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Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans.

Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P - Oxid Med Cell Longev (2015)

Bottom Line: Carqueja (Baccharis trimera) is a native plant found throughout South America.CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes.Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

ABSTRACT
Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activity in vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potential in vivo are limited. In this study, we used Caenorhabditis elegans as a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in a C. elegans model for Alzheimer's disease. Here, we show for the first time, using in vivo assays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus

Effect of Carqueja hydroalcoholic extract (CHE) on redox status in wild-type C. elegans. (a) ROS production. C. elegans was treated or not with 50 mg/mL CHE for 48 h and then submitted to the presence or absence of 5 mM H2O2 for 1 h. CHE reduced ROS production under both standard and stress conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest. Analysis of sod-3::GFP (b), gcs-1::GFP (c), and gst-4::GFP (d). Transgenic worms were treated or not with 50 mg/mL CHE for 48 h beginning at L1 and then with or without the oxidative stress condition. After a 1 h recovery period, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. (b) Expression of sod-3::GFP is reduced in the 50 mg/mL CHE-treated group under standard conditions (c). CHE treatment reduced gcs-1::GFP expression under stress conditions. (d) Expression of gst-4::GFP increased in the 50 mg/mL CHE-treated group under standard conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest.
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fig4: Effect of Carqueja hydroalcoholic extract (CHE) on redox status in wild-type C. elegans. (a) ROS production. C. elegans was treated or not with 50 mg/mL CHE for 48 h and then submitted to the presence or absence of 5 mM H2O2 for 1 h. CHE reduced ROS production under both standard and stress conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest. Analysis of sod-3::GFP (b), gcs-1::GFP (c), and gst-4::GFP (d). Transgenic worms were treated or not with 50 mg/mL CHE for 48 h beginning at L1 and then with or without the oxidative stress condition. After a 1 h recovery period, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. (b) Expression of sod-3::GFP is reduced in the 50 mg/mL CHE-treated group under standard conditions (c). CHE treatment reduced gcs-1::GFP expression under stress conditions. (d) Expression of gst-4::GFP increased in the 50 mg/mL CHE-treated group under standard conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest.

Mentions: Antioxidant phytochemicals can function either directly by scavenging ROS or indirectly through modulation of signaling pathways. To investigate whether the protective effect of 50 mg/mL CHE in treated C. elegans acts directly or indirectly, we measured ROS production and gene expression of sod-3, gcs-1, and gst-4 antioxidant enzymes. CHE treatment reduced ROS production in animals under both standard and stress conditions (Figure 4(a)). CHE treatment reduced sod-3::GFP expression, if compared to untreated animals under standard conditions, but no significant differences were observed between treated and untreated groups under stress conditions (Figure 4(b)). Under stress conditions, CHE treatment reduced gcs-1::GFP expression, if compared to untreated animals, but no significant differences were observed between treated and untreated groups under standard conditions (Figure 4(c)). Expression of gst-4::GFP increased in the CHE-treated group, if compared to that observed in untreated animals under standard conditions. In animals under stress condition, no significant differences were observed between treated and untreated groups (Figure 4(d)). These results suggest that CHE treatment reduces ROS production and inhibits sod-3::GFP under standard conditions and gcs-1::GFP under stress conditions while it activates gst-4::GFP expression under standard conditions.


Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans.

Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P - Oxid Med Cell Longev (2015)

Effect of Carqueja hydroalcoholic extract (CHE) on redox status in wild-type C. elegans. (a) ROS production. C. elegans was treated or not with 50 mg/mL CHE for 48 h and then submitted to the presence or absence of 5 mM H2O2 for 1 h. CHE reduced ROS production under both standard and stress conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest. Analysis of sod-3::GFP (b), gcs-1::GFP (c), and gst-4::GFP (d). Transgenic worms were treated or not with 50 mg/mL CHE for 48 h beginning at L1 and then with or without the oxidative stress condition. After a 1 h recovery period, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. (b) Expression of sod-3::GFP is reduced in the 50 mg/mL CHE-treated group under standard conditions (c). CHE treatment reduced gcs-1::GFP expression under stress conditions. (d) Expression of gst-4::GFP increased in the 50 mg/mL CHE-treated group under standard conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig4: Effect of Carqueja hydroalcoholic extract (CHE) on redox status in wild-type C. elegans. (a) ROS production. C. elegans was treated or not with 50 mg/mL CHE for 48 h and then submitted to the presence or absence of 5 mM H2O2 for 1 h. CHE reduced ROS production under both standard and stress conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest. Analysis of sod-3::GFP (b), gcs-1::GFP (c), and gst-4::GFP (d). Transgenic worms were treated or not with 50 mg/mL CHE for 48 h beginning at L1 and then with or without the oxidative stress condition. After a 1 h recovery period, photographs were taken on a fluorescence microscope and GFP fluorescence signals were measured using NIH Image J software. (b) Expression of sod-3::GFP is reduced in the 50 mg/mL CHE-treated group under standard conditions (c). CHE treatment reduced gcs-1::GFP expression under stress conditions. (d) Expression of gst-4::GFP increased in the 50 mg/mL CHE-treated group under standard conditions. ***P < 0.0001 by Kruskal-Wallis test followed by Dunn's posttest.
Mentions: Antioxidant phytochemicals can function either directly by scavenging ROS or indirectly through modulation of signaling pathways. To investigate whether the protective effect of 50 mg/mL CHE in treated C. elegans acts directly or indirectly, we measured ROS production and gene expression of sod-3, gcs-1, and gst-4 antioxidant enzymes. CHE treatment reduced ROS production in animals under both standard and stress conditions (Figure 4(a)). CHE treatment reduced sod-3::GFP expression, if compared to untreated animals under standard conditions, but no significant differences were observed between treated and untreated groups under stress conditions (Figure 4(b)). Under stress conditions, CHE treatment reduced gcs-1::GFP expression, if compared to untreated animals, but no significant differences were observed between treated and untreated groups under standard conditions (Figure 4(c)). Expression of gst-4::GFP increased in the CHE-treated group, if compared to that observed in untreated animals under standard conditions. In animals under stress condition, no significant differences were observed between treated and untreated groups (Figure 4(d)). These results suggest that CHE treatment reduces ROS production and inhibits sod-3::GFP under standard conditions and gcs-1::GFP under stress conditions while it activates gst-4::GFP expression under standard conditions.

Bottom Line: Carqueja (Baccharis trimera) is a native plant found throughout South America.CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes.Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

ABSTRACT
Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activity in vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potential in vivo are limited. In this study, we used Caenorhabditis elegans as a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in a C. elegans model for Alzheimer's disease. Here, we show for the first time, using in vivo assays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus