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Retinoic Acid as the Stimulating Factor for Differentiation of Wharton's Jelly-Mesenchymal Stem Cells into Hepatocyte-like Cells.

Mortezaee K, Minaii B, Sabbaghziarani F, Ragerdi Kashani I, Hassanzadeh G, Pasbakhsh P, Barbarestani M, Latifpour M - Avicenna J Med Biotechnol (2015 Jul-Sep)

Bottom Line: WJ-MSCs expressed high levels of CD90 (93.6%) and CD105 (90.7%), but low levels of CD34 (0.3%) and CD45 (0.8%).Albumin production had significant difference in the two groups (p≤0.05).The data showed specific characteristics in favor of considering the differentiated cells as hepatocyte-like cells such as obtaining morphologic, functional, and αFP and HNF1-α expression patterns which in turn were higher in cells exposed to RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Wharton's Jelly-Mesenchymal Stem Cells (WJ-MSCs) are pluripotent cells with differentiation capability into most cell lineages. The aim of the current work was to examine the role of Retinoic Acid (RA) in differentiation process of these cells into hepatocyte-like cells and determine the morphological and functional patterns.

Methods: Human WJ-MSCs were extracted, cultured and expanded; after approximately 95% of confluence, the cells were treated with hepatogenic media containing RA. The cells were subsequently analyzed for morphological changes, glycogen storage, albumin production, and specific gene expression.

Results: WJ-MSCs expressed high levels of CD90 (93.6%) and CD105 (90.7%), but low levels of CD34 (0.3%) and CD45 (0.8%). Albumin production had significant difference in the two groups (p≤0.05). The data showed specific characteristics in favor of considering the differentiated cells as hepatocyte-like cells such as obtaining morphologic, functional, and αFP and HNF1-α expression patterns which in turn were higher in cells exposed to RA.

Conclusion: Based on the data of present study, RA is an effective molecule in inducing differentiation of WJ-MSCs into hepatocyte-like cells; therefore, it may be considered as a promising factor for targeting therapy of liver disorders.

No MeSH data available.


Related in: MedlinePlus

Expression pattern of hepatocyte-like cells specific markers. It is clear from the figure that the highest expression level for αFP was at day 14, but for HNF1-α at day 28. RA had the profound effects on expression levels of the two discussed markers. We can also see that the rates for cells at day 0 were not significant. WJ= Wharton's jelly-mesenchymal stem cells; αFP=alpha-fetoprotein; and HNF-1α=hepatocyte nuclear factor-1α.
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Figure 4: Expression pattern of hepatocyte-like cells specific markers. It is clear from the figure that the highest expression level for αFP was at day 14, but for HNF1-α at day 28. RA had the profound effects on expression levels of the two discussed markers. We can also see that the rates for cells at day 0 were not significant. WJ= Wharton's jelly-mesenchymal stem cells; αFP=alpha-fetoprotein; and HNF-1α=hepatocyte nuclear factor-1α.

Mentions: Expression patterns of αFP and HNF1-α were analyzed in hepatocyte-like cells using RT-PCR technique. The rate of αFP expression, as the early marker, in cells exposed to RA was the highest on day 14 with a downside trend over time. On the other hand, the rate for HNF1-α, as the secondary marker, was highest in RA receiving cells on day 28; however, its expression was less during the first two weeks with or without RA (Figure 4).


Retinoic Acid as the Stimulating Factor for Differentiation of Wharton's Jelly-Mesenchymal Stem Cells into Hepatocyte-like Cells.

Mortezaee K, Minaii B, Sabbaghziarani F, Ragerdi Kashani I, Hassanzadeh G, Pasbakhsh P, Barbarestani M, Latifpour M - Avicenna J Med Biotechnol (2015 Jul-Sep)

Expression pattern of hepatocyte-like cells specific markers. It is clear from the figure that the highest expression level for αFP was at day 14, but for HNF1-α at day 28. RA had the profound effects on expression levels of the two discussed markers. We can also see that the rates for cells at day 0 were not significant. WJ= Wharton's jelly-mesenchymal stem cells; αFP=alpha-fetoprotein; and HNF-1α=hepatocyte nuclear factor-1α.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508333&req=5

Figure 4: Expression pattern of hepatocyte-like cells specific markers. It is clear from the figure that the highest expression level for αFP was at day 14, but for HNF1-α at day 28. RA had the profound effects on expression levels of the two discussed markers. We can also see that the rates for cells at day 0 were not significant. WJ= Wharton's jelly-mesenchymal stem cells; αFP=alpha-fetoprotein; and HNF-1α=hepatocyte nuclear factor-1α.
Mentions: Expression patterns of αFP and HNF1-α were analyzed in hepatocyte-like cells using RT-PCR technique. The rate of αFP expression, as the early marker, in cells exposed to RA was the highest on day 14 with a downside trend over time. On the other hand, the rate for HNF1-α, as the secondary marker, was highest in RA receiving cells on day 28; however, its expression was less during the first two weeks with or without RA (Figure 4).

Bottom Line: WJ-MSCs expressed high levels of CD90 (93.6%) and CD105 (90.7%), but low levels of CD34 (0.3%) and CD45 (0.8%).Albumin production had significant difference in the two groups (p≤0.05).The data showed specific characteristics in favor of considering the differentiated cells as hepatocyte-like cells such as obtaining morphologic, functional, and αFP and HNF1-α expression patterns which in turn were higher in cells exposed to RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Wharton's Jelly-Mesenchymal Stem Cells (WJ-MSCs) are pluripotent cells with differentiation capability into most cell lineages. The aim of the current work was to examine the role of Retinoic Acid (RA) in differentiation process of these cells into hepatocyte-like cells and determine the morphological and functional patterns.

Methods: Human WJ-MSCs were extracted, cultured and expanded; after approximately 95% of confluence, the cells were treated with hepatogenic media containing RA. The cells were subsequently analyzed for morphological changes, glycogen storage, albumin production, and specific gene expression.

Results: WJ-MSCs expressed high levels of CD90 (93.6%) and CD105 (90.7%), but low levels of CD34 (0.3%) and CD45 (0.8%). Albumin production had significant difference in the two groups (p≤0.05). The data showed specific characteristics in favor of considering the differentiated cells as hepatocyte-like cells such as obtaining morphologic, functional, and αFP and HNF1-α expression patterns which in turn were higher in cells exposed to RA.

Conclusion: Based on the data of present study, RA is an effective molecule in inducing differentiation of WJ-MSCs into hepatocyte-like cells; therefore, it may be considered as a promising factor for targeting therapy of liver disorders.

No MeSH data available.


Related in: MedlinePlus