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Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions.

Mokhtari K, Rufino-Palomares EE, Pérez-Jiménez A, Reyes-Zurita FJ, Figuera C, García-Salguero L, Medina PP, Peragón J, Lupiáñez JA - Evid Based Complement Alternat Med (2015)

Bottom Line: FBS absence reduced cell viability decreasing IC50 values of MA.Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain ; Department of Microbiology, Faculty of Sciences, Mohammed I University of Oujda, 60000 Oujda, Morocco.

ABSTRACT
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

No MeSH data available.


Related in: MedlinePlus

Positive fluorescent Rh123 on B16F10 cells with or without FBS (a) and after MA treatment at different dosages without FBS (b).
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fig3: Positive fluorescent Rh123 on B16F10 cells with or without FBS (a) and after MA treatment at different dosages without FBS (b).

Mentions: DHR 123 is a ROS assay in which this compound can be oxidized intracellularly by ROS to form the positively charged fluorescent Rh123 [42] and is accumulated into the mitochondria [43]. We have performed an assay of ROS production by DHR in the absence of FBS and using three different MA levels (IC50/8, IC50/2, and IC50) based on our cytotoxicity results. Firstly, we have shown that the absence of FBS produced an increase in ROS production due to mitochondrial disruption (Figure 3(a)). FBS composition includes proteins, growth factors, hormones, and ions [44, 45]. Cell culture without FBS forces the cell to synthesize all required compounds necessary for its normal metabolic activity. This fact involves an increase in the cellular metabolism which is reflected in a higher cellular mitochondrial activity, indirectly determined by DHR 123 assay as shown in quadrant 4 (Q4).


Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions.

Mokhtari K, Rufino-Palomares EE, Pérez-Jiménez A, Reyes-Zurita FJ, Figuera C, García-Salguero L, Medina PP, Peragón J, Lupiáñez JA - Evid Based Complement Alternat Med (2015)

Positive fluorescent Rh123 on B16F10 cells with or without FBS (a) and after MA treatment at different dosages without FBS (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508474&req=5

fig3: Positive fluorescent Rh123 on B16F10 cells with or without FBS (a) and after MA treatment at different dosages without FBS (b).
Mentions: DHR 123 is a ROS assay in which this compound can be oxidized intracellularly by ROS to form the positively charged fluorescent Rh123 [42] and is accumulated into the mitochondria [43]. We have performed an assay of ROS production by DHR in the absence of FBS and using three different MA levels (IC50/8, IC50/2, and IC50) based on our cytotoxicity results. Firstly, we have shown that the absence of FBS produced an increase in ROS production due to mitochondrial disruption (Figure 3(a)). FBS composition includes proteins, growth factors, hormones, and ions [44, 45]. Cell culture without FBS forces the cell to synthesize all required compounds necessary for its normal metabolic activity. This fact involves an increase in the cellular metabolism which is reflected in a higher cellular mitochondrial activity, indirectly determined by DHR 123 assay as shown in quadrant 4 (Q4).

Bottom Line: FBS absence reduced cell viability decreasing IC50 values of MA.Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain ; Department of Microbiology, Faculty of Sciences, Mohammed I University of Oujda, 60000 Oujda, Morocco.

ABSTRACT
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

No MeSH data available.


Related in: MedlinePlus