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The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats.

Mehanna RA, Nabil I, Attia N, Bary AA, Razek KA, Ahmed TA, Elsayed F - Biomed Res Int (2015)

Bottom Line: Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups.Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength.Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

View Article: PubMed Central - PubMed

Affiliation: Medical Physiology Department, Faculty of Medicine, Alexandria University, Dr Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, Egypt ; Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria 21514, Egypt.

ABSTRACT
Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (FG + SCs), and fibrin + CM group (FG + CM). Healing wounds were evaluated functionally and microscopically. Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups. Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength. Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

No MeSH data available.


Related in: MedlinePlus

Morphological and phenotypic characteristics of BM-MSCs. The cells isolated from rat bone marrow exhibited spindle shaped fibroblast-like morphology. (a) Primary culture, passage zero (P0), (b) P1, (c) P2, and (d) P3. (Phase contrast microscope, scale bar = 100 µm.) (e) A representative flow cytometric analysis of cell-surface markers of BM-MSCs at P3. Cells were labeled with antibodies against MSC marker (CD44-PE) and hematopoietic antigen (CD45-FITC); M1 is the % of cells positive for both markers.
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fig1: Morphological and phenotypic characteristics of BM-MSCs. The cells isolated from rat bone marrow exhibited spindle shaped fibroblast-like morphology. (a) Primary culture, passage zero (P0), (b) P1, (c) P2, and (d) P3. (Phase contrast microscope, scale bar = 100 µm.) (e) A representative flow cytometric analysis of cell-surface markers of BM-MSCs at P3. Cells were labeled with antibodies against MSC marker (CD44-PE) and hematopoietic antigen (CD45-FITC); M1 is the % of cells positive for both markers.

Mentions: Shortly after culture, fusiform or polygonal cells showed multiple small cytoplasmic projections adhering to the tissue culture plastic. Cells continue to proliferate and became confluent within two weeks, in general. We first focused on the morphology of the cultured cells at different passages. This is shown in Figures 1(a)–1(d) where cells depicted heterogeneous morphology, exhibiting large, flattened, and slim spindle shapes. Cultures of P0–P3 were photographed when they were almost semiconfluent, denoting that cellular morphology and proliferative potential were maintained in the passages adopted in this study.


The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats.

Mehanna RA, Nabil I, Attia N, Bary AA, Razek KA, Ahmed TA, Elsayed F - Biomed Res Int (2015)

Morphological and phenotypic characteristics of BM-MSCs. The cells isolated from rat bone marrow exhibited spindle shaped fibroblast-like morphology. (a) Primary culture, passage zero (P0), (b) P1, (c) P2, and (d) P3. (Phase contrast microscope, scale bar = 100 µm.) (e) A representative flow cytometric analysis of cell-surface markers of BM-MSCs at P3. Cells were labeled with antibodies against MSC marker (CD44-PE) and hematopoietic antigen (CD45-FITC); M1 is the % of cells positive for both markers.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508387&req=5

fig1: Morphological and phenotypic characteristics of BM-MSCs. The cells isolated from rat bone marrow exhibited spindle shaped fibroblast-like morphology. (a) Primary culture, passage zero (P0), (b) P1, (c) P2, and (d) P3. (Phase contrast microscope, scale bar = 100 µm.) (e) A representative flow cytometric analysis of cell-surface markers of BM-MSCs at P3. Cells were labeled with antibodies against MSC marker (CD44-PE) and hematopoietic antigen (CD45-FITC); M1 is the % of cells positive for both markers.
Mentions: Shortly after culture, fusiform or polygonal cells showed multiple small cytoplasmic projections adhering to the tissue culture plastic. Cells continue to proliferate and became confluent within two weeks, in general. We first focused on the morphology of the cultured cells at different passages. This is shown in Figures 1(a)–1(d) where cells depicted heterogeneous morphology, exhibiting large, flattened, and slim spindle shapes. Cultures of P0–P3 were photographed when they were almost semiconfluent, denoting that cellular morphology and proliferative potential were maintained in the passages adopted in this study.

Bottom Line: Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups.Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength.Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

View Article: PubMed Central - PubMed

Affiliation: Medical Physiology Department, Faculty of Medicine, Alexandria University, Dr Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, Egypt ; Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria 21514, Egypt.

ABSTRACT
Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (FG + SCs), and fibrin + CM group (FG + CM). Healing wounds were evaluated functionally and microscopically. Eight days after injury, number of CD68+ macrophages infiltrating granulation tissue was considerably higher in the latter two groups. Although--later--none of the groups depicted a substantially different healing rate, the quality of regenerated skin was significantly boosted by the application of either BM-MSCs or their CM both (1) structurally as demonstrated by the obviously increased mean area percent of collagen fibers in Masson's trichrome-stained skin biopsies and (2) functionally as supported by the interestingly improved epidermal barrier as well as dermal tensile strength. Thus, we conclude that topically applied BM-MSCs and their CM-via fibrin vehicle--could effectively improve the quality of healed skin in chronic excisional wounds in rats, albeit without true acceleration of wound closure.

No MeSH data available.


Related in: MedlinePlus