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Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Orb2A Is Required during Memory Acquisition and Orb2B during Memory Consolidation(Upper panel) Schematic of the rescue experiment with depicted domain organization of the proteins involved. (Lower panel) The plots indicate mean learning indices ± SEM of the temporal rescue of the orb2Orb2BΔQ and orb2mCPEB2RBD mutants with either UAS-orb2A/UAS-orb2AΔQ or UAS-orb2B/UAS-orb2BRBD∗ under control of MB247-Gal4 and tubGal80ts, trained and tested in single-pair assays with mated females for long-term memory and treated according to the regime outlined in the center of the panel. p values are for H0 LI = 0; ∗∗∗p < 0.001; ∗∗p < 0.01. See Table S10.
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fig6: Orb2A Is Required during Memory Acquisition and Orb2B during Memory Consolidation(Upper panel) Schematic of the rescue experiment with depicted domain organization of the proteins involved. (Lower panel) The plots indicate mean learning indices ± SEM of the temporal rescue of the orb2Orb2BΔQ and orb2mCPEB2RBD mutants with either UAS-orb2A/UAS-orb2AΔQ or UAS-orb2B/UAS-orb2BRBD∗ under control of MB247-Gal4 and tubGal80ts, trained and tested in single-pair assays with mated females for long-term memory and treated according to the regime outlined in the center of the panel. p values are for H0 LI = 0; ∗∗∗p < 0.001; ∗∗p < 0.01. See Table S10.

Mentions: To examine temporal requirement of Orb2A and its Q domain, we expressed in the MB neurons either wild-type Orb2A (UAS-orb2AGFP) or Orb2A with the Q domain deleted (UAS-orb2ΔQGFP) under control of MB247-Gal4 and temperature-gated tub-Gal80ts in the flies lacking the A isoform and the Q domain in Orb2B (orb2BΔQ) and hence unable to form Orb2 complexes and long-term memory. We performed the IP and WB against the GFP tag to analyze the on/off kinetics of Orb2 protein expression using TARGET system. Orb2 protein is expressed at high levels within 3 or 4 hr after temperature shift to 27°C and is not detectable at the time of memory retrieval (Figure S2). Flies had fully rescued long-term memory both when kept at 27°C throughout adulthood and when shifted to 27°C for the duration of the training (Figure 6; Table S10) and only when a wild-type, but not the Q-domain-deleted, Orb2A was present. These results suggested that the Orb2A isoform and its Q domain are required in the MB neurons during memory acquisition for long-term memory formation.


Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Orb2A Is Required during Memory Acquisition and Orb2B during Memory Consolidation(Upper panel) Schematic of the rescue experiment with depicted domain organization of the proteins involved. (Lower panel) The plots indicate mean learning indices ± SEM of the temporal rescue of the orb2Orb2BΔQ and orb2mCPEB2RBD mutants with either UAS-orb2A/UAS-orb2AΔQ or UAS-orb2B/UAS-orb2BRBD∗ under control of MB247-Gal4 and tubGal80ts, trained and tested in single-pair assays with mated females for long-term memory and treated according to the regime outlined in the center of the panel. p values are for H0 LI = 0; ∗∗∗p < 0.001; ∗∗p < 0.01. See Table S10.
© Copyright Policy - CC BY
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4508346&req=5

fig6: Orb2A Is Required during Memory Acquisition and Orb2B during Memory Consolidation(Upper panel) Schematic of the rescue experiment with depicted domain organization of the proteins involved. (Lower panel) The plots indicate mean learning indices ± SEM of the temporal rescue of the orb2Orb2BΔQ and orb2mCPEB2RBD mutants with either UAS-orb2A/UAS-orb2AΔQ or UAS-orb2B/UAS-orb2BRBD∗ under control of MB247-Gal4 and tubGal80ts, trained and tested in single-pair assays with mated females for long-term memory and treated according to the regime outlined in the center of the panel. p values are for H0 LI = 0; ∗∗∗p < 0.001; ∗∗p < 0.01. See Table S10.
Mentions: To examine temporal requirement of Orb2A and its Q domain, we expressed in the MB neurons either wild-type Orb2A (UAS-orb2AGFP) or Orb2A with the Q domain deleted (UAS-orb2ΔQGFP) under control of MB247-Gal4 and temperature-gated tub-Gal80ts in the flies lacking the A isoform and the Q domain in Orb2B (orb2BΔQ) and hence unable to form Orb2 complexes and long-term memory. We performed the IP and WB against the GFP tag to analyze the on/off kinetics of Orb2 protein expression using TARGET system. Orb2 protein is expressed at high levels within 3 or 4 hr after temperature shift to 27°C and is not detectable at the time of memory retrieval (Figure S2). Flies had fully rescued long-term memory both when kept at 27°C throughout adulthood and when shifted to 27°C for the duration of the training (Figure 6; Table S10) and only when a wild-type, but not the Q-domain-deleted, Orb2A was present. These results suggested that the Orb2A isoform and its Q domain are required in the MB neurons during memory acquisition for long-term memory formation.

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus