Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.
Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.Show MeSH
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Mentions: Under optical microscopy, widespread alveolar wall thickening, massive neutrophil infiltration, haemorrhage and hyline membrane forming were observed in HVZP group (Fig.5A–D). The lung injury score in HVZP group was significantly higher than those in other groups (P < 0.05, Fig.5E). However, in HVZP+KGF-2 group, the lung injury was mild and the score was significantly lower than that in HVZP group (P < 0.05, Fig.5E).
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.