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Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

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Adsorption kinetics curves (a) and adsorption isotherms curves (b) for liriodendrin on D101 and X-5 resins at 25°C.
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fig2: Adsorption kinetics curves (a) and adsorption isotherms curves (b) for liriodendrin on D101 and X-5 resins at 25°C.

Mentions: Adsorption kinetics on D101 and X-5 resins were evaluated at 25°C. Adsorption kinetic curves were shown in Figure 2(a). For the two resins, the adsorption capacities toward liriodendrin increased rapidly in the 30 min, and an asymptotic curve was reached at about 120 min. The fast initial adsorption was likely due to rapid attachment of liriodendrin to the surface of resin and an asymptotic period due to diffusion of liriodendrin into the micropores of resin with high intraparticle mass transfer resistance, which indicated that the behaviors of two resins belonged to the fast adsorption resin type [31]. The parameters of three kinetic models were shown in Table 3(a). According to the calculated correlation coefficient (R2 ≥ 0.99), the pseudo-second-order kinetic model performed better to describe the adsorption process on X-5 and D101 resins, which suggested that the adsorption rate was controlled by chemical adsorption mechanism through sharing or exchange of electrons between adsorbate and adsorbent [32]. In terms of the rate constant k2, the adsorption rate of X-5 and D101 resin was the same.


Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

Adsorption kinetics curves (a) and adsorption isotherms curves (b) for liriodendrin on D101 and X-5 resins at 25°C.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508389&req=5

fig2: Adsorption kinetics curves (a) and adsorption isotherms curves (b) for liriodendrin on D101 and X-5 resins at 25°C.
Mentions: Adsorption kinetics on D101 and X-5 resins were evaluated at 25°C. Adsorption kinetic curves were shown in Figure 2(a). For the two resins, the adsorption capacities toward liriodendrin increased rapidly in the 30 min, and an asymptotic curve was reached at about 120 min. The fast initial adsorption was likely due to rapid attachment of liriodendrin to the surface of resin and an asymptotic period due to diffusion of liriodendrin into the micropores of resin with high intraparticle mass transfer resistance, which indicated that the behaviors of two resins belonged to the fast adsorption resin type [31]. The parameters of three kinetic models were shown in Table 3(a). According to the calculated correlation coefficient (R2 ≥ 0.99), the pseudo-second-order kinetic model performed better to describe the adsorption process on X-5 and D101 resins, which suggested that the adsorption rate was controlled by chemical adsorption mechanism through sharing or exchange of electrons between adsorbate and adsorbent [32]. In terms of the rate constant k2, the adsorption rate of X-5 and D101 resin was the same.

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

No MeSH data available.


Related in: MedlinePlus