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Biological monitoring and the influence of genetic polymorphism of As3MT and GSTs on distribution of urinary arsenic species in occupational exposure workers.

Janasik B, Reszka E, Stanislawska M, Wieczorek E, Fendler W, Wasowicz W - Int Arch Occup Environ Health (2014)

Bottom Line: A significant correlation (p < 0.05) was observed between arsenic in air and sum of iAs +MMA and iAs.As3MT (rs3740400) GG homozygotes showed significantly (p < 0.05) higher %iAs (21.8 ± 2.0) in urine than GC+CC heterozygotes (16.0 ± 2.1).Nevertheless, further studies investigating genetic polymorphism in occupational conditions are required.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland, beatajan@imp.lodz.pl.

ABSTRACT

Purpose: To examine the differences in urinary arsenic metabolism patterns in men affected by occupational exposure, we performed a study on 149 participants—workers of a copper mill and 52 healthy controls without occupational exposure. To elucidate the role of genetic factors in arsenic (As) metabolism, we studied the associations of six polymorphisms: As3MT Met287Thr (T>C) in exon 9; As3MT A>G in 5'UTR; As3MT C>G in intron 6; As3MT T>G in intron 1; GSTP1 Ile105Val and GSTO2 T>C.

Methods: Air samples were collected using individual samplers during work shift. Urine samples were analyzed for total arsenic and arsenic chemical forms (As(III); As(V), MMA, DMA, AsB) using HPLC-ICP-MS. A specific polymerase chain reaction was done for the amplification of exons and flanking regions of As3MT and GSTs.

Results: The geometric mean arsenic concentrations in the air were 27.6 ± 4.9 µg/m(3). A significant correlation (p < 0.05) was observed between arsenic in air and sum of iAs +MMA and iAs. As3MT (rs3740400) GG homozygotes showed significantly (p < 0.05) higher %iAs (21.8 ± 2.0) in urine than GC+CC heterozygotes (16.0 ± 2.1). A strong association between the gene variants and As species in urine was observed for GSTO2 (rs156697) polymorphism.

Conclusions: The findings of the study point out that the concentration of iAs or the sum of iAs + MMA in urine can be a reliable biological indicator of occupational exposure to arsenic. This study demonstrates that As3MT and/or GSTs genotype may influence As metabolism. Nevertheless, further studies investigating genetic polymorphism in occupational conditions are required.

No MeSH data available.


Related in: MedlinePlus

LD values for As3MT SNPs
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Fig2: LD values for As3MT SNPs

Mentions: Haplotype analysis of the four genotyped loci in As3MT showed that rs11191439 and rs3740393 were in high linkage disequilibrium similarl to rs3740400 and rs7085104. Therefore, only rs3740393 and rs3740400 were chosen for the adjusted multivariate analysis. The effects were the strongest for the rs3740400 genotypes and were significantly associated with higher %iAs. (Table 4; Fig. 2).Table 4


Biological monitoring and the influence of genetic polymorphism of As3MT and GSTs on distribution of urinary arsenic species in occupational exposure workers.

Janasik B, Reszka E, Stanislawska M, Wieczorek E, Fendler W, Wasowicz W - Int Arch Occup Environ Health (2014)

LD values for As3MT SNPs
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508369&req=5

Fig2: LD values for As3MT SNPs
Mentions: Haplotype analysis of the four genotyped loci in As3MT showed that rs11191439 and rs3740393 were in high linkage disequilibrium similarl to rs3740400 and rs7085104. Therefore, only rs3740393 and rs3740400 were chosen for the adjusted multivariate analysis. The effects were the strongest for the rs3740400 genotypes and were significantly associated with higher %iAs. (Table 4; Fig. 2).Table 4

Bottom Line: A significant correlation (p < 0.05) was observed between arsenic in air and sum of iAs +MMA and iAs.As3MT (rs3740400) GG homozygotes showed significantly (p < 0.05) higher %iAs (21.8 ± 2.0) in urine than GC+CC heterozygotes (16.0 ± 2.1).Nevertheless, further studies investigating genetic polymorphism in occupational conditions are required.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland, beatajan@imp.lodz.pl.

ABSTRACT

Purpose: To examine the differences in urinary arsenic metabolism patterns in men affected by occupational exposure, we performed a study on 149 participants—workers of a copper mill and 52 healthy controls without occupational exposure. To elucidate the role of genetic factors in arsenic (As) metabolism, we studied the associations of six polymorphisms: As3MT Met287Thr (T>C) in exon 9; As3MT A>G in 5'UTR; As3MT C>G in intron 6; As3MT T>G in intron 1; GSTP1 Ile105Val and GSTO2 T>C.

Methods: Air samples were collected using individual samplers during work shift. Urine samples were analyzed for total arsenic and arsenic chemical forms (As(III); As(V), MMA, DMA, AsB) using HPLC-ICP-MS. A specific polymerase chain reaction was done for the amplification of exons and flanking regions of As3MT and GSTs.

Results: The geometric mean arsenic concentrations in the air were 27.6 ± 4.9 µg/m(3). A significant correlation (p < 0.05) was observed between arsenic in air and sum of iAs +MMA and iAs. As3MT (rs3740400) GG homozygotes showed significantly (p < 0.05) higher %iAs (21.8 ± 2.0) in urine than GC+CC heterozygotes (16.0 ± 2.1). A strong association between the gene variants and As species in urine was observed for GSTO2 (rs156697) polymorphism.

Conclusions: The findings of the study point out that the concentration of iAs or the sum of iAs + MMA in urine can be a reliable biological indicator of occupational exposure to arsenic. This study demonstrates that As3MT and/or GSTs genotype may influence As metabolism. Nevertheless, further studies investigating genetic polymorphism in occupational conditions are required.

No MeSH data available.


Related in: MedlinePlus