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Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatograms of samples before treatment (a) and after treatment (b) on X-5 resin; liriodendrin purified by crystallization (c).
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fig4: HPLC chromatograms of samples before treatment (a) and after treatment (b) on X-5 resin; liriodendrin purified by crystallization (c).

Mentions: After purification on X-5 resin, the purity of liriodendrin increased from 0.85% of S. cuneata extracts to 41.42%, and the liriodendrin of recovery rate was 88.9%. Such crystallization resulted in a product with 97.48% purity and the recovery rate was 63.7% (Table 4). As shown in Figures 4(a) and 4(b), the HPLC chromatograms of S. cuneata extracts before and after treatment on X-5 resin were compared. Figure 4(c) showed that the purity of liriodendrin crystals was determined to be 97.48% by HPLC. For further chemical structure identification of liriodendrin, NMR analysis was performed and the data was shown as follows: 1H-NMR (DMSO-d6, 400 MHz) δ: 6.65 (4H, s, H-2, 2′, 6, 6′), 4.93 (2H, d, J = 6.8 Hz, H-1′′), 4.66 (2H, d, J = 3.6 Hz, H-7, 7′), 4.31 (2H, m, Hb-9, 9′), 4.19 (2H, m, Ha-9, 9′), 3.75 (12H, s, 4 × OCH3); 13C-NMR (DMSO-d6, 100 MHz) δc: 152.6 (C-3, 3′, 5, 5′), 137.1 (C-4, 4′), 133.8 (C-1, 1′), 104.3 (C-2, 2′, 6, 6′), 102.7 (C-1′′), 85.0 (C-7, 7′), 77.2 (C-5′′), 76.5 (C-3′′), 74.1 (C-2′′), 71.3 (C-9, 9′), 70.0 (C-4′′), 60.9 (C-6′′), 56.4 (-OCH3), 53.6 (C-8, 8′). The data were identical with those reported in the literature [27].


Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

HPLC chromatograms of samples before treatment (a) and after treatment (b) on X-5 resin; liriodendrin purified by crystallization (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508389&req=5

fig4: HPLC chromatograms of samples before treatment (a) and after treatment (b) on X-5 resin; liriodendrin purified by crystallization (c).
Mentions: After purification on X-5 resin, the purity of liriodendrin increased from 0.85% of S. cuneata extracts to 41.42%, and the liriodendrin of recovery rate was 88.9%. Such crystallization resulted in a product with 97.48% purity and the recovery rate was 63.7% (Table 4). As shown in Figures 4(a) and 4(b), the HPLC chromatograms of S. cuneata extracts before and after treatment on X-5 resin were compared. Figure 4(c) showed that the purity of liriodendrin crystals was determined to be 97.48% by HPLC. For further chemical structure identification of liriodendrin, NMR analysis was performed and the data was shown as follows: 1H-NMR (DMSO-d6, 400 MHz) δ: 6.65 (4H, s, H-2, 2′, 6, 6′), 4.93 (2H, d, J = 6.8 Hz, H-1′′), 4.66 (2H, d, J = 3.6 Hz, H-7, 7′), 4.31 (2H, m, Hb-9, 9′), 4.19 (2H, m, Ha-9, 9′), 3.75 (12H, s, 4 × OCH3); 13C-NMR (DMSO-d6, 100 MHz) δc: 152.6 (C-3, 3′, 5, 5′), 137.1 (C-4, 4′), 133.8 (C-1, 1′), 104.3 (C-2, 2′, 6, 6′), 102.7 (C-1′′), 85.0 (C-7, 7′), 77.2 (C-5′′), 76.5 (C-3′′), 74.1 (C-2′′), 71.3 (C-9, 9′), 70.0 (C-4′′), 60.9 (C-6′′), 56.4 (-OCH3), 53.6 (C-8, 8′). The data were identical with those reported in the literature [27].

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

No MeSH data available.


Related in: MedlinePlus