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Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Orb2 Mediates Long-Term Memory Consolidation Downstream of DopR1(A) Orb2 is required for dopamine-mediated memory consolidation. The plots indicate mean learning indices ± SEM of males of the indicated genotypes tested in single-pair assays with mated females 24 hr after being starved for 16 hr, trained with a mated female for 1 hr, and fed with dopamine. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S8.(B) DopR1 mediates Orb2 oligomer formation. Head extracts from adult flies of the indicated genotypes were analyzed by IP and WB for presence of the Orb2GFP complexes, after being starved for 16 hr and fed with dopamine as indicated.(C) WB signal (from C), corresponding to the Orb2-GFP oligomers, has been quantified using Fiji-ImageJ. The values on y axis represent the mean intensity normalized to the wild-type not treated with dopamine (wt − dopamine). See Table S9.
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fig4: Orb2 Mediates Long-Term Memory Consolidation Downstream of DopR1(A) Orb2 is required for dopamine-mediated memory consolidation. The plots indicate mean learning indices ± SEM of males of the indicated genotypes tested in single-pair assays with mated females 24 hr after being starved for 16 hr, trained with a mated female for 1 hr, and fed with dopamine. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S8.(B) DopR1 mediates Orb2 oligomer formation. Head extracts from adult flies of the indicated genotypes were analyzed by IP and WB for presence of the Orb2GFP complexes, after being starved for 16 hr and fed with dopamine as indicated.(C) WB signal (from C), corresponding to the Orb2-GFP oligomers, has been quantified using Fiji-ImageJ. The values on y axis represent the mean intensity normalized to the wild-type not treated with dopamine (wt − dopamine). See Table S9.

Mentions: Feeding animals with dopamine activates dopaminergic pathways globally, which leads to formation of Orb2 protein complexes consisting of Orb2A and Orb2B. The Orb2 complex correlates strictly with the ability of males to form courtship long-term memory (Krüttner et al., 2012; Majumdar et al., 2012). To test whether Orb2 complexes are required for dopamine-mediated memory consolidation, we used an endogenously modified orb2 mutant allele. This mutant lacks the Q domain (orb2ΔQ) and has been previously shown to be dispensable for short-term memory but critical for both Orb2 complex formation and the maintenance of the courtship memory after 6 hr (Keleman et al., 2007; Krüttner et al., 2012). orb2ΔQ mutants were unable to consolidate short-term memory into a memory lasting 24 hr upon feeding with dopamine, in comparison to the animals bearing the wild-type allele (orb2+; Figure 4A; Table S8).


Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Orb2 Mediates Long-Term Memory Consolidation Downstream of DopR1(A) Orb2 is required for dopamine-mediated memory consolidation. The plots indicate mean learning indices ± SEM of males of the indicated genotypes tested in single-pair assays with mated females 24 hr after being starved for 16 hr, trained with a mated female for 1 hr, and fed with dopamine. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S8.(B) DopR1 mediates Orb2 oligomer formation. Head extracts from adult flies of the indicated genotypes were analyzed by IP and WB for presence of the Orb2GFP complexes, after being starved for 16 hr and fed with dopamine as indicated.(C) WB signal (from C), corresponding to the Orb2-GFP oligomers, has been quantified using Fiji-ImageJ. The values on y axis represent the mean intensity normalized to the wild-type not treated with dopamine (wt − dopamine). See Table S9.
© Copyright Policy - CC BY
Related In: Results  -  Collection

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fig4: Orb2 Mediates Long-Term Memory Consolidation Downstream of DopR1(A) Orb2 is required for dopamine-mediated memory consolidation. The plots indicate mean learning indices ± SEM of males of the indicated genotypes tested in single-pair assays with mated females 24 hr after being starved for 16 hr, trained with a mated female for 1 hr, and fed with dopamine. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S8.(B) DopR1 mediates Orb2 oligomer formation. Head extracts from adult flies of the indicated genotypes were analyzed by IP and WB for presence of the Orb2GFP complexes, after being starved for 16 hr and fed with dopamine as indicated.(C) WB signal (from C), corresponding to the Orb2-GFP oligomers, has been quantified using Fiji-ImageJ. The values on y axis represent the mean intensity normalized to the wild-type not treated with dopamine (wt − dopamine). See Table S9.
Mentions: Feeding animals with dopamine activates dopaminergic pathways globally, which leads to formation of Orb2 protein complexes consisting of Orb2A and Orb2B. The Orb2 complex correlates strictly with the ability of males to form courtship long-term memory (Krüttner et al., 2012; Majumdar et al., 2012). To test whether Orb2 complexes are required for dopamine-mediated memory consolidation, we used an endogenously modified orb2 mutant allele. This mutant lacks the Q domain (orb2ΔQ) and has been previously shown to be dispensable for short-term memory but critical for both Orb2 complex formation and the maintenance of the courtship memory after 6 hr (Keleman et al., 2007; Krüttner et al., 2012). orb2ΔQ mutants were unable to consolidate short-term memory into a memory lasting 24 hr upon feeding with dopamine, in comparison to the animals bearing the wild-type allele (orb2+; Figure 4A; Table S8).

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus