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Retinoic Acid as the Stimulating Factor for Differentiation of Wharton's Jelly-Mesenchymal Stem Cells into Hepatocyte-like Cells.

Mortezaee K, Minaii B, Sabbaghziarani F, Ragerdi Kashani I, Hassanzadeh G, Pasbakhsh P, Barbarestani M, Latifpour M - Avicenna J Med Biotechnol (2015 Jul-Sep)

Bottom Line: WJ-MSCs expressed high levels of CD90 (93.6%) and CD105 (90.7%), but low levels of CD34 (0.3%) and CD45 (0.8%).Albumin production had significant difference in the two groups (p≤0.05).The data showed specific characteristics in favor of considering the differentiated cells as hepatocyte-like cells such as obtaining morphologic, functional, and αFP and HNF1-α expression patterns which in turn were higher in cells exposed to RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Wharton's Jelly-Mesenchymal Stem Cells (WJ-MSCs) are pluripotent cells with differentiation capability into most cell lineages. The aim of the current work was to examine the role of Retinoic Acid (RA) in differentiation process of these cells into hepatocyte-like cells and determine the morphological and functional patterns.

Methods: Human WJ-MSCs were extracted, cultured and expanded; after approximately 95% of confluence, the cells were treated with hepatogenic media containing RA. The cells were subsequently analyzed for morphological changes, glycogen storage, albumin production, and specific gene expression.

Results: WJ-MSCs expressed high levels of CD90 (93.6%) and CD105 (90.7%), but low levels of CD34 (0.3%) and CD45 (0.8%). Albumin production had significant difference in the two groups (p≤0.05). The data showed specific characteristics in favor of considering the differentiated cells as hepatocyte-like cells such as obtaining morphologic, functional, and αFP and HNF1-α expression patterns which in turn were higher in cells exposed to RA.

Conclusion: Based on the data of present study, RA is an effective molecule in inducing differentiation of WJ-MSCs into hepatocyte-like cells; therefore, it may be considered as a promising factor for targeting therapy of liver disorders.

No MeSH data available.


Related in: MedlinePlus

Morphologic features of Wharton's Jelly-Mesenchymal Stem Cells (WJ-MSCs). A) MSCs isolated from WJ with heterogeneous flattened fibroblast-like characteristics, B) cell expansion with homogenous form, C) and further expansion, D) morphology of colony forming cells (Invert microscope, Scale bar=100 μm).
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Figure 1: Morphologic features of Wharton's Jelly-Mesenchymal Stem Cells (WJ-MSCs). A) MSCs isolated from WJ with heterogeneous flattened fibroblast-like characteristics, B) cell expansion with homogenous form, C) and further expansion, D) morphology of colony forming cells (Invert microscope, Scale bar=100 μm).

Mentions: MSC detachment from WJ was carried out via explant method. The number of cells isolated from each 1 cm length of cord was 10–12×103. At first, the primary segregated cells had heterogeneous flattened fibroblast-like characteristics in terms of shape and processes (Figure 1A). After a few weeks of expansion in medium (Figures 1B and 1C), they obtained homogeneous features with the following colony formation (Figure 1D).


Retinoic Acid as the Stimulating Factor for Differentiation of Wharton's Jelly-Mesenchymal Stem Cells into Hepatocyte-like Cells.

Mortezaee K, Minaii B, Sabbaghziarani F, Ragerdi Kashani I, Hassanzadeh G, Pasbakhsh P, Barbarestani M, Latifpour M - Avicenna J Med Biotechnol (2015 Jul-Sep)

Morphologic features of Wharton's Jelly-Mesenchymal Stem Cells (WJ-MSCs). A) MSCs isolated from WJ with heterogeneous flattened fibroblast-like characteristics, B) cell expansion with homogenous form, C) and further expansion, D) morphology of colony forming cells (Invert microscope, Scale bar=100 μm).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4508333&req=5

Figure 1: Morphologic features of Wharton's Jelly-Mesenchymal Stem Cells (WJ-MSCs). A) MSCs isolated from WJ with heterogeneous flattened fibroblast-like characteristics, B) cell expansion with homogenous form, C) and further expansion, D) morphology of colony forming cells (Invert microscope, Scale bar=100 μm).
Mentions: MSC detachment from WJ was carried out via explant method. The number of cells isolated from each 1 cm length of cord was 10–12×103. At first, the primary segregated cells had heterogeneous flattened fibroblast-like characteristics in terms of shape and processes (Figure 1A). After a few weeks of expansion in medium (Figures 1B and 1C), they obtained homogeneous features with the following colony formation (Figure 1D).

Bottom Line: WJ-MSCs expressed high levels of CD90 (93.6%) and CD105 (90.7%), but low levels of CD34 (0.3%) and CD45 (0.8%).Albumin production had significant difference in the two groups (p≤0.05).The data showed specific characteristics in favor of considering the differentiated cells as hepatocyte-like cells such as obtaining morphologic, functional, and αFP and HNF1-α expression patterns which in turn were higher in cells exposed to RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Wharton's Jelly-Mesenchymal Stem Cells (WJ-MSCs) are pluripotent cells with differentiation capability into most cell lineages. The aim of the current work was to examine the role of Retinoic Acid (RA) in differentiation process of these cells into hepatocyte-like cells and determine the morphological and functional patterns.

Methods: Human WJ-MSCs were extracted, cultured and expanded; after approximately 95% of confluence, the cells were treated with hepatogenic media containing RA. The cells were subsequently analyzed for morphological changes, glycogen storage, albumin production, and specific gene expression.

Results: WJ-MSCs expressed high levels of CD90 (93.6%) and CD105 (90.7%), but low levels of CD34 (0.3%) and CD45 (0.8%). Albumin production had significant difference in the two groups (p≤0.05). The data showed specific characteristics in favor of considering the differentiated cells as hepatocyte-like cells such as obtaining morphologic, functional, and αFP and HNF1-α expression patterns which in turn were higher in cells exposed to RA.

Conclusion: Based on the data of present study, RA is an effective molecule in inducing differentiation of WJ-MSCs into hepatocyte-like cells; therefore, it may be considered as a promising factor for targeting therapy of liver disorders.

No MeSH data available.


Related in: MedlinePlus