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An Association Study on IL16 Gene Polymorphisms with the Risk of Sporadic Alzheimer's Disease.

Khoshbakht T, Soosanabadi M, Neishaboury M, Kamali K, Karimlou M, Bazazzadegan N, Khorram Khorshid HR - Avicenna J Med Biotechnol (2015 Jul-Sep)

Bottom Line: Genotyping of rs11556218 T/G and rs4778889 T/C polymorphisms was performed by PCR-RFLP method using the NdeI and AhdI restriction enzymes, respectively.However, there was no significant difference in the frequencies of rs4778889 alleles between the AD patients and controls.Our results indicate that the rs11556218 and rs4778889 polymorphisms have a protective role in the development of sporadic AD in Iranian population.

View Article: PubMed Central - PubMed

Affiliation: Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.

ABSTRACT

Background: Interleukin-16 (IL-16) is an important regulator of T cell activation and was reported to act as a chemoattractant agent. There are evidences that IL16 can control the neuroinflammatory processes in Alzheimer's Disease (AD). This study was performed to investigate the role or association of IL16 polymorphisms, rs11556218 and rs4778889 with the risk of late-onset Alzheimer's disease (LOAD) in Iranian population.

Methods: Totally, 148 AD patients and 137 nondemented and age-matched subjects were recruited in this study. Genotyping of rs11556218 T/G and rs4778889 T/C polymorphisms was performed by PCR-RFLP method using the NdeI and AhdI restriction enzymes, respectively.

Results: Statistical analysis of rs11556218 genotypes showed a protective effect against AD in the heterozygote genotype (p=0.001, OR=0.16) as well as rs4778889 (p=0.001, OR=0.23). Frequency of rs11556218 allele T was higher in controls than patients (p=0.001, OR=0.32). However, there was no significant difference in the frequencies of rs4778889 alleles between the AD patients and controls.

Conclusion: Our results indicate that the rs11556218 and rs4778889 polymorphisms have a protective role in the development of sporadic AD in Iranian population.

No MeSH data available.


Related in: MedlinePlus

PCR- RFLP profile of IL16 rs4778889 T/C polymorphism digested with AhdI restriction enzyme.
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Figure 2: PCR- RFLP profile of IL16 rs4778889 T/C polymorphism digested with AhdI restriction enzyme.

Mentions: The PCR products of IL16 rs11556218 T/G and IL16 rs4778889 T/C were digested with restriction enzymes NdeI and AhdI at 37 °C overnight, respectively. DNA fragments were subjected to 8% polyacrylamide gel electrophoresis and stained with silver nitrate. Based on figure 1, the G allele of the rs11556218 produced two fragments 147 and 24 bp, whereas the T allele remained undigested (171 bp). In IL16 rs4778889 T/C variant, the C allele was cut into two fragments 146 and 34 bp, while the T allele remained uncut (Figure 2).


An Association Study on IL16 Gene Polymorphisms with the Risk of Sporadic Alzheimer's Disease.

Khoshbakht T, Soosanabadi M, Neishaboury M, Kamali K, Karimlou M, Bazazzadegan N, Khorram Khorshid HR - Avicenna J Med Biotechnol (2015 Jul-Sep)

PCR- RFLP profile of IL16 rs4778889 T/C polymorphism digested with AhdI restriction enzyme.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508336&req=5

Figure 2: PCR- RFLP profile of IL16 rs4778889 T/C polymorphism digested with AhdI restriction enzyme.
Mentions: The PCR products of IL16 rs11556218 T/G and IL16 rs4778889 T/C were digested with restriction enzymes NdeI and AhdI at 37 °C overnight, respectively. DNA fragments were subjected to 8% polyacrylamide gel electrophoresis and stained with silver nitrate. Based on figure 1, the G allele of the rs11556218 produced two fragments 147 and 24 bp, whereas the T allele remained undigested (171 bp). In IL16 rs4778889 T/C variant, the C allele was cut into two fragments 146 and 34 bp, while the T allele remained uncut (Figure 2).

Bottom Line: Genotyping of rs11556218 T/G and rs4778889 T/C polymorphisms was performed by PCR-RFLP method using the NdeI and AhdI restriction enzymes, respectively.However, there was no significant difference in the frequencies of rs4778889 alleles between the AD patients and controls.Our results indicate that the rs11556218 and rs4778889 polymorphisms have a protective role in the development of sporadic AD in Iranian population.

View Article: PubMed Central - PubMed

Affiliation: Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.

ABSTRACT

Background: Interleukin-16 (IL-16) is an important regulator of T cell activation and was reported to act as a chemoattractant agent. There are evidences that IL16 can control the neuroinflammatory processes in Alzheimer's Disease (AD). This study was performed to investigate the role or association of IL16 polymorphisms, rs11556218 and rs4778889 with the risk of late-onset Alzheimer's disease (LOAD) in Iranian population.

Methods: Totally, 148 AD patients and 137 nondemented and age-matched subjects were recruited in this study. Genotyping of rs11556218 T/G and rs4778889 T/C polymorphisms was performed by PCR-RFLP method using the NdeI and AhdI restriction enzymes, respectively.

Results: Statistical analysis of rs11556218 genotypes showed a protective effect against AD in the heterozygote genotype (p=0.001, OR=0.16) as well as rs4778889 (p=0.001, OR=0.23). Frequency of rs11556218 allele T was higher in controls than patients (p=0.001, OR=0.32). However, there was no significant difference in the frequencies of rs4778889 alleles between the AD patients and controls.

Conclusion: Our results indicate that the rs11556218 and rs4778889 polymorphisms have a protective role in the development of sporadic AD in Iranian population.

No MeSH data available.


Related in: MedlinePlus