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Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

No MeSH data available.


Related in: MedlinePlus

Chemical structure of liriodendrin.
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fig1: Chemical structure of liriodendrin.

Mentions: Sargentodoxa cuneata (Lardizabalaceae) is a well-known herb of traditional Chinese medicine in China for treating abdominal pain due to acute appendicitis, sore and ulcer, dysmenorrheal, amenorrhea, traumatic swelling, and rheumatic arthritis. Modern studies have demonstrated that it possesses various pharmacological effects such as antiviral [1, 2], antibacterial [3], antioxidant [4, 5], antitumor [6], anti-inflammatory [7], resistance myocardial ischemia [8], and antithrombotic [9]. Among various active ingredients, liriodendrin (Figure 1) has been proved to be one of the bioactive compounds due to possessing of anti-inflammatory, antinociceptive [10], antiarrhythmic [11], antimyocardial ischemia [8], calcium channel antagonistic [12], inhibitory activity on HepG-2 cells [13], resisting glutamate-induced PC12 cell damage [14], increasing of heat shock factor 1 expression [15], and protecting SH-SY5Y cell effects [16].


Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin.

Li DH, Wang Y, Lv YS, Liu JH, Yang L, Zhang SK, Zhuo YZ - Biomed Res Int (2015)

Chemical structure of liriodendrin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508389&req=5

fig1: Chemical structure of liriodendrin.
Mentions: Sargentodoxa cuneata (Lardizabalaceae) is a well-known herb of traditional Chinese medicine in China for treating abdominal pain due to acute appendicitis, sore and ulcer, dysmenorrheal, amenorrhea, traumatic swelling, and rheumatic arthritis. Modern studies have demonstrated that it possesses various pharmacological effects such as antiviral [1, 2], antibacterial [3], antioxidant [4, 5], antitumor [6], anti-inflammatory [7], resistance myocardial ischemia [8], and antithrombotic [9]. Among various active ingredients, liriodendrin (Figure 1) has been proved to be one of the bioactive compounds due to possessing of anti-inflammatory, antinociceptive [10], antiarrhythmic [11], antimyocardial ischemia [8], calcium channel antagonistic [12], inhibitory activity on HepG-2 cells [13], resisting glutamate-induced PC12 cell damage [14], increasing of heat shock factor 1 expression [15], and protecting SH-SY5Y cell effects [16].

Bottom Line: X-5 resin was selected as the most suitable medium for liriodendrin purification.The purified product possessed strong antioxidant activity.In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China.

ABSTRACT
The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.

No MeSH data available.


Related in: MedlinePlus