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Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions.

Mokhtari K, Rufino-Palomares EE, Pérez-Jiménez A, Reyes-Zurita FJ, Figuera C, García-Salguero L, Medina PP, Peragón J, Lupiáñez JA - Evid Based Complement Alternat Med (2015)

Bottom Line: FBS absence reduced cell viability decreasing IC50 values of MA.Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain ; Department of Microbiology, Faculty of Sciences, Mohammed I University of Oujda, 60000 Oujda, Morocco.

ABSTRACT
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

No MeSH data available.


Related in: MedlinePlus

The effect of MA on B16F10 murine melanoma cell viability in the presence of FBS (a) and in the absence of FBS (b). MA cytotoxic doses are shown in (c). Cell proliferation was determined by MTT assay. Values are expressed as means ± SD.
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fig2: The effect of MA on B16F10 murine melanoma cell viability in the presence of FBS (a) and in the absence of FBS (b). MA cytotoxic doses are shown in (c). Cell proliferation was determined by MTT assay. Values are expressed as means ± SD.

Mentions: Percentage of living cells (viable formazan accumulating cells) decreased in a dose dependent manner in the presence and absence of FBS. We noticed that 50% growth inhibition values (IC50) in response to MA were different in media without FBS, compared to media supplemented with FBS; therefore, MA has an increased cytotoxic effect when cells do not have FBS. These results are reported in Figure 2.


Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions.

Mokhtari K, Rufino-Palomares EE, Pérez-Jiménez A, Reyes-Zurita FJ, Figuera C, García-Salguero L, Medina PP, Peragón J, Lupiáñez JA - Evid Based Complement Alternat Med (2015)

The effect of MA on B16F10 murine melanoma cell viability in the presence of FBS (a) and in the absence of FBS (b). MA cytotoxic doses are shown in (c). Cell proliferation was determined by MTT assay. Values are expressed as means ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508474&req=5

fig2: The effect of MA on B16F10 murine melanoma cell viability in the presence of FBS (a) and in the absence of FBS (b). MA cytotoxic doses are shown in (c). Cell proliferation was determined by MTT assay. Values are expressed as means ± SD.
Mentions: Percentage of living cells (viable formazan accumulating cells) decreased in a dose dependent manner in the presence and absence of FBS. We noticed that 50% growth inhibition values (IC50) in response to MA were different in media without FBS, compared to media supplemented with FBS; therefore, MA has an increased cytotoxic effect when cells do not have FBS. These results are reported in Figure 2.

Bottom Line: FBS absence reduced cell viability decreasing IC50 values of MA.Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain ; Department of Microbiology, Faculty of Sciences, Mohammed I University of Oujda, 60000 Oujda, Morocco.

ABSTRACT
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA. The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense. However, at higher dosages MA induced cellular damage by apoptosis as seen in the results obtained.

No MeSH data available.


Related in: MedlinePlus