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Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Orb2A Is Localized in Synapses of the MB γ LobeImmuno-EM of the orb2Orb2AGFP and orb2Orb2BGFP heterozygous brains. The sagittal sections of the brain in the region of the KC somata and the tip of the MB γ lobe were analyzed. (i) Orb2AGFP is absent from the neuronal cell bodies (arrow) of the Kenyon cells. (ii) Orb2AGFP labeled by DAB precipitates is present in T-bars (asterisk) and active zones (double asterisk) in the MB γ lobe synapses. (iii) Orb2BGFP labeled by DAB precipitates is detected in the KC cell bodies including T-bars (asterisk) and active zones (double asterisk). (iv) Orb2BGFP labeled by DAB precipitates is present in the MB γ lobe including T-bars (asterisk). In all panels, scale bars represent 500 nm.
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fig5: Orb2A Is Localized in Synapses of the MB γ LobeImmuno-EM of the orb2Orb2AGFP and orb2Orb2BGFP heterozygous brains. The sagittal sections of the brain in the region of the KC somata and the tip of the MB γ lobe were analyzed. (i) Orb2AGFP is absent from the neuronal cell bodies (arrow) of the Kenyon cells. (ii) Orb2AGFP labeled by DAB precipitates is present in T-bars (asterisk) and active zones (double asterisk) in the MB γ lobe synapses. (iii) Orb2BGFP labeled by DAB precipitates is detected in the KC cell bodies including T-bars (asterisk) and active zones (double asterisk). (iv) Orb2BGFP labeled by DAB precipitates is present in the MB γ lobe including T-bars (asterisk). In all panels, scale bars represent 500 nm.

Mentions: Using immuno-electron microscopy against the GFP tag on the endogenous Orb2A and Orb2B proteins encoded by orbOrb2AGFP and orbOrb2BGFP, respectively, we determined their subcellular localization (Krüttner et al., 2012). We examined KC somata and the output region of the MB, tip of the γ lobe, innervated by the aSP13 neurons in the brains of viable heterozygous orbOrb2AGFP and orbOrb2BGFP flies. We detected Orb2A protein present in a pattern distinct to that of the Orb2B protein. Orb2B is broadly expressed in the KC cell bodies and axons of the γ neurons, including synapses, whereas Orb2A is excluded from the neuronal cell bodies and is almost exclusively present in synapses of the MB γ lobe (Figure 5). These results imply that Orb2 isoforms, previously shown to function in long-term memory through distinct mechanisms (Krüttner et al., 2012), likely play distinct roles in long-term memory.


Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Orb2A Is Localized in Synapses of the MB γ LobeImmuno-EM of the orb2Orb2AGFP and orb2Orb2BGFP heterozygous brains. The sagittal sections of the brain in the region of the KC somata and the tip of the MB γ lobe were analyzed. (i) Orb2AGFP is absent from the neuronal cell bodies (arrow) of the Kenyon cells. (ii) Orb2AGFP labeled by DAB precipitates is present in T-bars (asterisk) and active zones (double asterisk) in the MB γ lobe synapses. (iii) Orb2BGFP labeled by DAB precipitates is detected in the KC cell bodies including T-bars (asterisk) and active zones (double asterisk). (iv) Orb2BGFP labeled by DAB precipitates is present in the MB γ lobe including T-bars (asterisk). In all panels, scale bars represent 500 nm.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508346&req=5

fig5: Orb2A Is Localized in Synapses of the MB γ LobeImmuno-EM of the orb2Orb2AGFP and orb2Orb2BGFP heterozygous brains. The sagittal sections of the brain in the region of the KC somata and the tip of the MB γ lobe were analyzed. (i) Orb2AGFP is absent from the neuronal cell bodies (arrow) of the Kenyon cells. (ii) Orb2AGFP labeled by DAB precipitates is present in T-bars (asterisk) and active zones (double asterisk) in the MB γ lobe synapses. (iii) Orb2BGFP labeled by DAB precipitates is detected in the KC cell bodies including T-bars (asterisk) and active zones (double asterisk). (iv) Orb2BGFP labeled by DAB precipitates is present in the MB γ lobe including T-bars (asterisk). In all panels, scale bars represent 500 nm.
Mentions: Using immuno-electron microscopy against the GFP tag on the endogenous Orb2A and Orb2B proteins encoded by orbOrb2AGFP and orbOrb2BGFP, respectively, we determined their subcellular localization (Krüttner et al., 2012). We examined KC somata and the output region of the MB, tip of the γ lobe, innervated by the aSP13 neurons in the brains of viable heterozygous orbOrb2AGFP and orbOrb2BGFP flies. We detected Orb2A protein present in a pattern distinct to that of the Orb2B protein. Orb2B is broadly expressed in the KC cell bodies and axons of the γ neurons, including synapses, whereas Orb2A is excluded from the neuronal cell bodies and is almost exclusively present in synapses of the MB γ lobe (Figure 5). These results imply that Orb2 isoforms, previously shown to function in long-term memory through distinct mechanisms (Krüttner et al., 2012), likely play distinct roles in long-term memory.

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus