicated that CAT, G6PDH, and SOD showed no change in their activity in the absence of FBS compared to the control with FBS, and only GST activity increased in the absence of FBS with respect to control in the presence of FBS, since this enzyme is related to detoxification of lipid peroxidation products, which are being generated under the assayed conditions.


Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions.

Mokhtari K, Rufino-Palomares EE, Pérez-Jiménez A, Reyes-Zurita FJ, Figuera C, García-Salguero L, Medina PP, Peragón J, Lupiáñez JA - Evid Based Complement Alternat Med (2015)

CAT (a), G6PDH (b), GST (c), and SOD (d) specific activities on B16F10 cells. On the left side, cells cultivated with and without FBS, and on the right side, cell cultivated without FBS and different MA dosages. Values are expressed as means ± SD. Different letters indicate significant differences (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508474&req=5

fig4: CAT (a), G6PDH (b), GST (c), and SOD (d) specific activities on B16F10 cells. On the left side, cells cultivated with and without FBS, and on the right side, cell cultivated without FBS and different MA dosages. Values are expressed as means ± SD. Different letters indicate significant differences (P < 0.05).
Mentions: An evaluation of the activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glucose 6-phosphate dehydrogenase (G6PDH), and glutathione-S-transferase (GST) was performed under our experimental conditions (Figure 4). The results indicated that CAT, G6PDH, and SOD showed no change in their activity in the absence of FBS compared to the control with FBS, and only GST activity increased in the absence of FBS with respect to control in the presence of FBS, since this enzyme is related to detoxification of lipid peroxidation products, which are being generated under the assayed conditions.

Bottom Line: FBS absence reduced cell viability decreasing IC50 values of MA.Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain ; Department of Microbiology, Faculty of Sciences, Mohammed I University of Oujda, 60000 Oujda, Morocco.

ABSTRACT
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

No MeSH data available.


Related in: MedlinePlus