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Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

No MeSH data available.


Related in: MedlinePlus

Dynamic adsorption and desorption test curves on X-5 resin. (a) Dynamic leakage curve, (b) gradient elution curve, and (c) isocratic desorption curve of liriodendrin on X-5 resin column at 25°C.
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fig3: Dynamic adsorption and desorption test curves on X-5 resin. (a) Dynamic leakage curve, (b) gradient elution curve, and (c) isocratic desorption curve of liriodendrin on X-5 resin column at 25°C.

Mentions: Dynamic breakthrough tests were studies based on the feed concentration, the volume of effluent, flow rate, and temperature. The initial feed concentration of liriodendrin was 1.705 mg/mL (from Section 3.3) and the temperature at 25°C, and then the effect of flow rate was investigated. As shown in Figure 3(a), the different flow rates showed noticeable different breakthrough volume. When the flow rate was 1 BV/h, the best adsorption performance could be obtained. The breakthrough volume of liriodendrin on X-5 resin was 55 mL (about 2 BV) at a flow rate of 1 BV/h, when the liriodendrin concentration in effluents reached 1% of the initial concentration [18]. The adsorption capacity of liriodendrin on X-5 resin was 15.630 mg/g dry resin.


Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

Dynamic adsorption and desorption test curves on X-5 resin. (a) Dynamic leakage curve, (b) gradient elution curve, and (c) isocratic desorption curve of liriodendrin on X-5 resin column at 25°C.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508389&req=5

fig3: Dynamic adsorption and desorption test curves on X-5 resin. (a) Dynamic leakage curve, (b) gradient elution curve, and (c) isocratic desorption curve of liriodendrin on X-5 resin column at 25°C.
Mentions: Dynamic breakthrough tests were studies based on the feed concentration, the volume of effluent, flow rate, and temperature. The initial feed concentration of liriodendrin was 1.705 mg/mL (from Section 3.3) and the temperature at 25°C, and then the effect of flow rate was investigated. As shown in Figure 3(a), the different flow rates showed noticeable different breakthrough volume. When the flow rate was 1 BV/h, the best adsorption performance could be obtained. The breakthrough volume of liriodendrin on X-5 resin was 55 mL (about 2 BV) at a flow rate of 1 BV/h, when the liriodendrin concentration in effluents reached 1% of the initial concentration [18]. The adsorption capacity of liriodendrin on X-5 resin was 15.630 mg/g dry resin.

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

No MeSH data available.


Related in: MedlinePlus