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Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Subset of the PAM-DA Neurons, aSP13, Consolidates Short-Term Memory into Long-Term Memory in a Protein-Synthesis-Dependent Manner(A) Post-acquisition activation of the DA neurons mediates protein-synthesis-dependent memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 1 hr with a mated female and warmed at 32°C for 2 hr at the indicated time points (i); experimental control males, which stayed at 22°C all the time; and the genetic control animals, which were warmed at 32°C for 2 hr between 8 and 10 hr after learning (ii); and males fed with the cycloheximide during activation with TrpA1 between 8 and 10 hr after 1 hr training (iii). p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S2.(B) Subset of the aSP13-DA neurons is sufficient for the long-term memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after training for 1 hr with a mated female and being warmed at 32°C (except control males which stayed at 22°C) between 8 and 10 hr after training. p values are for H0 LI = 0; ∗∗p < 0.01; ∗∗∗p < 0.001. See Table S3.(C) Post-acquisition silencing of the aSP13-DA neurons prevents long-term memory formation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after training for 7 hr with a mated female and being warmed at 32°C (except control males, which stayed at 22°C) between 8 and 11 hr after training. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S4.The plots indicate mean learning indices ± SEM.
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fig2: Subset of the PAM-DA Neurons, aSP13, Consolidates Short-Term Memory into Long-Term Memory in a Protein-Synthesis-Dependent Manner(A) Post-acquisition activation of the DA neurons mediates protein-synthesis-dependent memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 1 hr with a mated female and warmed at 32°C for 2 hr at the indicated time points (i); experimental control males, which stayed at 22°C all the time; and the genetic control animals, which were warmed at 32°C for 2 hr between 8 and 10 hr after learning (ii); and males fed with the cycloheximide during activation with TrpA1 between 8 and 10 hr after 1 hr training (iii). p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S2.(B) Subset of the aSP13-DA neurons is sufficient for the long-term memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after training for 1 hr with a mated female and being warmed at 32°C (except control males which stayed at 22°C) between 8 and 10 hr after training. p values are for H0 LI = 0; ∗∗p < 0.01; ∗∗∗p < 0.001. See Table S3.(C) Post-acquisition silencing of the aSP13-DA neurons prevents long-term memory formation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after training for 7 hr with a mated female and being warmed at 32°C (except control males, which stayed at 22°C) between 8 and 11 hr after training. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S4.The plots indicate mean learning indices ± SEM.

Mentions: Males expressing TrpA1 were incubated at 32°C for 2 hr at various time points after 1-hr training with a mated female. Flies, which were incubated at 32°C 8–10 hr after training fully consolidated short-term memory into long-term memory, in contrast to appropriate genetic control flies or flies that were switched to 32°C at other time points or control flies that remained at 22°C throughout (Figures 2Ai and 2Aii; Table S2). Importantly, this consolidated form of memory was dependent on de novo protein synthesis, because feeding the males with the protein synthesis inhibitor cycloheximide prevented the PAM-DA-stimulation-induced long-lasting memory (Figure 2Aiii; Table S2). Given our previous results showing that PAM-DA neurons are necessary for short-term memory acquisition, these results suggest that delayed activation of the same PAM-DA neurons between 8 and 10 hr after training is sufficient to consolidate short-term memory into a protein-synthesis-dependent long-term memory.


Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Subset of the PAM-DA Neurons, aSP13, Consolidates Short-Term Memory into Long-Term Memory in a Protein-Synthesis-Dependent Manner(A) Post-acquisition activation of the DA neurons mediates protein-synthesis-dependent memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 1 hr with a mated female and warmed at 32°C for 2 hr at the indicated time points (i); experimental control males, which stayed at 22°C all the time; and the genetic control animals, which were warmed at 32°C for 2 hr between 8 and 10 hr after learning (ii); and males fed with the cycloheximide during activation with TrpA1 between 8 and 10 hr after 1 hr training (iii). p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S2.(B) Subset of the aSP13-DA neurons is sufficient for the long-term memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after training for 1 hr with a mated female and being warmed at 32°C (except control males which stayed at 22°C) between 8 and 10 hr after training. p values are for H0 LI = 0; ∗∗p < 0.01; ∗∗∗p < 0.001. See Table S3.(C) Post-acquisition silencing of the aSP13-DA neurons prevents long-term memory formation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after training for 7 hr with a mated female and being warmed at 32°C (except control males, which stayed at 22°C) between 8 and 11 hr after training. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S4.The plots indicate mean learning indices ± SEM.
© Copyright Policy - CC BY
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4508346&req=5

fig2: Subset of the PAM-DA Neurons, aSP13, Consolidates Short-Term Memory into Long-Term Memory in a Protein-Synthesis-Dependent Manner(A) Post-acquisition activation of the DA neurons mediates protein-synthesis-dependent memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after being trained for 1 hr with a mated female and warmed at 32°C for 2 hr at the indicated time points (i); experimental control males, which stayed at 22°C all the time; and the genetic control animals, which were warmed at 32°C for 2 hr between 8 and 10 hr after learning (ii); and males fed with the cycloheximide during activation with TrpA1 between 8 and 10 hr after 1 hr training (iii). p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S2.(B) Subset of the aSP13-DA neurons is sufficient for the long-term memory consolidation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after training for 1 hr with a mated female and being warmed at 32°C (except control males which stayed at 22°C) between 8 and 10 hr after training. p values are for H0 LI = 0; ∗∗p < 0.01; ∗∗∗p < 0.001. See Table S3.(C) Post-acquisition silencing of the aSP13-DA neurons prevents long-term memory formation. Males of the indicated genotypes were tested in single-pair assays with mated females 24 hr after training for 7 hr with a mated female and being warmed at 32°C (except control males, which stayed at 22°C) between 8 and 11 hr after training. p values are for H0 LI = 0; ∗∗∗p < 0.001. See Table S4.The plots indicate mean learning indices ± SEM.
Mentions: Males expressing TrpA1 were incubated at 32°C for 2 hr at various time points after 1-hr training with a mated female. Flies, which were incubated at 32°C 8–10 hr after training fully consolidated short-term memory into long-term memory, in contrast to appropriate genetic control flies or flies that were switched to 32°C at other time points or control flies that remained at 22°C throughout (Figures 2Ai and 2Aii; Table S2). Importantly, this consolidated form of memory was dependent on de novo protein synthesis, because feeding the males with the protein synthesis inhibitor cycloheximide prevented the PAM-DA-stimulation-induced long-lasting memory (Figure 2Aiii; Table S2). Given our previous results showing that PAM-DA neurons are necessary for short-term memory acquisition, these results suggest that delayed activation of the same PAM-DA neurons between 8 and 10 hr after training is sufficient to consolidate short-term memory into a protein-synthesis-dependent long-term memory.

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus