Limits...
Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus

DopR1 Is Necessary in the MB γ Neurons for Long-Term Memory Consolidation(A) Post-acquisition inactivation of DopR1 impairs long-term memory. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 7 hr with a mated female and fed with SCH23390 for 6 hr if indicated. p values are for H0 LI = 0; ∗∗∗p < 0.001; ∗∗p < 0.01. See Table S5.(B) DopR1 is required for dopamine-mediated memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being starved for 16 hr, trained for 1 hr with a mated female, and fed with dopamine and SCH23390 as indicated between training and test. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S6.(C) DopR1 functions in long-term memory in the MB γ neurons. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 7 hr with a mated female. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S7.The plots indicate mean learning indices ± SEM.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4508346&req=5

fig3: DopR1 Is Necessary in the MB γ Neurons for Long-Term Memory Consolidation(A) Post-acquisition inactivation of DopR1 impairs long-term memory. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 7 hr with a mated female and fed with SCH23390 for 6 hr if indicated. p values are for H0 LI = 0; ∗∗∗p < 0.001; ∗∗p < 0.01. See Table S5.(B) DopR1 is required for dopamine-mediated memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being starved for 16 hr, trained for 1 hr with a mated female, and fed with dopamine and SCH23390 as indicated between training and test. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S6.(C) DopR1 functions in long-term memory in the MB γ neurons. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 7 hr with a mated female. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S7.The plots indicate mean learning indices ± SEM.

Mentions: Males lacking DopR1 were unable to form long-term courtship memory after 7 hr of training. In contrast, DopR2 mutants displayed normal long-term memory (Figure 3A; Table S5). To confirm that long-term memory deficit in the DopR1 mutants is indeed due to loss of DopR1 function, we analyzed DopR1res flies where the deleted genomic region was reintegrated by site-specific transgenesis (Keleman et al., 2012). These flies performed just as well as wild-type animals, suggesting that DopR1, but not DopR2, is required for long-term memory (Figure 3A; Table S5). However, given that DopR1 receptor is required for acquisition of the courtship short-term memory (Keleman et al., 2012), the impairment of long-term memory in DopR1 mutants might be due to its involvement in this early phase of memory formation: thus, these results alone do not prove DopR1’s role in memory consolidation. To address explicitly the requirement of DopR1 in long-term memory after memory acquisition, we fed wild-type flies and DopR2attp mutants (lacking DopR2, but not DopR1) after 7-hr training for long-term memory with the antagonist (SCH23390) specific for both receptors (Gotzes et al., 1994). These flies did not form long-term memory in contrast to animals that were not fed with the antagonist, suggesting that DopR1 has a post-acquisition role in long-term memory (Figure 3A; Table S5).


Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

DopR1 Is Necessary in the MB γ Neurons for Long-Term Memory Consolidation(A) Post-acquisition inactivation of DopR1 impairs long-term memory. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 7 hr with a mated female and fed with SCH23390 for 6 hr if indicated. p values are for H0 LI = 0; ∗∗∗p < 0.001; ∗∗p < 0.01. See Table S5.(B) DopR1 is required for dopamine-mediated memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being starved for 16 hr, trained for 1 hr with a mated female, and fed with dopamine and SCH23390 as indicated between training and test. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S6.(C) DopR1 functions in long-term memory in the MB γ neurons. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 7 hr with a mated female. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S7.The plots indicate mean learning indices ± SEM.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508346&req=5

fig3: DopR1 Is Necessary in the MB γ Neurons for Long-Term Memory Consolidation(A) Post-acquisition inactivation of DopR1 impairs long-term memory. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 7 hr with a mated female and fed with SCH23390 for 6 hr if indicated. p values are for H0 LI = 0; ∗∗∗p < 0.001; ∗∗p < 0.01. See Table S5.(B) DopR1 is required for dopamine-mediated memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being starved for 16 hr, trained for 1 hr with a mated female, and fed with dopamine and SCH23390 as indicated between training and test. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S6.(C) DopR1 functions in long-term memory in the MB γ neurons. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 7 hr with a mated female. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S7.The plots indicate mean learning indices ± SEM.
Mentions: Males lacking DopR1 were unable to form long-term courtship memory after 7 hr of training. In contrast, DopR2 mutants displayed normal long-term memory (Figure 3A; Table S5). To confirm that long-term memory deficit in the DopR1 mutants is indeed due to loss of DopR1 function, we analyzed DopR1res flies where the deleted genomic region was reintegrated by site-specific transgenesis (Keleman et al., 2012). These flies performed just as well as wild-type animals, suggesting that DopR1, but not DopR2, is required for long-term memory (Figure 3A; Table S5). However, given that DopR1 receptor is required for acquisition of the courtship short-term memory (Keleman et al., 2012), the impairment of long-term memory in DopR1 mutants might be due to its involvement in this early phase of memory formation: thus, these results alone do not prove DopR1’s role in memory consolidation. To address explicitly the requirement of DopR1 in long-term memory after memory acquisition, we fed wild-type flies and DopR2attp mutants (lacking DopR2, but not DopR1) after 7-hr training for long-term memory with the antagonist (SCH23390) specific for both receptors (Gotzes et al., 1994). These flies did not form long-term memory in contrast to animals that were not fed with the antagonist, suggesting that DopR1 has a post-acquisition role in long-term memory (Figure 3A; Table S5).

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus