Limits...
Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

No MeSH data available.


Related in: MedlinePlus

DPPH radical scavenging activity (a) and ABTS•+ scavenging assay (b).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4508389&req=5

fig5: DPPH radical scavenging activity (a) and ABTS•+ scavenging assay (b).

Mentions: DPPH radical scavenging activity test aims to measure the capacity of sample to scavenge the stable radical DPPH formed in solution by donation of hydrogen atom or an electron [33]. As shown in (Figure 5(a)), the IC50 value of liriodendrin is 13.61 μg/mL, which is less important than that of vitamin C (IC50 = 6.98 μg/mL).


Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

DPPH radical scavenging activity (a) and ABTS•+ scavenging assay (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508389&req=5

fig5: DPPH radical scavenging activity (a) and ABTS•+ scavenging assay (b).
Mentions: DPPH radical scavenging activity test aims to measure the capacity of sample to scavenge the stable radical DPPH formed in solution by donation of hydrogen atom or an electron [33]. As shown in (Figure 5(a)), the IC50 value of liriodendrin is 13.61 μg/mL, which is less important than that of vitamin C (IC50 = 6.98 μg/mL).

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

No MeSH data available.


Related in: MedlinePlus