Limits...
Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans.

Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P - Oxid Med Cell Longev (2015)

Bottom Line: Carqueja (Baccharis trimera) is a native plant found throughout South America.CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes.Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

ABSTRACT
Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activity in vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potential in vivo are limited. In this study, we used Caenorhabditis elegans as a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in a C. elegans model for Alzheimer's disease. Here, we show for the first time, using in vivo assays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus

Effect of Carqueja hydroalcoholic extract (CHE) on E. coli growth and oxidative stress resistance of C. elegans fed with kanamycin-treated E. coli. (a) E. coli growth. E. coli OP50 growth was evaluated over 5 h in the presence of three different CHE concentrations (0.5, 5, and 50 mg/mL). The OD of the control group at time zero was used to normalize all other OD readings. *Treatment of 0.5 mg/mL CHE decreased bacteria growth after 200 min. **Treatment of 5 mg/mL CHE decreased bacteria growth after 120 min. ***Treatment of 50 mg/mL CHE decreased bacteria growth after 80 min. P < 0.05, determined by a two-tailed Student's t-test. (b) Stress resistance assay on bacteria killed with KAN. Wild-type animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) mixed with either E. coli OP50 or E. coli OP50 treated with 10 mM KAN from L1 to L4 and then submitted to 7.5 mM t-BOOH in M9. The survival was measured at 6, 9, and 12 h. The survival curves show that 50 mg/mL CHE treatment increased C. elegans oxidative stress resistance independent of its antibacterial effect. ***P < 0.001 related to the respective controls by the log-rank (Mantel-Cox) test.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4508469&req=5

fig2: Effect of Carqueja hydroalcoholic extract (CHE) on E. coli growth and oxidative stress resistance of C. elegans fed with kanamycin-treated E. coli. (a) E. coli growth. E. coli OP50 growth was evaluated over 5 h in the presence of three different CHE concentrations (0.5, 5, and 50 mg/mL). The OD of the control group at time zero was used to normalize all other OD readings. *Treatment of 0.5 mg/mL CHE decreased bacteria growth after 200 min. **Treatment of 5 mg/mL CHE decreased bacteria growth after 120 min. ***Treatment of 50 mg/mL CHE decreased bacteria growth after 80 min. P < 0.05, determined by a two-tailed Student's t-test. (b) Stress resistance assay on bacteria killed with KAN. Wild-type animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) mixed with either E. coli OP50 or E. coli OP50 treated with 10 mM KAN from L1 to L4 and then submitted to 7.5 mM t-BOOH in M9. The survival was measured at 6, 9, and 12 h. The survival curves show that 50 mg/mL CHE treatment increased C. elegans oxidative stress resistance independent of its antibacterial effect. ***P < 0.001 related to the respective controls by the log-rank (Mantel-Cox) test.

Mentions: It is known that E. coli has a pathogenic effect on C. elegans, which affects its lifespan and stress resistance [24]. Therefore, we investigated whether the CHE protective effect we observed (Figure 1(b)) could result from inhibition of bacterial growth. When we assayed E. coli OP50 growth over 5 h in the presence of CHE, we observed an antimicrobial effect. This effect was observed after 80 min of incubation with 50 mg/mL CHE and after 120 and 200 min with 5 and 0.5 mg/mL CHE, respectively (Figure 2(a)).


Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans.

Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P - Oxid Med Cell Longev (2015)

Effect of Carqueja hydroalcoholic extract (CHE) on E. coli growth and oxidative stress resistance of C. elegans fed with kanamycin-treated E. coli. (a) E. coli growth. E. coli OP50 growth was evaluated over 5 h in the presence of three different CHE concentrations (0.5, 5, and 50 mg/mL). The OD of the control group at time zero was used to normalize all other OD readings. *Treatment of 0.5 mg/mL CHE decreased bacteria growth after 200 min. **Treatment of 5 mg/mL CHE decreased bacteria growth after 120 min. ***Treatment of 50 mg/mL CHE decreased bacteria growth after 80 min. P < 0.05, determined by a two-tailed Student's t-test. (b) Stress resistance assay on bacteria killed with KAN. Wild-type animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) mixed with either E. coli OP50 or E. coli OP50 treated with 10 mM KAN from L1 to L4 and then submitted to 7.5 mM t-BOOH in M9. The survival was measured at 6, 9, and 12 h. The survival curves show that 50 mg/mL CHE treatment increased C. elegans oxidative stress resistance independent of its antibacterial effect. ***P < 0.001 related to the respective controls by the log-rank (Mantel-Cox) test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508469&req=5

fig2: Effect of Carqueja hydroalcoholic extract (CHE) on E. coli growth and oxidative stress resistance of C. elegans fed with kanamycin-treated E. coli. (a) E. coli growth. E. coli OP50 growth was evaluated over 5 h in the presence of three different CHE concentrations (0.5, 5, and 50 mg/mL). The OD of the control group at time zero was used to normalize all other OD readings. *Treatment of 0.5 mg/mL CHE decreased bacteria growth after 200 min. **Treatment of 5 mg/mL CHE decreased bacteria growth after 120 min. ***Treatment of 50 mg/mL CHE decreased bacteria growth after 80 min. P < 0.05, determined by a two-tailed Student's t-test. (b) Stress resistance assay on bacteria killed with KAN. Wild-type animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) mixed with either E. coli OP50 or E. coli OP50 treated with 10 mM KAN from L1 to L4 and then submitted to 7.5 mM t-BOOH in M9. The survival was measured at 6, 9, and 12 h. The survival curves show that 50 mg/mL CHE treatment increased C. elegans oxidative stress resistance independent of its antibacterial effect. ***P < 0.001 related to the respective controls by the log-rank (Mantel-Cox) test.
Mentions: It is known that E. coli has a pathogenic effect on C. elegans, which affects its lifespan and stress resistance [24]. Therefore, we investigated whether the CHE protective effect we observed (Figure 1(b)) could result from inhibition of bacterial growth. When we assayed E. coli OP50 growth over 5 h in the presence of CHE, we observed an antimicrobial effect. This effect was observed after 80 min of incubation with 50 mg/mL CHE and after 120 and 200 min with 5 and 0.5 mg/mL CHE, respectively (Figure 2(a)).

Bottom Line: Carqueja (Baccharis trimera) is a native plant found throughout South America.CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes.Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

ABSTRACT
Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activity in vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potential in vivo are limited. In this study, we used Caenorhabditis elegans as a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in a C. elegans model for Alzheimer's disease. Here, we show for the first time, using in vivo assays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus