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Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans.

Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P - Oxid Med Cell Longev (2015)

Bottom Line: Carqueja (Baccharis trimera) is a native plant found throughout South America.CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes.Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

ABSTRACT
Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activity in vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potential in vivo are limited. In this study, we used Caenorhabditis elegans as a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in a C. elegans model for Alzheimer's disease. Here, we show for the first time, using in vivo assays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus

Effect of Carqueja hydroalcoholic extract (CHE) in protein homeostasis and Nile red staining. (a) Proteasome activity is increased by CHE treatment. Animals were treated or not with 50 mg/mL CHE from L1 to L4. Proteasome chymotrypsin-like activity was monitored by SLLVY-MCA digestion in L4 worm extracts containing equal amounts of total protein. ***P < 0.05 by two-tailed Student's t-test. Transgenic worms hsp-16-2::GFP (b) and hsp-16-2::GFP (c) were treated or not with 50 mg/mL CHE for 48 h beginning at L1 and then transferred or not to 35°C for 1 h. After a 1 h and 30 min recovery period, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. (b) CHE treatment increased hsp-16-2::GFP expression under stress conditions. (c) Expression of hsp-4::GFP increased in the 50 mg/mL CHE-treated group under standard conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest. (d) Nile red accumulation reduction induced by CHE. Wild-type animals treated or not with 50 mg/mL CHE for 48 h beginning at L1 were maintained in plates containing Nile red. Subsequently, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. CHE treatment reduced lipid fat deposit. ***P < 0.0001 by Mann-Whitney test.
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fig6: Effect of Carqueja hydroalcoholic extract (CHE) in protein homeostasis and Nile red staining. (a) Proteasome activity is increased by CHE treatment. Animals were treated or not with 50 mg/mL CHE from L1 to L4. Proteasome chymotrypsin-like activity was monitored by SLLVY-MCA digestion in L4 worm extracts containing equal amounts of total protein. ***P < 0.05 by two-tailed Student's t-test. Transgenic worms hsp-16-2::GFP (b) and hsp-16-2::GFP (c) were treated or not with 50 mg/mL CHE for 48 h beginning at L1 and then transferred or not to 35°C for 1 h. After a 1 h and 30 min recovery period, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. (b) CHE treatment increased hsp-16-2::GFP expression under stress conditions. (c) Expression of hsp-4::GFP increased in the 50 mg/mL CHE-treated group under standard conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest. (d) Nile red accumulation reduction induced by CHE. Wild-type animals treated or not with 50 mg/mL CHE for 48 h beginning at L1 were maintained in plates containing Nile red. Subsequently, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. CHE treatment reduced lipid fat deposit. ***P < 0.0001 by Mann-Whitney test.

Mentions: The disruption of protein homeostasis underlies the pathologies of most neurodegenerative disorders [37]. To evaluate whether CHE treatment alters protein homeostasis, we measured proteasome activity in animals treated with 50 mg/mL CHE. Proteasome chymotrypsin-like activity was monitored by SLLVY-MCA digestion in L4 worm extracts containing equal amounts of total protein. CHE increased proteasome degradation activity by 5-fold relative to the controls (P < 0.05) (Figure 6(a)).


Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans.

Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P - Oxid Med Cell Longev (2015)

Effect of Carqueja hydroalcoholic extract (CHE) in protein homeostasis and Nile red staining. (a) Proteasome activity is increased by CHE treatment. Animals were treated or not with 50 mg/mL CHE from L1 to L4. Proteasome chymotrypsin-like activity was monitored by SLLVY-MCA digestion in L4 worm extracts containing equal amounts of total protein. ***P < 0.05 by two-tailed Student's t-test. Transgenic worms hsp-16-2::GFP (b) and hsp-16-2::GFP (c) were treated or not with 50 mg/mL CHE for 48 h beginning at L1 and then transferred or not to 35°C for 1 h. After a 1 h and 30 min recovery period, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. (b) CHE treatment increased hsp-16-2::GFP expression under stress conditions. (c) Expression of hsp-4::GFP increased in the 50 mg/mL CHE-treated group under standard conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest. (d) Nile red accumulation reduction induced by CHE. Wild-type animals treated or not with 50 mg/mL CHE for 48 h beginning at L1 were maintained in plates containing Nile red. Subsequently, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. CHE treatment reduced lipid fat deposit. ***P < 0.0001 by Mann-Whitney test.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig6: Effect of Carqueja hydroalcoholic extract (CHE) in protein homeostasis and Nile red staining. (a) Proteasome activity is increased by CHE treatment. Animals were treated or not with 50 mg/mL CHE from L1 to L4. Proteasome chymotrypsin-like activity was monitored by SLLVY-MCA digestion in L4 worm extracts containing equal amounts of total protein. ***P < 0.05 by two-tailed Student's t-test. Transgenic worms hsp-16-2::GFP (b) and hsp-16-2::GFP (c) were treated or not with 50 mg/mL CHE for 48 h beginning at L1 and then transferred or not to 35°C for 1 h. After a 1 h and 30 min recovery period, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. (b) CHE treatment increased hsp-16-2::GFP expression under stress conditions. (c) Expression of hsp-4::GFP increased in the 50 mg/mL CHE-treated group under standard conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest. (d) Nile red accumulation reduction induced by CHE. Wild-type animals treated or not with 50 mg/mL CHE for 48 h beginning at L1 were maintained in plates containing Nile red. Subsequently, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. CHE treatment reduced lipid fat deposit. ***P < 0.0001 by Mann-Whitney test.
Mentions: The disruption of protein homeostasis underlies the pathologies of most neurodegenerative disorders [37]. To evaluate whether CHE treatment alters protein homeostasis, we measured proteasome activity in animals treated with 50 mg/mL CHE. Proteasome chymotrypsin-like activity was monitored by SLLVY-MCA digestion in L4 worm extracts containing equal amounts of total protein. CHE increased proteasome degradation activity by 5-fold relative to the controls (P < 0.05) (Figure 6(a)).

Bottom Line: Carqueja (Baccharis trimera) is a native plant found throughout South America.CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes.Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

ABSTRACT
Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activity in vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potential in vivo are limited. In this study, we used Caenorhabditis elegans as a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in a C. elegans model for Alzheimer's disease. Here, we show for the first time, using in vivo assays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus