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The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats.

Mehanna RA, Nabil I, Attia N, Bary AA, Razek KA, Ahmed TA, Elsayed F - Biomed Res Int (2015)

Bottom Line: Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups.Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength.Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

View Article: PubMed Central - PubMed

Affiliation: Medical Physiology Department, Faculty of Medicine, Alexandria University, Dr Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, Egypt ; Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria 21514, Egypt.

ABSTRACT
Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (FG + SCs), and fibrin + CM group (FG + CM). Healing wounds were evaluated functionally and microscopically. Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups. Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength. Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

No MeSH data available.


Related in: MedlinePlus

Model of progression of wound healing over time and wound measurement. (a)–(f) The shown graduated instrument measured the wound length and width. (a) Day 0. (b) Day 7. (c) Day14. (d) Day 21. (e) Day 28. (f) Day 35 complete healing. (g) Line graph of wound healing progression over time. Vertical error bars represented the standard deviation of the reported mean values (n = 10).
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fig2: Model of progression of wound healing over time and wound measurement. (a)–(f) The shown graduated instrument measured the wound length and width. (a) Day 0. (b) Day 7. (c) Day14. (d) Day 21. (e) Day 28. (f) Day 35 complete healing. (g) Line graph of wound healing progression over time. Vertical error bars represented the standard deviation of the reported mean values (n = 10).

Mentions: Measurement of wounds' size was gradually decreasing over time (Figure 2). Wounds' size in FG + SCs and FG + CM groups showed significant decrease in comparison to the CG and FG during most of healing duration (till day 27) after which they were mostly indifferent till the point of complete healing. On the other hand, there was no difference in wounds' size neither between the CG and FG nor between FG + SCs and FG + CM groups along the whole wound healing duration (supplementary table in Supplementary Material available online at http://dx.doi.org/10.1155/2015/846062). However our data (Table 1) showed no significant difference between groups regarding the duration of wound closure (p = 0.426).


The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats.

Mehanna RA, Nabil I, Attia N, Bary AA, Razek KA, Ahmed TA, Elsayed F - Biomed Res Int (2015)

Model of progression of wound healing over time and wound measurement. (a)–(f) The shown graduated instrument measured the wound length and width. (a) Day 0. (b) Day 7. (c) Day14. (d) Day 21. (e) Day 28. (f) Day 35 complete healing. (g) Line graph of wound healing progression over time. Vertical error bars represented the standard deviation of the reported mean values (n = 10).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508387&req=5

fig2: Model of progression of wound healing over time and wound measurement. (a)–(f) The shown graduated instrument measured the wound length and width. (a) Day 0. (b) Day 7. (c) Day14. (d) Day 21. (e) Day 28. (f) Day 35 complete healing. (g) Line graph of wound healing progression over time. Vertical error bars represented the standard deviation of the reported mean values (n = 10).
Mentions: Measurement of wounds' size was gradually decreasing over time (Figure 2). Wounds' size in FG + SCs and FG + CM groups showed significant decrease in comparison to the CG and FG during most of healing duration (till day 27) after which they were mostly indifferent till the point of complete healing. On the other hand, there was no difference in wounds' size neither between the CG and FG nor between FG + SCs and FG + CM groups along the whole wound healing duration (supplementary table in Supplementary Material available online at http://dx.doi.org/10.1155/2015/846062). However our data (Table 1) showed no significant difference between groups regarding the duration of wound closure (p = 0.426).

Bottom Line: Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups.Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength.Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

View Article: PubMed Central - PubMed

Affiliation: Medical Physiology Department, Faculty of Medicine, Alexandria University, Dr Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, Egypt ; Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria 21514, Egypt.

ABSTRACT
Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (FG + SCs), and fibrin + CM group (FG + CM). Healing wounds were evaluated functionally and microscopically. Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups. Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength. Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

No MeSH data available.


Related in: MedlinePlus