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Hepatitis C Virus Core Mutations Associated with False-Negative Serological Results for Genotype 3a Core Antigen.

Nguyen LT, Dunford L, Freitas I, Holder P, Nguyen LA, O'Gorman J, Connell J, Carr M, Hall W, De Gascun C - J. Clin. Microbiol. (2015)

Bottom Line: Genetic characterization of the genotype 3a (GT3a) hepatitis C virus (HCV) core region from HCV core antigen (HCVcAg)-negative/RNA-positive cases and HCVcAg-positive/RNA-positive controls identified significant associations between the substitutions A48T and T49A/P and failure to detect HCVcAg (P < 0.05).Polymorphisms at residues 48 and 49 in the core protein are present across all major epidemic and endemic GTs.These findings have implications for HCV diagnosis, particularly in low-income regions in which GT3a HCV is endemic.

View Article: PubMed Central - PubMed

Affiliation: Ireland Vietnam Blood-Borne Virus Initiative, Dublin, Ireland, and Hanoi, Vietnam Centre for Research in Infectious Diseases, School of Medicine and Medical Science, University College Dublin, Belfield, Ireland Laboratory for Molecular Diagnostics, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam crid.office@ucd.ie.

No MeSH data available.


Related in: MedlinePlus

Scatter plots depicting the correlation between HCVcAg (log10 fmol/liter) and HCV RNA (log10 IU/ml) measurements. Arrows, samples in which A48T (n = 2) and T49A (n = 3) were identified in the GT3a HCV core protein.
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Figure 1: Scatter plots depicting the correlation between HCVcAg (log10 fmol/liter) and HCV RNA (log10 IU/ml) measurements. Arrows, samples in which A48T (n = 2) and T49A (n = 3) were identified in the GT3a HCV core protein.

Mentions: HCVcAg- and RNA-positive samples for GT1a (n = 261), GT1b (n = 79), and GT3 (n = 171) were evaluated. Strong positive correlations between HCVcAg and RNA levels (P < 0.0001) were observed for all three GTs (Fig. 1). However, the correlation coefficient for the correlation of HCVcAg and RNA levels was lower for GT3 (r = 0.79) than for GT1a (r = 0.87) and GT1b (r = 0.86). Greater variance for GT3, compared to GT1a and GT1b, was apparent (Fig. 1). Furthermore, the HCVcAg/RNA ratio for GT3 was significantly lower than those for GT1a and GT1b (P < 0.0001), while there was no significant difference in the HCVcAg/RNA ratios for GT1a and GT1b (P = 0.44), indicating the underquantification of HCVcAg, relative to the corresponding viral loads, for GT3 samples.


Hepatitis C Virus Core Mutations Associated with False-Negative Serological Results for Genotype 3a Core Antigen.

Nguyen LT, Dunford L, Freitas I, Holder P, Nguyen LA, O'Gorman J, Connell J, Carr M, Hall W, De Gascun C - J. Clin. Microbiol. (2015)

Scatter plots depicting the correlation between HCVcAg (log10 fmol/liter) and HCV RNA (log10 IU/ml) measurements. Arrows, samples in which A48T (n = 2) and T49A (n = 3) were identified in the GT3a HCV core protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508445&req=5

Figure 1: Scatter plots depicting the correlation between HCVcAg (log10 fmol/liter) and HCV RNA (log10 IU/ml) measurements. Arrows, samples in which A48T (n = 2) and T49A (n = 3) were identified in the GT3a HCV core protein.
Mentions: HCVcAg- and RNA-positive samples for GT1a (n = 261), GT1b (n = 79), and GT3 (n = 171) were evaluated. Strong positive correlations between HCVcAg and RNA levels (P < 0.0001) were observed for all three GTs (Fig. 1). However, the correlation coefficient for the correlation of HCVcAg and RNA levels was lower for GT3 (r = 0.79) than for GT1a (r = 0.87) and GT1b (r = 0.86). Greater variance for GT3, compared to GT1a and GT1b, was apparent (Fig. 1). Furthermore, the HCVcAg/RNA ratio for GT3 was significantly lower than those for GT1a and GT1b (P < 0.0001), while there was no significant difference in the HCVcAg/RNA ratios for GT1a and GT1b (P = 0.44), indicating the underquantification of HCVcAg, relative to the corresponding viral loads, for GT3 samples.

Bottom Line: Genetic characterization of the genotype 3a (GT3a) hepatitis C virus (HCV) core region from HCV core antigen (HCVcAg)-negative/RNA-positive cases and HCVcAg-positive/RNA-positive controls identified significant associations between the substitutions A48T and T49A/P and failure to detect HCVcAg (P < 0.05).Polymorphisms at residues 48 and 49 in the core protein are present across all major epidemic and endemic GTs.These findings have implications for HCV diagnosis, particularly in low-income regions in which GT3a HCV is endemic.

View Article: PubMed Central - PubMed

Affiliation: Ireland Vietnam Blood-Borne Virus Initiative, Dublin, Ireland, and Hanoi, Vietnam Centre for Research in Infectious Diseases, School of Medicine and Medical Science, University College Dublin, Belfield, Ireland Laboratory for Molecular Diagnostics, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam crid.office@ucd.ie.

No MeSH data available.


Related in: MedlinePlus