Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.
Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.Show MeSH
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Mentions: Surfactant protein-C immunoreactivities were mainly detected in alveolar type II cells, and the immunoreactivity markedly decreased in rats treated with HVZP when compared with the control groups (P < 0.05, Fig.3). Keratinocyte growth factor-2 pre-treatment significantly increased the number of alveolar type II cells (P < 0.05, Fig.3). On the other hand, mRNA expression of SP-A, SP-B and SP-C were significantly lower in HVZP group than control group (P < 0.05, Fig.4A–C), but KGF-2 pre-treatment significantly increased the level of SP-A, SP-B and SP-C mRNA expression group (P < 0.05, Fig.4A–C).
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.