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Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.

Bi J, Tong L, Zhu X, Yang D, Bai C, Song Y, She J - J. Cell. Mol. Med. (2014)

Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

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Related in: MedlinePlus

Images of histological specimens from the lungs of animals under transmission electron microscope (×30,000). (A) control group; (B) KGF-2 group; (C) HVZP group: The alveolar–capillary barrier was severely damaged, including alveolar type I cell swelling (thick black arrowhead), basement membrane exposure (thick white arrowhead) and alveolar–capillary barrier rupture (thin black arrowhead). (D) HVZP+KGF-2 group: the alveolar–capillary barrier disruption was relatively mild.
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fig06: Images of histological specimens from the lungs of animals under transmission electron microscope (×30,000). (A) control group; (B) KGF-2 group; (C) HVZP group: The alveolar–capillary barrier was severely damaged, including alveolar type I cell swelling (thick black arrowhead), basement membrane exposure (thick white arrowhead) and alveolar–capillary barrier rupture (thin black arrowhead). (D) HVZP+KGF-2 group: the alveolar–capillary barrier disruption was relatively mild.

Mentions: Transmission electron microscopy results revealed that compared with control and KGF-2 groups (Fig.6A and B), the alveolar–capillary barrier was severely damaged in HVZP group, including alveolar type I cell swelling, basement membrane exposure and alveolar–capillary barrier rupture (Fig.6C). In the HVZP+KGF-2 group, the alveolar–capillary barrier disruption was relatively mild (Fig.6D).


Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.

Bi J, Tong L, Zhu X, Yang D, Bai C, Song Y, She J - J. Cell. Mol. Med. (2014)

Images of histological specimens from the lungs of animals under transmission electron microscope (×30,000). (A) control group; (B) KGF-2 group; (C) HVZP group: The alveolar–capillary barrier was severely damaged, including alveolar type I cell swelling (thick black arrowhead), basement membrane exposure (thick white arrowhead) and alveolar–capillary barrier rupture (thin black arrowhead). (D) HVZP+KGF-2 group: the alveolar–capillary barrier disruption was relatively mild.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508161&req=5

fig06: Images of histological specimens from the lungs of animals under transmission electron microscope (×30,000). (A) control group; (B) KGF-2 group; (C) HVZP group: The alveolar–capillary barrier was severely damaged, including alveolar type I cell swelling (thick black arrowhead), basement membrane exposure (thick white arrowhead) and alveolar–capillary barrier rupture (thin black arrowhead). (D) HVZP+KGF-2 group: the alveolar–capillary barrier disruption was relatively mild.
Mentions: Transmission electron microscopy results revealed that compared with control and KGF-2 groups (Fig.6A and B), the alveolar–capillary barrier was severely damaged in HVZP group, including alveolar type I cell swelling, basement membrane exposure and alveolar–capillary barrier rupture (Fig.6C). In the HVZP+KGF-2 group, the alveolar–capillary barrier disruption was relatively mild (Fig.6D).

Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus