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Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Post-learning Global Activation of Dopamine Pathways Is Sufficient to Consolidate Short-Term Memory into Long-Term MemoryThe plots indicate mean learning indices ± SEM of the wild-type Canton-S males tested in single-pair assays with mated or virgin females, either 24 hr after being starved for 16 hr, trained with mated female for 1 hr, and fed with dopamine for 23 hr (if indicated; LTM; dark green bars) or immediately after training (STM; light green bar). p values are for H0 LI = 0; ∗p < 0.05; ∗∗∗p < 0.001. See Table S1.
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fig1: Post-learning Global Activation of Dopamine Pathways Is Sufficient to Consolidate Short-Term Memory into Long-Term MemoryThe plots indicate mean learning indices ± SEM of the wild-type Canton-S males tested in single-pair assays with mated or virgin females, either 24 hr after being starved for 16 hr, trained with mated female for 1 hr, and fed with dopamine for 23 hr (if indicated; LTM; dark green bars) or immediately after training (STM; light green bar). p values are for H0 LI = 0; ∗p < 0.05; ∗∗∗p < 0.001. See Table S1.

Mentions: We employed a paradigm for courtship memory consolidation to investigate the molecular basis and spatiotemporal relationships between the two key processes of long-term memory formation, memory acquisition and its consolidation, in the Drosophila male. We combined a learning experience sufficient to establish courtship short-term memory, but not long-term memory, with a subsequent stimulation of the dopaminergic pathways, which is thought to induce local protein synthesis at synapses (Smith et al., 2005). We starved naive males for 16 hr, trained them for 1 hr with mated female, and then activated dopamine pathways globally by feeding the animals with dopamine for 23 hr (Riemensperger et al., 2011). This resulted in robust long-term memory, in the form of a strong suppression of their courtship toward mated females in comparison to virgin females when tested 24 hr later. This memory was quantified as a learning index (LI), which measures the extent of the courtship suppression (Figure 1; Table S1). By contrast, training for 1 hr alone induced normal short-term memory, but not long-term memory, and dopaminergic activation alone did not induce long-term memory. Thus post-acquisition dopamine stimulation consolidates short-term memory into long-term memory.


Synaptic Orb2A Bridges Memory Acquisition and Late Memory Consolidation in Drosophila.

Krüttner S, Traunmüller L, Dag U, Jandrasits K, Stepien B, Iyer N, Fradkin LG, Noordermeer JN, Mensh BD, Keleman K - Cell Rep (2015)

Post-learning Global Activation of Dopamine Pathways Is Sufficient to Consolidate Short-Term Memory into Long-Term MemoryThe plots indicate mean learning indices ± SEM of the wild-type Canton-S males tested in single-pair assays with mated or virgin females, either 24 hr after being starved for 16 hr, trained with mated female for 1 hr, and fed with dopamine for 23 hr (if indicated; LTM; dark green bars) or immediately after training (STM; light green bar). p values are for H0 LI = 0; ∗p < 0.05; ∗∗∗p < 0.001. See Table S1.
© Copyright Policy - CC BY
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4508346&req=5

fig1: Post-learning Global Activation of Dopamine Pathways Is Sufficient to Consolidate Short-Term Memory into Long-Term MemoryThe plots indicate mean learning indices ± SEM of the wild-type Canton-S males tested in single-pair assays with mated or virgin females, either 24 hr after being starved for 16 hr, trained with mated female for 1 hr, and fed with dopamine for 23 hr (if indicated; LTM; dark green bars) or immediately after training (STM; light green bar). p values are for H0 LI = 0; ∗p < 0.05; ∗∗∗p < 0.001. See Table S1.
Mentions: We employed a paradigm for courtship memory consolidation to investigate the molecular basis and spatiotemporal relationships between the two key processes of long-term memory formation, memory acquisition and its consolidation, in the Drosophila male. We combined a learning experience sufficient to establish courtship short-term memory, but not long-term memory, with a subsequent stimulation of the dopaminergic pathways, which is thought to induce local protein synthesis at synapses (Smith et al., 2005). We starved naive males for 16 hr, trained them for 1 hr with mated female, and then activated dopamine pathways globally by feeding the animals with dopamine for 23 hr (Riemensperger et al., 2011). This resulted in robust long-term memory, in the form of a strong suppression of their courtship toward mated females in comparison to virgin females when tested 24 hr later. This memory was quantified as a learning index (LI), which measures the extent of the courtship suppression (Figure 1; Table S1). By contrast, training for 1 hr alone induced normal short-term memory, but not long-term memory, and dopaminergic activation alone did not induce long-term memory. Thus post-acquisition dopamine stimulation consolidates short-term memory into long-term memory.

Bottom Line: Furthermore, we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation.Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII.Thus, our results provide evidence for the role of a neuromodulated, synapse-restricted molecule bridging memory acquisition and long-term memory consolidation in a learning animal.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Molecular Pathology, Doktor Bohr Gasse 7, A-1030 Vienna, Austria; Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus