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Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.

Bi J, Tong L, Zhu X, Yang D, Bai C, Song Y, She J - J. Cell. Mol. Med. (2014)

Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

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Images of histological specimens under optical microscope stained with haematoxylin and eosin (×100). (A) Control group; (B) KGF-2 group; (C) HVZP group: Widespread alveolar wall thickening, massive neutrophil infiltration, haemorrhage and hyaline membrane formation were observed; (D) HVZP+KGF-2 group: The lung injury was relatively mild. (E) Lung injury score. Score in HVZP group was significantly higher than those in the control group (P < 0.05). Compared with HVZP, the score in HVZP+KGF-2 group was significantly decreased (P < 0.05). *P < 0.05 versus control group; †P < 0.05 versusHVZP group.
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fig05: Images of histological specimens under optical microscope stained with haematoxylin and eosin (×100). (A) Control group; (B) KGF-2 group; (C) HVZP group: Widespread alveolar wall thickening, massive neutrophil infiltration, haemorrhage and hyaline membrane formation were observed; (D) HVZP+KGF-2 group: The lung injury was relatively mild. (E) Lung injury score. Score in HVZP group was significantly higher than those in the control group (P < 0.05). Compared with HVZP, the score in HVZP+KGF-2 group was significantly decreased (P < 0.05). *P < 0.05 versus control group; †P < 0.05 versusHVZP group.

Mentions: Under optical microscopy, widespread alveolar wall thickening, massive neutrophil infiltration, haemorrhage and hyline membrane forming were observed in HVZP group (Fig.5A–D). The lung injury score in HVZP group was significantly higher than those in other groups (P < 0.05, Fig.5E). However, in HVZP+KGF-2 group, the lung injury was mild and the score was significantly lower than that in HVZP group (P < 0.05, Fig.5E).


Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.

Bi J, Tong L, Zhu X, Yang D, Bai C, Song Y, She J - J. Cell. Mol. Med. (2014)

Images of histological specimens under optical microscope stained with haematoxylin and eosin (×100). (A) Control group; (B) KGF-2 group; (C) HVZP group: Widespread alveolar wall thickening, massive neutrophil infiltration, haemorrhage and hyaline membrane formation were observed; (D) HVZP+KGF-2 group: The lung injury was relatively mild. (E) Lung injury score. Score in HVZP group was significantly higher than those in the control group (P < 0.05). Compared with HVZP, the score in HVZP+KGF-2 group was significantly decreased (P < 0.05). *P < 0.05 versus control group; †P < 0.05 versusHVZP group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508161&req=5

fig05: Images of histological specimens under optical microscope stained with haematoxylin and eosin (×100). (A) Control group; (B) KGF-2 group; (C) HVZP group: Widespread alveolar wall thickening, massive neutrophil infiltration, haemorrhage and hyaline membrane formation were observed; (D) HVZP+KGF-2 group: The lung injury was relatively mild. (E) Lung injury score. Score in HVZP group was significantly higher than those in the control group (P < 0.05). Compared with HVZP, the score in HVZP+KGF-2 group was significantly decreased (P < 0.05). *P < 0.05 versus control group; †P < 0.05 versusHVZP group.
Mentions: Under optical microscopy, widespread alveolar wall thickening, massive neutrophil infiltration, haemorrhage and hyline membrane forming were observed in HVZP group (Fig.5A–D). The lung injury score in HVZP group was significantly higher than those in other groups (P < 0.05, Fig.5E). However, in HVZP+KGF-2 group, the lung injury was mild and the score was significantly lower than that in HVZP group (P < 0.05, Fig.5E).

Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus