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Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans.

Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P - Oxid Med Cell Longev (2015)

Bottom Line: Carqueja (Baccharis trimera) is a native plant found throughout South America.CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes.Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

ABSTRACT
Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activity in vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potential in vivo are limited. In this study, we used Caenorhabditis elegans as a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in a C. elegans model for Alzheimer's disease. Here, we show for the first time, using in vivo assays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus

Effect of Carqueja hydroalcoholic extract (CHE) on C. elegans survival under standard laboratory and stress conditions. (a) Lifespan. fem-1(hc17) mutants animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) at 25°C beginning at L1. Nematodes were checked daily until all nematodes had died. Log-rank (Mantel-Cox) analysis showed no significant difference between the curves. (b) Stress resistance. Wild-type animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) from L1 to L4 and then submitted to 7.5 mM t-BOOH in M9. Survival was measured at 6, 9, and 12 h. The survival curves show that only 50 mg/mL CHE treatment increased C. elegans oxidative stress resistance, while 0.05 mg/mL CHE decreased oxidative stress resistance. ***P < 0.001 and **P = 0.009 by the log-rank (Mantel-Cox) test.
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fig1: Effect of Carqueja hydroalcoholic extract (CHE) on C. elegans survival under standard laboratory and stress conditions. (a) Lifespan. fem-1(hc17) mutants animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) at 25°C beginning at L1. Nematodes were checked daily until all nematodes had died. Log-rank (Mantel-Cox) analysis showed no significant difference between the curves. (b) Stress resistance. Wild-type animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) from L1 to L4 and then submitted to 7.5 mM t-BOOH in M9. Survival was measured at 6, 9, and 12 h. The survival curves show that only 50 mg/mL CHE treatment increased C. elegans oxidative stress resistance, while 0.05 mg/mL CHE decreased oxidative stress resistance. ***P < 0.001 and **P = 0.009 by the log-rank (Mantel-Cox) test.

Mentions: We first tested whether CHE would affect the lifespan of C. elegans under standard laboratory conditions. Lifespan assays were performed in animals treated with three different CHE concentrations (0.5, 5, and 50 mg/mL) at 25°C. The mean lifespan of all treated animals did not differ significantly from controls lifespan (P = 0.42 for 0.5 mg/mL, 0.82 for 5 mg/mL, and 0.69 for 50 mg/mL) (Table 2). These findings indicate that, under standard conditions, CHE treatments at these concentrations did not alter C. elegans lifespan (Figure 1(a)).


Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans.

Paiva FA, Bonomo Lde F, Boasquivis PF, de Paula IT, Guerra JF, Leal WM, Silva ME, Pedrosa ML, Oliveira Rde P - Oxid Med Cell Longev (2015)

Effect of Carqueja hydroalcoholic extract (CHE) on C. elegans survival under standard laboratory and stress conditions. (a) Lifespan. fem-1(hc17) mutants animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) at 25°C beginning at L1. Nematodes were checked daily until all nematodes had died. Log-rank (Mantel-Cox) analysis showed no significant difference between the curves. (b) Stress resistance. Wild-type animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) from L1 to L4 and then submitted to 7.5 mM t-BOOH in M9. Survival was measured at 6, 9, and 12 h. The survival curves show that only 50 mg/mL CHE treatment increased C. elegans oxidative stress resistance, while 0.05 mg/mL CHE decreased oxidative stress resistance. ***P < 0.001 and **P = 0.009 by the log-rank (Mantel-Cox) test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508469&req=5

fig1: Effect of Carqueja hydroalcoholic extract (CHE) on C. elegans survival under standard laboratory and stress conditions. (a) Lifespan. fem-1(hc17) mutants animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) at 25°C beginning at L1. Nematodes were checked daily until all nematodes had died. Log-rank (Mantel-Cox) analysis showed no significant difference between the curves. (b) Stress resistance. Wild-type animals were treated or not with three different CHE concentrations (0.5, 5, and 50 mg/mL) from L1 to L4 and then submitted to 7.5 mM t-BOOH in M9. Survival was measured at 6, 9, and 12 h. The survival curves show that only 50 mg/mL CHE treatment increased C. elegans oxidative stress resistance, while 0.05 mg/mL CHE decreased oxidative stress resistance. ***P < 0.001 and **P = 0.009 by the log-rank (Mantel-Cox) test.
Mentions: We first tested whether CHE would affect the lifespan of C. elegans under standard laboratory conditions. Lifespan assays were performed in animals treated with three different CHE concentrations (0.5, 5, and 50 mg/mL) at 25°C. The mean lifespan of all treated animals did not differ significantly from controls lifespan (P = 0.42 for 0.5 mg/mL, 0.82 for 5 mg/mL, and 0.69 for 50 mg/mL) (Table 2). These findings indicate that, under standard conditions, CHE treatments at these concentrations did not alter C. elegans lifespan (Figure 1(a)).

Bottom Line: Carqueja (Baccharis trimera) is a native plant found throughout South America.CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes.Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

ABSTRACT
Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activity in vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potential in vivo are limited. In this study, we used Caenorhabditis elegans as a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in a C. elegans model for Alzheimer's disease. Here, we show for the first time, using in vivo assays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus