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Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells.

Kang MY, Oh TJ, Cho YM - Endocrinol Metab (Seoul) (2015)

Bottom Line: The mitochondria/cytosol ratio was increased from 7.60±3.12% to 10.53±2.70% by exendin-4.Proliferator-activated receptor-gamma coactivator 1α expression was increased approximately 2-fold by GLP-1 treatment.In conclusion, the present study presents evidence for a new mechanism of action by which GLP-1 improves pancreatic β-cell function via enhanced mitochondrial mass and performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT
Glucagon-like peptide-1 (GLP-1) is a gut-derived incretin hormone that increases glucose-stimulated insulin secretion in pancreatic β-cells. Since mitochondrial function is crucial to insulin secretion, we hypothesized that GLP-1 may increase mitochondrial biogenesis in pancreatic β-cells. We treated INS-1 rat insulinoma cells with GLP-1 or exendin-4 for 48 hours and measured mitochondrial mass and function. Both GLP-1 and exendin-4 increased mitochondrial mass by approximately 20%. The mitochondria/cytosol ratio was increased from 7.60±3.12% to 10.53±2.70% by exendin-4. In addition, GLP-1 increased the mitochondrial membrane potential and oxygen consumption. Proliferator-activated receptor-gamma coactivator 1α expression was increased approximately 2-fold by GLP-1 treatment. In conclusion, the present study presents evidence for a new mechanism of action by which GLP-1 improves pancreatic β-cell function via enhanced mitochondrial mass and performance.

No MeSH data available.


Related in: MedlinePlus

Glucagon-like peptide-1 (GLP-1) increases mitochondria membrane potential (A, n=6) and cellular oxygen consumption rate (B, n=4). TMRE, tetramethylrhodamine ethyl ester perchlorate; MFI, mean fluorescence intensity. aP<0.05; bP<0.01 compared with control.
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Figure 2: Glucagon-like peptide-1 (GLP-1) increases mitochondria membrane potential (A, n=6) and cellular oxygen consumption rate (B, n=4). TMRE, tetramethylrhodamine ethyl ester perchlorate; MFI, mean fluorescence intensity. aP<0.05; bP<0.01 compared with control.

Mentions: The intensity of TMRE staining, which is an indicator of the strength of the mitochondrial membrane potential, was increased in INS-1 cells treated with GLP-1 or exendin-4 for 48 hours (Fig. 2A). In line with this finding, the oxygen consumption rate of cells treated with GLP-1 for 48 hours exhibited a significant increase relative to controls (56.3±2.8 µmol/sec/105 cells vs. 42.0±1.6 µmol/sec/105 cells, P<0.05) (Fig. 2B).


Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells.

Kang MY, Oh TJ, Cho YM - Endocrinol Metab (Seoul) (2015)

Glucagon-like peptide-1 (GLP-1) increases mitochondria membrane potential (A, n=6) and cellular oxygen consumption rate (B, n=4). TMRE, tetramethylrhodamine ethyl ester perchlorate; MFI, mean fluorescence intensity. aP<0.05; bP<0.01 compared with control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508267&req=5

Figure 2: Glucagon-like peptide-1 (GLP-1) increases mitochondria membrane potential (A, n=6) and cellular oxygen consumption rate (B, n=4). TMRE, tetramethylrhodamine ethyl ester perchlorate; MFI, mean fluorescence intensity. aP<0.05; bP<0.01 compared with control.
Mentions: The intensity of TMRE staining, which is an indicator of the strength of the mitochondrial membrane potential, was increased in INS-1 cells treated with GLP-1 or exendin-4 for 48 hours (Fig. 2A). In line with this finding, the oxygen consumption rate of cells treated with GLP-1 for 48 hours exhibited a significant increase relative to controls (56.3±2.8 µmol/sec/105 cells vs. 42.0±1.6 µmol/sec/105 cells, P<0.05) (Fig. 2B).

Bottom Line: The mitochondria/cytosol ratio was increased from 7.60±3.12% to 10.53±2.70% by exendin-4.Proliferator-activated receptor-gamma coactivator 1α expression was increased approximately 2-fold by GLP-1 treatment.In conclusion, the present study presents evidence for a new mechanism of action by which GLP-1 improves pancreatic β-cell function via enhanced mitochondrial mass and performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT
Glucagon-like peptide-1 (GLP-1) is a gut-derived incretin hormone that increases glucose-stimulated insulin secretion in pancreatic β-cells. Since mitochondrial function is crucial to insulin secretion, we hypothesized that GLP-1 may increase mitochondrial biogenesis in pancreatic β-cells. We treated INS-1 rat insulinoma cells with GLP-1 or exendin-4 for 48 hours and measured mitochondrial mass and function. Both GLP-1 and exendin-4 increased mitochondrial mass by approximately 20%. The mitochondria/cytosol ratio was increased from 7.60±3.12% to 10.53±2.70% by exendin-4. In addition, GLP-1 increased the mitochondrial membrane potential and oxygen consumption. Proliferator-activated receptor-gamma coactivator 1α expression was increased approximately 2-fold by GLP-1 treatment. In conclusion, the present study presents evidence for a new mechanism of action by which GLP-1 improves pancreatic β-cell function via enhanced mitochondrial mass and performance.

No MeSH data available.


Related in: MedlinePlus