Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.
Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.Show MeSH
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Mentions: Transmission electron microscopy results revealed that compared with control and KGF-2 groups (Fig.6A and B), the alveolar–capillary barrier was severely damaged in HVZP group, including alveolar type I cell swelling, basement membrane exposure and alveolar–capillary barrier rupture (Fig.6C). In the HVZP+KGF-2 group, the alveolar–capillary barrier disruption was relatively mild (Fig.6D).
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.